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0.0507 - 3.9
Arg-Gly-Arg-4-nitroanilide
0.0867 - 0.0965
benzoyl-Ile-Glu(piperidineamide)-Gly-Arg-p-nitroanilide
0.175 - 0.189
benzoyl-Ile-Glu-(-H/OMe)-Gly-Arg-p-nitroanilide
0.0013
D-cyclohexyl-Gly-L-Pro-L-Arg-4-nitroanilide
-
20 mM Tris-HCl buffer, pH 7.4, containing 100 mM NaCl, 2.5 mM CaCl2 and 0.1% (w/v) PEG 8000 at 25°C
0.0022
D-hexahydrotyrosol-L-Ala-L-Arg-4-nitroanilide
-
20 mM Tris-HCl buffer, pH 7.4, containing 100 mM NaCl, 2.5 mM CaCl2 and 0.1% (w/v) PEG 8000 at 25°C
0.0013
D-phenylalanyl-L-pipecolyl-L-arginine-4-nitroanilide
-
20 mM Tris-HCl buffer, pH 7.4, containing 100 mM NaCl, 2.5 mM CaCl2 and 0.1% (w/v) PEG 8000 at 25°C
0.00016
FPR-prothrombin
-
pH 7.7, 37°C, in presence of phospholipid at 0.025 mM
-
0.00058 - 0.00078
fragment 1.2:prethrombin-2
-
0.036 - 0.24
L-Arg-Gly-L-Arg-4-nitroanilide
0.00065 - 0.00095
meizothrombin
-
0.061
methoxycarbonyl-D-cyclohexylglycyl-Gly-L-Arg-4-nitroanilide
-
20 mM Tris-HCl buffer, pH 7.4, containing 100 mM NaCl, 2.5 mM CaCl2 and 0.1% (w/v) PEG 8000 at 25°C
0.072 - 1.38
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
0.042 - 0.122
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
0.0045
N6-CBZ-D-Lys-L-Pro-L-Arg-4-nitroanilide
-
20 mM Tris-HCl buffer, pH 7.4, containing 100 mM NaCl, 2.5 mM CaCl2 and 0.1% (w/v) PEG 8000 at 25°C
0.0377
Nalpha-benzyloxycarbonyl-L-Lys-p-nitrophenyl ester
-
-
0.0052 - 0.0069
prethrombin-1
-
0.0088
prethrombin-2
-
prothrombinase complex, pH 7.4, 22°C
-
0.000018 - 0.0083
prothrombin
-
0.000071
ProTS195A
-
pH 7.7, 37°C, versus prothrombin, in absence of phospholipid
-
0.00036 - 0.00045
recombinant prothrombin R155A/R284A/R271A mutant rMZ-II
-
0.00039 - 0.0005
recombinant prothrombin R155A/R284A/R320A mutant rP2-II
-
0.026
S-2765
-
wild type enzyme, at pH 7.5 and 25°C
0.097 - 0.206
tosyl-glycyl-prolyl-D-arginine-4-nitroanilide
additional information
additional information
-
0.0507
Arg-Gly-Arg-4-nitroanilide
-
mutant K222A, pH 7.5
0.0517
Arg-Gly-Arg-4-nitroanilide
-
wild-type, pH 7.5
0.191
Arg-Gly-Arg-4-nitroanilide
-
mutant R221A, pH 7.5
0.95
Arg-Gly-Arg-4-nitroanilide
-
mutant E217Q, pH 7.5, presence of 0.5 M NaCl
2
Arg-Gly-Arg-4-nitroanilide
-
mutant E217A, pH 7.5, presence of 0.5 M NaCl
2.05
Arg-Gly-Arg-4-nitroanilide
-
mutant E217Q, pH 7.5
3.9
Arg-Gly-Arg-4-nitroanilide
-
mutant E217A, pH 7.5
0.0867
benzoyl-Ile-Glu(piperidineamide)-Gly-Arg-p-nitroanilide
-
-
0.0867
benzoyl-Ile-Glu(piperidineamide)-Gly-Arg-p-nitroanilide
-
native enzyme
0.0965
benzoyl-Ile-Glu(piperidineamide)-Gly-Arg-p-nitroanilide
-
enzyme from which the residues 1-44 of the light chain have been removed by chymotryptic proteolysis
0.175
benzoyl-Ile-Glu-(-H/OMe)-Gly-Arg-p-nitroanilide
-
native enzyme
0.189
benzoyl-Ile-Glu-(-H/OMe)-Gly-Arg-p-nitroanilide
-
enzyme from which the residues 1-44 of the light chain have been removed by chymotryptic proteolysis
0.00058
fragment 1.2:prethrombin-2
-
pH 7.4, 22°C, in presence of 20 mM factor Va
-
0.00078
fragment 1.2:prethrombin-2
-
pH 7.4, 22°C, in absence of factor Va
-
0.036
L-Arg-Gly-L-Arg-4-nitroanilide
-
25°C
0.06
L-Arg-Gly-L-Arg-4-nitroanilide
-
25°C
0.07
L-Arg-Gly-L-Arg-4-nitroanilide
-
25°C
0.24
L-Arg-Gly-L-Arg-4-nitroanilide
-
25°C
0.00065
meizothrombin
-
pH 7.4, 22°C, in presence of 20 mM factor Va
-
0.00095
meizothrombin
-
pH 7.4, 22°C, in absence of factor Va
-
0.072
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
native factor Xa, pH 7.5
0.089
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
recombinant factor Xa, pH 7.5
0.13
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
prothrombinase assembled with recombinant factor Xa, pH 7.5
0.17
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
prothrombinase assembled with native factor Xa, pH 7.5
0.29
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
prothrombinase assembled with mutant I16L, pH 7.5
0.49
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
prothrombinase assembled with mutant V17A, pH 7.5
1.15
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
mutant I16L, pH 7.5
1.38
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
mutant V17A, pH 7.5
0.042
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
pH 7.4, 23°C, in presence of 0.001 mM heparin
0.057
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
pH 7.4, 23°C, in absence of heparin
0.065
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
recombinant mutant R150A, 22°C
0.068
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
recombinant mutant R143A, 22°C
0.069
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
recombinant mutant K147A, 22°C
0.083
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
recombinant wild-type enzyme, 22°C
0.09
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
native wild-type enzyme, 22°C
0.122
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
recombinant mutant R154A, 22°C
0.0052
prethrombin-1
-
prothrombinase assembled with recombinant factor Xa, pH 7.5
-
0.0054
prethrombin-1
-
prothrombinase assembled with native factor Xa, pH 7.5
-
0.0057
prethrombin-1
-
prothrombinase assembled with mutant I16L, pH 7.5
-
0.0069
prethrombin-1
-
prothrombinase assembled with mutant V17A, pH 7.5
-
0.000018
prothrombin
-
recombinant mutant K147A, 22°C
-
0.000019
prothrombin
-
recombinant mutant R143A, 22°C
-
0.000023
prothrombin
-
recombinant mutant R154A, 22°C
-
0.000025
prothrombin
-
recombinant mutant R150A, 22°C
-
0.000027
prothrombin
-
recombinant wild-type enzyme, 22°C
-
0.000029
prothrombin
-
native wild-type enzyme, 22°C
-
0.000045
prothrombin
-
pH 7.4, 25°C, mutant enzyme E36K/E37K/E39K
-
0.000057
prothrombin
-
pH 7.4, 25°C, mutant enzyme E36Q/E37Q/E39Q
-
0.000079
prothrombin
-
pH 7.7, 37°C
-
0.000086
prothrombin
-
pH 7.4, 25°C, wild-type enzyme
-
0.000111
prothrombin
-
pH 7.4, 25°C, mutant enzyme E74K/E76K/E77K
-
0.000129
prothrombin
-
pH 7.4, 25°C, mutant enzyme E74Q, E76Q/E77Q
-
0.00023
prothrombin
-
prothrombinase complex, pH 7.4, 22°C
-
0.00026
prothrombin
-
dimer, presence of 5 mM Ca2+
-
0.00031
prothrombin
-
prothrombinase assembled with mutant I16L, pH 7.5
-
0.00035
prothrombin
-
prothrombinase assembled with recombinant factor Xa, pH 7.5
-
0.0004
prothrombin
-
monomer, presence of 5 mM Ca2+
-
0.00041
prothrombin
-
pH 7.4, 22°C, in presence of 20 mM factor Va
-
0.00042
prothrombin
-
prothrombinase assembled with native factor Xa, pH 7.5
-
0.00047
prothrombin
-
prothrombinase assembled with mutant V17A, pH 7.5
-
0.000605
prothrombin
-
monomer, presence of 3 mM Ca2+
-
0.00068
prothrombin
-
pH 7.4, 22°C, in absence of factor Va
-
0.000985
prothrombin
-
dimer, presence of 3 mM Ca2+
-
0.0072
prothrombin
-
pH 7.4, 37°C, wild-type enzyme in presence of factor Va
-
0.0083
prothrombin
-
pH 7.4, 37°C, wild-type enzyme
-
0.00036
recombinant prothrombin R155A/R284A/R271A mutant rMZ-II
-
pH 7.4, 22°C, in presence of 20 mM factor Va
-
0.00045
recombinant prothrombin R155A/R284A/R271A mutant rMZ-II
-
pH 7.4, 22°C, in absence of factor Va
-
0.00039
recombinant prothrombin R155A/R284A/R320A mutant rP2-II
-
pH 7.4, 22°C, in presence of 20 mM factor Va
-
0.0005
recombinant prothrombin R155A/R284A/R320A mutant rP2-II
-
pH 7.4, 22°C, in absence of factor Va
-
0.133
S2222
-
factor Xa, pH 7.5, 22°C
0.318
S2222
-
pH 7.4, 25°C, wild-type enzyme
0.333
S2222
-
pH 7.4, 25°C, mutant enzyme E36Q/E37Q/E39Q
0.339
S2222
-
pH 7.4, 25°C, mutant enzyme E36K/E37K/E39K
0.529
S2222
-
pH 7.4, 25°C, mutant enzyme E74Q, E76Q/E77Q
0.739
S2222
-
pH 7.4, 25°C, mutant enzyme E74K/E76K/E77K
0.052
S2765
-
pH 7.4, 25°C, mutant enzyme E36Q/E37Q/E39Q
0.059
S2765
-
pH 7.4, 25°C, mutant enzyme E36K/E37K/E39K
0.061
S2765
-
pH 7.4, 25°C, wild-type enzyme
0.094
S2765
-
pH 7.4, 25°C, mutant enzyme E74Q, E76Q/E77Q
0.112
S2765
-
pH 7.4, 25°C, mutant enzyme E74K/E76K/E77K
0.097
tosyl-glycyl-prolyl-D-arginine-4-nitroanilide
-
pH 7.4, 23°C, in presence of 0.001 mM heparin
0.206
tosyl-glycyl-prolyl-D-arginine-4-nitroanilide
-
pH 7.4, 23°C, in absence of heparin
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
binding kinetics of heparin and other glycosaminoglycans to the active site-blocked enzyme
-
additional information
additional information
-
kinetics, dissociation constants
-
additional information
additional information
-
steady state kinetics
-
additional information
additional information
-
substrate specificity for amino acid residues at positions P3, P2, P1', P2', and P3', overview
-
additional information
additional information
FXa binding kinetic parameters and effect on prothrombinase activity of Viperidae SVPLA2 isoforms, overview
-
additional information
additional information
-
FXa binding kinetic parameters and effect on prothrombinase activity of Viperidae SVPLA2 isoforms, overview
-
additional information
additional information
-
kinetics of FVIIIa inactivation
-
additional information
additional information
-
kinetics with wild-type and mutant prothrombin substrates, overview
-
additional information
additional information
-
kinetics of prothrombinase, methematical analysis, overview
-