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3.6.5.5: dynamin GTPase

This is an abbreviated version!
For detailed information about dynamin GTPase, go to the full flat file.

Word Map on EC 3.6.5.5

Reaction

GTP
+
H2O
=
GDP
+
phosphate

Synonyms

AtDRP1A, B-dynamin , caSey1p, D100 , dDyn, DLC1, DLP-1, DLP1, DlpA, DlpB, DlpC, Dnm1, DNM1L, DNM2, DNM3, DRP, Drp1, Drp1-long, Drp1-short, DRP1E, DRP5A, Drp6, DrpB, Dyn, Dyn I, Dyn-1, Dyn1, DYN2, Dyn2aa, Dyn2ab, Dyn2ba, Dyn2bb, Dyn3, dynamin, dynamin 1, dynamin 1 GTPase, dynamin 1aa, dynamin 2, dynamin 2aa, dynamin 3, dynamin 3aa, Dynamin BREDNM19 , dynamin family GTPase, dynamin GTPase, dynamin I, dynamin I GTPase, dynamin I guanosine triphosphatase, Dynamin UDNM , Dynamin, brain , Dynamin, testicular , dynamin-1, dynamin-1 GTPase, dynamin-1-like GTPase, dynamin-1-like protein, dynamin-2, dynamin-3, dynamin-like GTPase, dynamin-like guanosine triphosphatase, dynamin-like guanosine-triphosphate hydrolase, dynamin-like myxovirus resistance protein A, dynamin-like protein 1, dynamin-like protein 6, dynamin-related GTPase, dynamin-related protein, dynamin-related protein 1, dynamin-related protein 1 isoform 3, dynamin-related protein 1A, dynamin-related protein 1E, dynamin-related protein 2A, dynamin-related protein B, dynamin-retated GTPase Drp1, dynamin1, dynamin2, dynamin2 GTPase, dynamine-related GTPase, dynein light chain 1, dynI, dynI GTPase, EC 3.6.1.50, fission dynamin, GTP phosphohydrolase, GTPase, GTPase dynamin, GTPase dynamin-1, GTPase dynamin-related protein 1, guanine triphosphatase, guanosine 5'-triphosphatase, guanosine triphosphatase, hDrp1 isoform 3, large GTPase, Mfn1, mitochondrial division dynamin, mitochondrial dynamin, mitochondrial inner membrane fusion dynamin Mgm1, mitofusin 1, mOPA1, multidomain GTPase, Mx GTPase, MX1, MxA, myxovirus resistance GTPase, neurolastin, Nicotiana tabacum dynamin-related protein 3, NtDRP3, OPA1, optic atrophy 1, PfDYN2, phosphatase, guanosine tri-, Protein shibire, ribosomal GTPase, Sey1p, Shibire protein , T-dynamin , Vps1p

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.5 Acting on GTP to facilitate cellular and subcellular movement
                3.6.5.5 dynamin GTPase

Crystallization

Crystallization on EC 3.6.5.5 - dynamin GTPase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant GST-tagged chimeric fusion protein of dynamin-related protein 1A, AtDRP1A, comprising GTPase domain of Arabidopsis thaliana AtDRP1A fused to its GTPase effector domain (GED) in a nucleotide-associated form, hanging drop vapour diffusion method, mixing of 5 mg/ml dimeric protein in 20 mM Tris-HCl, pH 8.0, 150 mM NaC, with 160 mM sodium formate, 16% w/v PEG 3350, 40 mM magnesium chloride, 6% w/v 2-propanol, 20 mM HEPES, pH 7.5, method optimization, X-ray diffraction structure determination and analysis at 3.6 A resolution, molecular replacement using the crystal structure of human dynamin GG dimer, PDB ID 2X2E
purified recombinant truncated enzyme Sey1p(1-692) , wild-type or selenomethionine-labeled, in complex with GDP, GMP-PNP, or GDP plus AlF4-, X-ray diffraction structure determination and analysis at 2.3-3.0 A resolution
GTPase domain and the bundle signalling element of dynamin in the GDP-bound state, GG1, hanging drop vapour diffusion method, mixing of 0.0015 ml of 10 mg/ml GG1 protein solution containing 2 mM GDP and 2 mM MgCl2 with 0.0015 ml of reservoir solution containing 0.1 M Tris, pH 8.0, 26% PEG 3350, and 0.2 M NaSCN, and equilibration against 0.7 ml of reservoir solution, one week, 4°C, X-ray diffraction structure determination and analysis at 1.7-1.8 A resolution, molecular replacement using GG1GDP.AlFx (PDB ID 2X2E) as search model
purified recombinant dynamin-related protein 1 GTPase-GTPase effector domain fusion protein, sitting drop vapour diffusion method, mixing of 0.0004 ml of protein solution and reservoir solution, the latter containing 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 5.5, 25% v/v PEG 3350 or 0.1 M sodium citrate, pH 5.5, 20% PEG 3000, 20°C, X-ray diffraction structure determination and analysis at 2.67 A resolution
purified wild-type and mutants of DNM1L isoform 2, mixing of 0.001 ml of 1.2 mg/ml protein with 0.001 ml of reservoir solution containing 0.1 M sodium citrate pH 5, 27.5% PEG 3000, equilibration against 0.4 ml of reservoir solution, 3-5 days, for co-crystallization of the enzyme with a non-hydrolyzable GTP analogue the protein is incubated with 1 mM GMP-PNP and 4 mM MgCl2, and then purified by gel filtration, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement using human dynamin-1 structure, PDB ID 3SNH
hanging-drop method, 1.9 A crystal structure of the nucleotide-free GTPase domain of dynamin 1
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