Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D426A | mutation diminishes activity | Homo sapiens |
F807A | mutation decreass the Km-value of the amyloid beta substrate | Homo sapiens |
K364A | mutation does not change the activity | Homo sapiens |
K898A | mutation decreass the Km-value of the amyloid beta substrate | Homo sapiens |
K898A | mutation results in increased catalytic activity | Homo sapiens |
S137A | mutation decreass the Km-value of the amyloid beta substrate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, wild-type enzyme | Homo sapiens | |
0.035 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme K364A | Homo sapiens | |
0.035 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme S137A | Homo sapiens | |
0.043 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme K898A | Homo sapiens | |
0.044 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme F807A | Homo sapiens | |
0.103 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme D426A | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amyloid beta + H2O | Homo sapiens | role of insulin-degrading enzyme in the intracytosolic clearance of amyloid beta and other amyloid-like peptides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14735 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-GGFLRKHGQ-EDDnp + H2O | - |
Homo sapiens | ? | - |
? | |
amyloid beta + H2O | - |
Homo sapiens | ? | - |
? | |
amyloid beta + H2O | role of insulin-degrading enzyme in the intracytosolic clearance of amyloid beta and other amyloid-like peptides | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDE | - |
Homo sapiens |
Insulin-degrading enzyme | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.13 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme D426A | Homo sapiens | |
1.68 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme F807A | Homo sapiens | |
1.7 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, wild-type enzyme | Homo sapiens | |
1.83 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme K364A | Homo sapiens | |
1.97 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme S137A | Homo sapiens | |
2.42 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme K898A | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | role of insulin-degrading enzyme in the intracytosolic clearance of amyloid beta and other amyloid-like peptides | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.97 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme D426A | Homo sapiens | |
38.18 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme F807A | Homo sapiens | |
51.5 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, wild-type enzyme | Homo sapiens | |
52.3 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme K364A | Homo sapiens | |
56.3 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme K898A | Homo sapiens | |
56.3 | - |
Abz-GGFLRKHGQ-EDDnp | pH 7.7, 37°C, mutant enzyme S137A | Homo sapiens |