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Literature summary for 3.4.24.56 extracted from

  • Ralat, L.A.; Kalas, V.; Zheng, Z.; Goldman, R.D.; Sosnick, T.R.; Tang, W.J.
    Ubiquitin is a novel substrate for human insulin-degrading enzyme (2011), J. Mol. Biol., 406, 454-466.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.56

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
E341A mutant is active in degrading substrate V, relative activity closed to wild-type Homo sapiens
E341K mutant is active in degrading substrate V, relative activity 15% compared to wild-type. Exosite mutant is unable to further degrade the Ub1-74 fragment Homo sapiens
E341Q mutant is active in degrading substrate V, relative activity 20% compared to wild-type. Exosite mutant is unable to further degrade the Ub1-74 fragment Homo sapiens
G339P mutant is active in degrading substrate V, relative activity 75% compared to wild-type. Exosite mutant is unable to further degrade the Ub1-74 fragment Homo sapiens
G361P mutant is active in degrading substrate V, relative activity 80% compared to wild-type. Exosite mutant is unable to further degrade the Ub1-74 fragment Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
nestin potently inhibits the cleavage of ubiquitin by IDE Homo sapiens
Ub1-72 cleaved ubiquitin Homo sapiens
Ub1-74 cleaved ubiquitin Homo sapiens
ubiquitin
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)acetyl-NPPGFSAFK-2,4-dinitrophenyl + H2O bradykinin mimetic substrate V Homo sapiens ?
-
 ?
ubiquitin + H2O IDE cleaves ubiquitin in a biphasic manner, first, by rapidly removing the two C-terminal glycines (kcat = 2/sec) followed by a slow cleavage between residues 72-73 (kcat = 0.07/sec), thereby producing the inactive Ub1-74 and Ub1-72 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
IDE
-
 Homo sapiens
Insulysin
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.028
-
 (7-methoxycoumarin-4-yl)acetyl-NPPGFSAFK-2,4-dinitrophenyl pH not specified in the publication, 37°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.001
-
 pH not specified in the publication, 37°C Homo sapiens Ub1-72
0.09
-
 pH not specified in the publication, 37°C Homo sapiens ubiquitin
0.1
-
 pH not specified in the publication, 37°C Homo sapiens Ub1-72