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Literature summary for 3.4.22.41 extracted from

  • Maubach, G.; Lim, M.C.; Zhuo, L.
    Nuclear cathepsin F regulates activation markers in rat hepatic stellate cells (2008), Mol. Biol. Cell, 19, 4238-4248.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.41

Cloned(Commentary)

Cloned (Comment) Organism
cDNA cloned from primary HSCs using RACE-PCR Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information downregulation of cathepsin F mRNA by E-64d; treatment with retinol/palmitic acid increases mRNA-level of cystatin B coupled with decrease in cathepsin F activity; treatment with retinol/palmitic acid increases mRNA-level of cystatin C coupled with decrease in cathepsin F activity Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol Western blot Rattus norvegicus 5829
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nucleus immunofluorescence microscopy, fluorescence-labeled peptide substrate (benzyloxycarbonyl-Leucin-Arginin-4-methylcoumaryl-7-amide) with excitation wavelength 380 nm and emission wavelength 460 nm Rattus norvegicus 5634
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nucleus Western blot, immunofluorescence microscopy, fluorescence-labeled peptide substrate (benzyloxycarbonyl-Leu-Arg-4-methylcoumaryl-7-amide) with excitation wavelength 380 nm and emission wavelength 460 nm Rattus norvegicus 5634
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q499S6 Wistar rat
-

Source Tissue

Source Tissue Comment Organism Textmining
hematopoietic stem cell
-
 Rattus norvegicus
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HSC-2 cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 adding E-64d (inhibitor) results in drastically inhibition of enzyme activity in cytosol and nucleus, fluorescence-labeled peptide substrate (benzyloxycarbonyl-Leu-Arg-4-methylcoumaryl-7-amide) with excitation wavelength 380 nm and emission wavelength 460 nm, confirmed by cathepsin F siRNA treatment Rattus norvegicus
additional information
-
 higher activity in nucleus than in cytosol, fluorescence-labeled peptide substrate (benzyloxycarbonyl-Leu-Arg-4-methylcoumaryl-7-amide) with excitation wavelength 380 nm and emission wavelength 460 nm, differentiation between cathepsin F and other cathepsins by using specific inhibitors and substrates Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Rattus norvegicus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Leu-Arg-4-methylcoumaryl-7-amide + H2O
-
 Rattus norvegicus benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
cathepsin f
-
 Rattus norvegicus