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3.6.5.5: dynamin GTPase

This is an abbreviated version!
For detailed information about dynamin GTPase, go to the full flat file.

Word Map on EC 3.6.5.5

Reaction

GTP
+
H2O
=
GDP
 +
phosphate

Synonyms

AtDRP1A, B-dynamin , caSey1p, D100 , dDyn, DLC1, DLP-1, DLP1, DlpA, DlpB, DlpC, Dnm1, DNM1L, DNM2, DNM3, DRP, Drp1, Drp1-long, Drp1-short, DRP1E, DRP5A, Drp6, DrpB, Dyn, Dyn I, Dyn-1, Dyn1, DYN2, Dyn2aa, Dyn2ab, Dyn2ba, Dyn2bb, Dyn3, dynamin, dynamin 1, dynamin 1 GTPase, dynamin 1aa, dynamin 2, dynamin 2aa, dynamin 3, dynamin 3aa, Dynamin BREDNM19 , dynamin family GTPase, dynamin GTPase, dynamin I, dynamin I GTPase, dynamin I guanosine triphosphatase, Dynamin UDNM , Dynamin, brain , Dynamin, testicular , dynamin-1, dynamin-1 GTPase, dynamin-1-like GTPase, dynamin-1-like protein, dynamin-2, dynamin-3, dynamin-like GTPase, dynamin-like guanosine triphosphatase, dynamin-like guanosine-triphosphate hydrolase, dynamin-like myxovirus resistance protein A, dynamin-like protein 1, dynamin-like protein 6, dynamin-related GTPase, dynamin-related protein, dynamin-related protein 1, dynamin-related protein 1 isoform 3, dynamin-related protein 1A, dynamin-related protein 1E, dynamin-related protein 2A, dynamin-related protein B, dynamin-retated GTPase Drp1, dynamin1, dynamin2, dynamin2 GTPase, dynamine-related GTPase, dynein light chain 1, dynI, dynI GTPase, EC 3.6.1.50, fission dynamin, GTP phosphohydrolase, GTPase, GTPase dynamin, GTPase dynamin-1, GTPase dynamin-related protein 1, guanine triphosphatase, guanosine 5'-triphosphatase, guanosine triphosphatase, hDrp1 isoform 3, large GTPase, Mfn1, mitochondrial division dynamin, mitochondrial dynamin, mitochondrial inner membrane fusion dynamin Mgm1, mitofusin 1, mOPA1, multidomain GTPase, Mx GTPase, MX1, MxA, myxovirus resistance GTPase, neurolastin, Nicotiana tabacum dynamin-related protein 3, NtDRP3, OPA1, optic atrophy 1, PfDYN2, phosphatase, guanosine tri-, Protein shibire, ribosomal GTPase, Sey1p, Shibire protein , T-dynamin , Vps1p

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.5 Acting on GTP to facilitate cellular and subcellular movement
                3.6.5.5 dynamin GTPase

Purification

Purification on EC 3.6.5.5 - dynamin GTPase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DLP1 and its variants are expressed as glutathione-S-transferase-fusion proteins in Escherichia coli and are purified with glutathione-Sepharose beads
-
glutathione S-transferase dynamin-1 fusion constructs are expressed in Escherichia coli BL21 cells, the proteins are purified using glutathione-Sepharose 4B beads
-
glutathione S-transferase-fused proteins are purified from Escherichia coli strain BL21 using glutathione-Sepharose, full-length dynamin is purified from rat brain
-
native enzyme from sheep brain membrane fraction by affinity chromatography
-
recombinant C-terminally His6-tagged isozyme Dyn1 from Sf9 cells by nickel affinity chromatography and dialysis
recombinant C-terminally His6-tagged isozyme Dyn2 from Sf9 cells by nickel affinity chromatography and dialysis
recombinant C-terminally His6-tagged isozyme Dyn3 from Sf9 cells by nickel affinity chromatography and dialysis
recombinant dynamin 1 GTPase domain GG1 from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography, tag cleavage by TEV protease, ultrafiltration, and gel filtration
recombinant dynamin-1
-
recombinant GST- and His-tagged MxA from Escherichia coli strain BL21(DE3) by nickel affinity and glutathione affinity chromatography, and gel filtration
recombinant GST-tagged chimeric fusion protein of AtDRP1A fusion protein from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
recombinant GST-tagged wild-type and mutant GST-SEY1 from Escherichia coli strain BL21 by glutathione affinity chromatography, the tag is cleaved off by thrombin
-
recombinant His-Drp1 proteins are purified by metal-chelating resin and further purified by ion exchange chromatography
-
recombinant His-tagged DLP-1 isozyme 2 from Escherichia coli strain C41 (DE3) by nickel affinity chromatography, anion exchange chromatography, and gel filtration
-
recombinant N-terminally Strep-tagged Drp1 from Escherichia coli strain BL21 Star (DE3) by affinity chromatography, followed by tag cleavage by HRV3C protease, and gel filtration
recombinant N-terminally Strep-tagged Drp1 from Saccharomyces cerevisiae strain JSY9612 by affinity chromatography, followed by tag cleavage by PreScission protease, and dialysis
recombinant nucleotide-free GTPase-GTPase effector domain fusion protein of human Drp1, and wild-type and mutant full-length enzymes from Escherichia coli strain BL21(DE3)
recombinant truncated enzyme Sey1p(1-692) from Escherichia coli
recombinant wild-type and K44A mutant dynamin-1, expressed in Sf9 cells
-
TALON metal affinity resin column chromatography and MonoQ column chroamtography
-
using a nickel-agarose column
using amylose affinity chromatography, cleavage of the MBP fusion tag
-
using Ni-NTA and hydroxyapatite chromatography
-