3.4.22.41: cathepsin F
This is an abbreviated version!
For detailed information about cathepsin F, go to the full flat file.
Word Map on EC 3.4.22.41
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3.4.22.41
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cathepsins
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diagnostics
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westermani
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viroid
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paragonimus
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f-like
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food industry
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metalloproteinase-10
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cystatin-like
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viverrini
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medicine
- 3.4.22.41
- cathepsins
- diagnostics
- westermani
- viroid
- paragonimus
-
f-like
- food industry
- metalloproteinase-10
-
cystatin-like
- viverrini
- medicine
Reaction
The recombinant enzyme cleaves synthetic substrates with Phe and Leu (better than Val) in P2, with high specificity constant (kcat/Km) comparable to that of cathepsin L =
Synonyms
cat F, CatF, cathepsin f, cathepsin F-1, CATSF, CF-1, CF-11, CF-4, CF-6, cf1, CtF, CTSF, Ov-CF-1, PwCP1, PwCP10, PwCP11, PwCP2, PwCP3, PwCP4, PwCP5, PwCP6, PwCP7, PwCP8, PwCP9, Tci-CF-1
ECTree
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Renatured
Renatured on EC 3.4.22.41 - cathepsin F
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for isozyme CF-4, the purified recombinant protein (10 mg) is slowly added to 1 l of 100 mM Tris-HCl (pH 8.0) containing 1 mM EDTA, 250 mM L-arginine, 5 mM reduced glutathione, and 1 mM oxidized glutathione and gently stirred at 4°C for 20 h. Isozyme CF-11 is refolded in 100 mM Tris-HCl (pH 8.0) containing 1 mM EDTA, 250 mM L-arginine, 10 mM reduced glutathione, and 1 mM oxidized glutathione with the same method
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