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Literature summary for 3.4.22.41 extracted from

  • Ho, J.D.; Meltser, Y.; Buggy, J.J.; Palmer, J.T.; Elrod, K.C.; Chan, H.; Mortara, K.D.; Somoza, J.R.
    Expression, purification, crystallization and preliminary X-ray diffraction studies of human cathepsin F complexed with an irreversible vinyl sulfone inhibitor (2002), Acta Crystallogr. Sect. D, 58, 2187-2190.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion method, mutant cathepsin F, N97Q/N108Q/N170Q, complexed with an irreversible vinyl sulfone inhibitor Homo sapiens

Protein Variants

Protein Variants Comment Organism
N97Q/N108Q/N170Q KM-value for benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide is 2fold higher than that of the wild-type enzyme, the turnover number is 2fold lower Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide pH 6.5, room temperature, wild-type enzyme Homo sapiens
0.043
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 6.5, room temperature, mutant enzyme N97Q/N108Q/N170Q Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme contains three potential N-linked glycosylation sites in the mature region: N97 and N108 and N170 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide + H2O
-
Homo sapiens benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide pH 6.5, room temperature, mutant enzyme N97Q/N108Q/N170Q Homo sapiens
2.9
-
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide pH 6.5, room temperature, wild-type enzyme Homo sapiens