Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.41 extracted from

  • Pinlaor, P.; Kaewpitoon, N.; Laha, T.; Sripa, B.; Kaewkes, S.; Morales, M.E.; Mann, V.H.; Parriott, S.K.; Suttiprapa, S.; Robinson, M.W.; To, J.; Dalton, J.P.; Loukas, A.; Brindley, P.J.
    Cathepsin F cysteine protease of the human liver fluke, Opisthorchis viverrini (2009), PLoS Negl. Trop. Dis., 3, e398.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.41

Cloned(Commentary)

Cloned (Comment) Organism
PCR with primers specific for the Camellia sinensis cathepsin F gene, 5'- and 3'-RACE used to clone and identify a coding sequence of 981 bp (AY821800). The Ov-cf-1 gene is organized into 7 exons. Phylogenetic analysis confirms relationship of Ov-CF-1 with other cathepsin F proteases. Opisthorchis viverrini

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23810
-
 predicted from amino acid sequence, 213 residues Opisthorchis viverrini

Organism

Organism UniProt Comment Textmining
Opisthorchis viverrini Q5PXS3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Opisthorchis viverrini
additional information recombinant expression results in zymogen which needs to be activated, recombinant Ov-CF-1 does not autocatalytically process and activate at pH 4.5, activation can be accomplished by Fasciola hepatica cathepsin L1 Opisthorchis viverrini

Source Tissue

Source Tissue Comment Organism Textmining
egg RT-PCR, immunohistochemistry Opisthorchis viverrini
-
gut immunohistochemistry Opisthorchis viverrini
-
immature cell RT-PCR Opisthorchis viverrini
-
mature cell RT-PCR Opisthorchis viverrini
-
metacercaria RT-PCR Opisthorchis viverrini
-
testis immunohistochemistry Opisthorchis viverrini
-
vitellarium immunohistochemistry Opisthorchis viverrini
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Leu-Arg-4-methylcoumarin-7-amide + H2O
-
 Opisthorchis viverrini benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide + H2O
-
 Opisthorchis viverrini benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
cathepsin f
-
 Opisthorchis viverrini
Ov-CF-1
-
 Opisthorchis viverrini

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 substrate benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide Opisthorchis viverrini

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 substrate benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide Opisthorchis viverrini
4.5 6 modest enzyme activity, substrates hemoglobin and gelatin Opisthorchis viverrini