Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.56 extracted from

  • McCord, L.; Liang, W.; Dowdell, E.; Kalas, V.; Hoey, R.; Koide, A.; Koide, S.; Tang, W.
    Conformational states and recognition of amyloidogenic peptides of human insulin-degrading enzyme (2013), Proc. Natl. Acad. Sci. USA, 110, 13827-13832.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.56

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M sodium cacodylate (pH 6.5), 0.2 M MgCl2, and 10% (w/v) PEG-3000 at 18°C Homo sapiens

Protein Variants

Protein Variants Comment Organism
F530A the mutation renders the enzyme hyperactive, with up to a 20fold enhancement in degrading activity Homo sapiens
G361A/G362A the mutant has reduced enzymatic activity Homo sapiens
P284G the mutation results in a slight reduction of enzyme catalysis Homo sapiens
P286G the mutation results in a significant reduction of enzyme catalysis Homo sapiens
P289G the mutant shows wild type activity Homo sapiens
P292G the mutation results in intermediate reduction of enzyme catalysis Homo sapiens
R767A the mutant exists mostly as a monomer Homo sapiens
S132C/E817C the mutant preferentially stays in the closed state Homo sapiens
Y496A the mutation dramatically impairs the enzymatic activity Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amyloid beta-peptide + H2O Homo sapiens
-
 ?
-
 ?
insulin + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P14735
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Source Q column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methoxycoumarin-4-yl-acetyl-RPPGF-SAFK-2,4-dinitrophenyl + H2O
-
 Homo sapiens ?
-
 ?
amyloid beta-peptide + H2O
-
 Homo sapiens ?
-
 ?
insulin + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer X-ray scattering Homo sapiens

Synonyms

Synonyms Comment Organism
IDE
-
 Homo sapiens
Insulin-degrading enzyme
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function insulin-degrading enzyme selectively degrades the monomer of amyloidogenic peptides and contributes to clearance of amyloid-beta. Thus, the enzyme retards the progression of Alzheimer’s disease Homo sapiens