Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Glycosaminoglycans | bind with higher affinity to factor Xa in presence of Ca2+ than in its absence, while the binding to the Gla-domainless factor Xa shows similar Km-values in absence or presence of Ca2+, binding induces conformational changes in the active site of factor Xa, glycosaminoglycans influence the ability of factor Xa to cleave chromogenic substrates and reduce its factor VII cleaving activity, exosites on the surface of factor Xa are perturbed, the activation effect increass with increasing number of sulfate residues | Homo sapiens | |
heparin | binds to a Ca2+-dependent binding site, reduces Km and kcat for chromogenic substrates dependent on Ca2+ | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | binding kinetics of heparin and other glycosaminoglycans to the active site-blocked enzyme | Homo sapiens | |
0.042 | - |
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide | pH 7.4, 23°C, in presence of 0.001 mM heparin | Homo sapiens | |
0.057 | - |
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide | pH 7.4, 23°C, in absence of heparin | Homo sapiens | |
0.097 | - |
tosyl-glycyl-prolyl-D-arginine-4-nitroanilide | pH 7.4, 23°C, in presence of 0.001 mM heparin | Homo sapiens | |
0.206 | - |
tosyl-glycyl-prolyl-D-arginine-4-nitroanilide | pH 7.4, 23°C, in absence of heparin | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin | scutelarin has similar specificity | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide + H2O | i.e. S-2765, synthetic chromogenic substrate | Homo sapiens | N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine + 4-nitroaniline | - |
? | |
tosyl-glycyl-prolyl-D-arginine-4-nitroanilide + H2O | i.e. Chz-TH, synthetic chromogenic substrate | Homo sapiens | tosyl-glycyl-prolyl-D-arginine + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
f.Xa | - |
Homo sapiens |
factor Xa | - |
Homo sapiens |
More | the enzyme belongs to the peptidase family S1, i.e. trypsin family | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46 | - |
tosyl-glycyl-prolyl-D-arginine-4-nitroanilide | pH 7.4, 23°C, in presence of 0.001 mM heparin | Homo sapiens | |
79 | - |
tosyl-glycyl-prolyl-D-arginine-4-nitroanilide | pH 7.4, 23°C, in absence of heparin | Homo sapiens | |
144 | - |
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide | pH 7.4, 23°C, in presence of 0.001 mM heparin | Homo sapiens | |
169 | - |
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide | pH 7.4, 23°C, in absence of heparin | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |