Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour diffusion method, mutant cathepsin F, N97Q/N108Q/N170Q, complexed with an irreversible vinyl sulfone inhibitor | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N97Q/N108Q/N170Q | KM-value for benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide is 2fold higher than that of the wild-type enzyme, the turnover number is 2fold lower | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide | pH 6.5, room temperature, wild-type enzyme | Homo sapiens | |
0.043 | - |
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide | pH 6.5, room temperature, mutant enzyme N97Q/N108Q/N170Q | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains three potential N-linked glycosylation sites in the mature region: N97 and N108 and N170 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide + H2O | - |
Homo sapiens | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide | pH 6.5, room temperature, mutant enzyme N97Q/N108Q/N170Q | Homo sapiens | |
2.9 | - |
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide | pH 6.5, room temperature, wild-type enzyme | Homo sapiens |