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Literature summary for 3.4.21.6 extracted from

  • Buddai, S.K.; Toulokhonova, L.; Bergum, P.W.; Vlasuk, G.P.; Krishnaswamy, S.
    Nematode anticoagulant protein c2 reveals a site on factor Xa that is important for macromolecular substrate binding to human prothrombinase (2002), J. Biol. Chem., 277, 26689-26698.
    View publication on PubMed
Search for more literature for 3.4.21.6

Inhibitors

Inhibitors Comment Organism Structure
nematode anticoagulant protein c2 i.e. NAPc2, binds either to factor X or factor Xa, tight binding, competitive, binds to a macromolecular substrate binding, exosite site of human prothrombinase complex, recombinantly expressed in Pichia pastoris and purified, inhibition kinetics Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, dissociation constants Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required as part of thr prothrombinase enzyme complex Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prothrombin + H2O Homo sapiens
-
 thrombin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification processing of factor X to factor Xa can be performed with Russell's viper venom Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
native wild-type from plasma Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin scutelarin has similar specificity Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methoxycarbonyl-D-cyclohexylglycyl-glycyl-L-ariginine-4-nitroanilide + H2O i.e. Spectrozyme fXa, SpfXa Homo sapiens methoxycarbonyl-D-cyclohexylglycyl-glycyl-L-ariginine + 4-nitroaniline
-
 ?
additional information enzyme possesses a macromolecular substrate binding site, exosite Homo sapiens ?
-
 ?
Prethrombin 2 + H2O
-
 Homo sapiens ?
-
 ?
prothrombin + H2O
-
 Homo sapiens thrombin + ?
-
 ?

Subunits

Subunits Comment Organism
More enzyme is complexed in the prothrombinase complex with factor Va, Ca2+, and phospholipids or membrane, respectively Homo sapiens

Synonyms

Synonyms Comment Organism
factor Xa
-
 Homo sapiens
More the enzyme belongs to the peptidase family S1, i.e. trypsin family Homo sapiens
Xa
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens