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3.4.22.41: cathepsin F

This is an abbreviated version!
For detailed information about cathepsin F, go to the full flat file.

Word Map on EC 3.4.22.41

Reaction

The recombinant enzyme cleaves synthetic substrates with Phe and Leu (better than Val) in P2, with high specificity constant (kcat/Km) comparable to that of cathepsin L =

Synonyms

cat F, CatF, cathepsin f, cathepsin F-1, CATSF, CF-1, CF-11, CF-4, CF-6, cf1, CtF, CTSF, Ov-CF-1, PwCP1, PwCP10, PwCP11, PwCP2, PwCP3, PwCP4, PwCP5, PwCP6, PwCP7, PwCP8, PwCP9, Tci-CF-1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.41 cathepsin F

Cloned

Cloned on EC 3.4.22.41 - cathepsin F

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA cloned from primary HSCs using RACE-PCR
chromosomal localization of the gene endocing cathepsin F at 11q13
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DNA and amino acid sequence determination and analysis
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DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
enhanced production and release of cathepsins in tumor cells leads to tumor cell growth, invasion and metastasis
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expressed in Escherichia coli DH5alphaMCR cells using the pGEX-4 T-1 expression vector system
expressed in Escherichia coli HEK-293T cells
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expressed in Escherichia coli M15 (pREP4) cells
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expressed in Escherichia coli M15 [pREP4] cells
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expression of mature enzyme in Escherichia coli
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PCR with primers specific for the Camellia sinensis cathepsin F gene, 5'- and 3'-RACE used to clone and identify a coding sequence of 981 bp (AY821800). The Ov-cf-1 gene is organized into 7 exons. Phylogenetic analysis confirms relationship of Ov-CF-1 with other cathepsin F proteases.