3.4.24.56: insulysin
This is an abbreviated version!
For detailed information about insulysin, go to the full flat file.
Word Map on EC 3.4.24.56
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3.4.24.56
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alzheimer
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neprilysin
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abeta
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hippocampus
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dementia
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mellitus
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cerebral
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morris
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amyloid-beta
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metalloprotease
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maze
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neuroprotective
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amyloidogenic
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tau
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beta-amyloid
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neurodegenerative
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presenilin
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endothelin-converting
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senile
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gamma-secretase
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beta-protein
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125i-insulin
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abeta-degrading
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hyperinsulinemia
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metalloendopeptidase
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bacitracin
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beta-secretase
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late-onset
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amylin
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microglia
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medicine
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adam10
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non-amyloidogenic
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glutathione-insulin
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enzyme-1
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b-chains
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analysis
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ad-like
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anti-ide
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exosite
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beta-peptide
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abeta1-40
- 3.4.24.56
- alzheimer
- neprilysin
- abeta
- hippocampus
- dementia
- mellitus
- cerebral
-
morris
- amyloid-beta
- metalloprotease
-
maze
-
neuroprotective
-
amyloidogenic
- tau
- beta-amyloid
- neurodegenerative
-
presenilin
-
endothelin-converting
-
senile
- gamma-secretase
- beta-protein
- 125i-insulin
-
abeta-degrading
- hyperinsulinemia
- metalloendopeptidase
- bacitracin
- beta-secretase
-
late-onset
- amylin
- microglia
- medicine
- adam10
-
non-amyloidogenic
-
glutathione-insulin
- enzyme-1
- b-chains
- analysis
-
ad-like
-
anti-ide
-
exosite
- beta-peptide
- abeta1-40
Reaction
Degradation of insulin, glucagon and other polypeptides. No action on proteins =
Synonyms
ADE, amyloid degrading enzyme, cgd6_5510, EC 3.4.22.11, EC 3.4.99.10, EC 3.4.99.45, gamma-endorphin-generating enzyme, IDE, INS20-19, insulin degrading enzyme, Insulin protease, Insulin proteinase, Insulin-degrading enzyme, Insulin-degrading neutral proteinase, Insulin-glucagon protease, Insulin-specific protease, Insulinase, Insulysin, Metalloinsulinase, More, pitrilysin metallopeptidase 1, Pitrm1
ECTree
3.4.24.56 insulysinAdvanced search results
results (
results (Turnover Number
Turnover Number on EC 3.4.24.56 - insulysin
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.028
(7-methoxycoumarin-4-yl)acetyl-NPPGFSAFK-2,4-dinitrophenyl
-
pH not specified in the publication, 37°C
0.23
(7-methoxycoumarin-4-yl)acetyl-RPPGFSAFK(2,4-dinitrophenyl)-OH
37°C, pH not specified in the publication
0.0022
amyloid beta-peptide 1-40
pH not specified in the publication, temperature not specified in the publication
-
0.0004
amyloid beta-peptide 1-42
pH not specified in the publication, temperature not specified in the publication
-
61 - 62.7
Dabcyl-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Glu(EDANS)-NH2
1.2
(7-methoxycoumarin-4-yl)acetyl-KLVFFAEDK(Dnp)-OH
pH 7.0, 37°C, mutant enzyme R183Q
7.5
(7-methoxycoumarin-4-yl)acetyl-KLVFFAEDK(Dnp)-OH
pH 7.0, 37°C, wild-type enzyme
0.29
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F820Y
0.298
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme Y150K
0.33
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme 141A
0.338
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme Y150F
0.45
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme Y150W
0.53
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F202D
0.57
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme W199Y
0.57
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, wild-type enzyme
0.625
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme 141W
0.72
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F115Y
0.725
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F115A
0.73
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F115W
0.735
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F202Y
0.78
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme 141Y
0.803
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F202W
0.85
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F820W
1.1
(7-methoxycoumarin-4-yl)acetyl-QKLVFFAEDVK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme Y150F
0.088
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme W199K
0.095
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme Y150K
0.097
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F202D
0.097
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F202K
0.1
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F141W
0.108
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F820Y
0.123
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F141N
0.143
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme Y150W
0.148
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F202W
0.16
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F820W
0.16
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme W199Y
0.17
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F202N
0.177
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme W199N
0.178
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F141Y
0.18
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme W199A
0.198
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, wild-type enzyme
0.2
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F115Y
0.22
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F141A
0.22
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme Y150F
0.24
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme F202Y
0.24
(7-methoxycoumarin-4-yl)acetyl-VEALYLVCGEK(2,4-dinitrophenyl)-OH
pH 7.3, 37°C, mutant enzyme W199F
2 - 3.7
2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
mutant H112D, pH 7.3
2.4
2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
mutant E111V, pH 7.3
6.5
2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
mutant E111L, pH 7.3
11.4
2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
mutant E111F, pH 7.3
17.5
2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
mutant E111A, pH 7.3
1953
2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
mutant H112Q, pH 7.3
162600
2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine)
-
wild-type, pH 7.3
1.2
7-methoxycoumarin-4-yl-acetyl-RPPGFSAFK-2,4-dinitrophenyl
pH 7.0, 37°C, mutant enzyme R183Q
6.5
7-methoxycoumarin-4-yl-acetyl-RPPGFSAFK-2,4-dinitrophenyl
pH 7.0, 37°C, wild-type enzyme
0.003
Abz-GGFLRKHGQ-EDDnp
-
pH 7.4, 37°C, mutant Y609F IDE:IDE mutant E111F/Y609F
0.01
Abz-GGFLRKHGQ-EDDnp
-
pH 7.4, temperature not specified in the publication, mutant DELTAC lacking the C-terminal region
0.043
Abz-GGFLRKHGQ-EDDnp
-
pH 7.4, temperature not specified in the publication, wild-type
0.06
Abz-GGFLRKHGQ-EDDnp
-
pH 7.4, 37°C, mutant Y609F IDE:IDE mutant H112Q/Y609F
0.078
Abz-GGFLRKHGQ-EDDnp
-
mutant R429S, pH 7.4, temperature not specified in the publication
0.086
Abz-GGFLRKHGQ-EDDnp
-
wild-type, pH 7.4, temperature not specified in the publication
0.258
Abz-GGFLRKHGQ-EDDnp
-
mutant K898A/K899A/S901A, pH and temperature not specified in the publication
0.72
Abz-GGFLRKHGQEDDnp
-
pH 7.4, 37°C, 56 kDa detagged fragment of recombinant His6-tagged enzyme
0.77
Abz-GGFLRKHGQEDDnp
-
pH 7.4, 37°C, 76 kDa detagged fragment of recombinant His6-tagged enzyme
93.17
Abz-GGFLRKHGQEDDnp
-
pH 7.4, 37°C, recombinant His6-tagged full-length enzyme
104
Abz-GGFLRKHGQEDDnp
-
pH 7.4, 37°C, recombinant His6-tagged full-length enzyme
20
amyloid beta-peptide1-40
recombinant mutant D426C/K899C, pH 7.4, 37°C
-
20
amyloid beta-peptide1-40
-
recombinant mutant D426C/K899C, pH 7.4, 37°C
-
61
Dabcyl-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Glu(EDANS)-NH2
-
pH 7.3, 37°C, presence of 0.04 mM somatostatin
62.7
Dabcyl-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Glu(EDANS)-NH2
-
pH 7.3, 37°C
