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3.4.24.56: insulysin

This is an abbreviated version!
For detailed information about insulysin, go to the full flat file.

Word Map on EC 3.4.24.56

Reaction

Degradation of insulin, glucagon and other polypeptides. No action on proteins =

Synonyms

ADE, amyloid degrading enzyme, cgd6_5510, EC 3.4.22.11, EC 3.4.99.10, EC 3.4.99.45, gamma-endorphin-generating enzyme, IDE, INS20-19, insulin degrading enzyme, Insulin protease, Insulin proteinase, Insulin-degrading enzyme, Insulin-degrading neutral proteinase, Insulin-glucagon protease, Insulin-specific protease, Insulinase, Insulysin, Metalloinsulinase, More, pitrilysin metallopeptidase 1, Pitrm1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.56 insulysin

Cloned

Cloned on EC 3.4.24.56 - insulysin

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a form of the enzyme derived from an alternative translational start site that can localize to mitochondria instead of the cytosol
cDNA cloned and expressed
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cDNA cloned and overexpressed in human embryonic kidney 293 cells
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cloning from C6 glioma cells, expression of recombinant GST-tagged wild-type IDE, and of recombinant IDE mutant E111Q
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determination of allele frequencies for Caucasians and Japanese, genotyping, single nucleotide polymorphism in the IDE gene, no association of IDE haplotypes with the risk of dementia, overview
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DNA and amino acid sequence determination and analysis, genotyping, overview
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Drosophila melanogaster
Eimeria bovis
expressed as hexahistidine fusion proteins in SF9 insect cell
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expressed in and isolated from Escherichia coli as C-terminal polyhistidine tagged fusion partners lacking N-terminal mitochondrial targeting sequence
expressed in bacteria
-
expressed in BV-2 cells
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expressed in Escherichia coli
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expressed in Escherichia coli Rosetta (DE3) cells
expression in cells of Spodoptera frugiperda
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expression in CHO cell
expression in Escherichia coli
expression in Escherichia coli BL21
expression of cgd6_5510 in Escherichia coli
expression of His-tagged IDE in Escherichia coli strain Rosetta (DE3)
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expression of His-tagged wild-type and mutant IDEs in Escherichia coli strain Rosetta (DE3)
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expression of the enzyme in transgenic mice under control of the H1 CMV promoter
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expression of the N-terminally His6-tagged enzyme in Spodopterafrugiperda Sf9 cells using the baculovirus transfection system
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expression of wild-type and mutant enzymes in Escherichia coli
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expression of wild-type and mutant enzymes in HEK-293swe.3 cells, that stably expresses Myc-epitope tagged human APP-695 harboring the FAD-linked, socalled Swedish mutation
genetic linkage and association of Alzheimer disease on chromosome 10q23-24 in the region harboring the IDE gene
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genotyping, IDE shows a large genetic variability
glutathione S-transferase-tagged human insulin-degrading enzyme in pGEX-6p-1 vector is transformed into Escherichia coli BL21-CodonPlus (DE3) competent cells
human
IDE expression analysis in neuron tissue, overview
IDE gene is localized on chromosome 10q24, genotyping of IDE in the Finnish population, overview
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IDE gene, genotyping in Chinese population, Shanghai, China
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IDE genotyping, expression of promoter constructs in Hela cells and SH-SY5Y cells
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IDE is expressed in Sf9 insect cells by using baculovirus system
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polyhistidine- and haemagglutinin-tagged recombinant wild-type and catalytically inactive mutants expressed in bacteria
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recombinant enzyme produced in Sf9 cells
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recombinant expression of His6-tagged IDE in Escherichia coli strain BL21(DE3)
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recombinantly expressed
the gene encoding IDE is located on chromosome 10q23-q25, a gene locus linked to schizophrenia
-
wild-type and E111Q glutathione S-transferase tagged human insulin-degrading enzyme are expressed in Escherichia coli BL21 (DE3) codon plus competent cells