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3.4.21.6: coagulation factor Xa

This is an abbreviated version!
For detailed information about coagulation factor Xa, go to the full flat file.

Word Map on EC 3.4.21.6

Reaction

selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin =

Synonyms

activated blood-coagulation factor X, activated factor X, activated Stuart-Prower factor, autoprothrombin C, blood coagulation factor X, blood coagulation factor Xa, blood-coagulation factor X, activated, clotting factor Xa, coagulation factor X, coagulation factor Xa, f.Xa, factor X, factor Xa, Fgl2 protein, fX, FXa, FXaalpha/beta, hXa, More, oscutarin, plasma thromboplastin, procoagulant factor X, prothrombase, prothrombin activator, prothrombinase, Stuart factor, thrombokinase, thromboplastin, thromboplastin, plasma, trocarin, Trocarin prothrombin activator, VAP, Virus activating protease, Xa

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.6 coagulation factor Xa

Substrates Products

Substrates Products on EC 3.4.21.6 - coagulation factor Xa

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-VQFRILGDQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-VQFR + ILGDQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-VQFRSAGDQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-VQFR + SAGDQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-VQFRSLEDQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-VQFR + SLEDQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-VQFRSLTDQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-VQFR + SLTDQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-VQFRSVGDQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-VQFR + SCGDQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-VQFRTLGDQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-VQFR + TLGDQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
-
?
Abz-VMIAALPRTMFIQ-ED-dinitrophenyl + H2O
?
show the reaction diagram
-
-
-
?
activated factor VIII + H2O
factor VIII
show the reaction diagram
apomyoglobin + H2O
?
show the reaction diagram
-
maleylated sperm whale apomyoglobin. Hydrolysed at the Arg31-Leu32 bond
-
-
?
Arg-Gly-Arg-4-nitroanilide + H2O
Arg-Gly-Arg + 4-nitroaniline
show the reaction diagram
benzoyl-Ile-Glu(gamma-O-methyl)-Gly-Arg-4-nitroanilide + N-benzoyl-Ile-Glu-Gly-Arg-4-nitroanilide + H2O
benzoyl-Ile-Glu(gamma-O-methyl)-Gly-Arg + N-benzoyl-Ile-Glu-Gly-Arg + 4-nitroaniline
show the reaction diagram
benzoyl-Ile-Glu(gamma-OR)-Gly-Arg-4-nitroanilide + H2O
benzoyl-Ile-Glu(gamma-OR)-Gly-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
benzoyl-Ile-Glu(piperidineamide)-Gly-Arg-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
benzoyl-Ile-Glu-(-H/OMe)-Gly-Arg-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
Boc-L-Asp(OBzl)-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
Boc-L-Asp(OBzl)-L-Pro-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
chymotrypsin + H2O
?
show the reaction diagram
-
poor substrate
-
-
?
chymotrypsinogen + H2O
?
show the reaction diagram
-
maleylated and performate-oxidized bovine chymotrypsinogen A is hydrolyzed at the Arg230-Val231 bond and at the Arg15-Ile16 bond
-
-
?
D-cyclohexyl-Gly-L-Pro-L-Arg-4-nitroanilide + H2O
D-cyclohexyl-Gly-L-Pro-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
D-hexahydrotyrosol-L-Ala-L-Arg-4-nitroanilide + H2O
D-hexahydrotyrosol-L-Ala-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
D-hexahydrotyrosyl-L-alanyl-L-arginine-4-nitroanilide + H2O
D-hexahydrotyrosyl-L-alanyl-L-arginine + 4-nitroaniline
show the reaction diagram
-
synthetic chromogenic substrate
-
?
D-Phe-Pip-Arg-4-nitroanilide + H2O
D-Phe-Pip-Arg + 4-nitroaniline
show the reaction diagram
-
synthetic substrate S2238
-
-
?
D-phenylalanyl-L-pipecolyl-L-arginine-4-nitroanilide + H2O
D-phenylalanyl-L-pipecolyl-L-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
dimethylsulfonyl-D-norleucine-Gly-Arg-4-nitroanilide + H2O
dimethylsulfonyl-D-norleucine-Gly-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
factor VII + H2O
?
show the reaction diagram
-
cleavage of two bonds, rapid cleavage of an Arg-Ile bond and slower cleavage of an Y-Gly bond
-
-
?
factor VIII + H2O
?
show the reaction diagram
-
factor Xa proteolytically activates Factor VIII by cleaving P1 residues Arg372, Arg740, and Arg1689. Factor Xa also catalyzes inactivating cleavages that occur on a slower time scale than the activating ones
-
-
?
factor VIII + H2O
activated factor VIII + B domain
show the reaction diagram
factor X + H2O
?
show the reaction diagram
-
cleavage of three bonds
-
-
?
FPR-prothrombin + H2O
meizothrombin
show the reaction diagram
-
i.e. prothrombin specifically active site-labeled with D-Phe-Pro-Arg-CH2Cl is cleaved by prothrombinase to intermediate meizothrombin, but the reaction is then directed to formation of a different FPR-prothrombin 2 intermediate product
-
-
?
FPR-prothrombin + H2O
meizothrombin + ?
show the reaction diagram
-
i.e. prothrombin specifically active site-labeled with D-Phe-Pro-Arg-CH2Cl and AF660-labeled, is cleaved by prothrombinase to intermediate meizothrombin, but the reaction is then directed to formation of a different FPR-prothrombin 2 intermediate product
-
-
?
fragment 1.2:prethrombin-2 + H2O
thrombin
show the reaction diagram
-
intermediate of prothrombin processing to thrombin, cleavage site is Arg320
-
?
FS-2765 + H2O
?
show the reaction diagram
GPRFFL + H2O
GPR + FFL
show the reaction diagram
-
best substrate
-
?
L-Arg-Gly-Arg-4-nitroanilide + H2O
L-Arg-Gly-Arg + 4-nitroaniline
show the reaction diagram
L-Arg-Gly-L-Arg-4-nitroanilide + H2O
L-Arg-Gly-L-Arg + 4-nitroaniline
show the reaction diagram
meizothrombin + H2O
thrombin
show the reaction diagram
-
intermediate of prothrombin processing to thrombin, cleavage site is Arg271
-
?
methoxycarbonyl-D-cyclohexylglycyl-Gly-L-Arg-4-nitroanilide + H2O
methoxycarbonyl-D-cyclohexylglycyl-Gly-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
methoxycarbonyl-D-cyclohexylglycyl-glycyl-L-ariginine-4-nitroanilide + H2O
methoxycarbonyl-D-cyclohexylglycyl-glycyl-L-ariginine + 4-nitroaniline
show the reaction diagram
-
i.e. Spectrozyme fXa, SpfXa
-
?
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide + H2O
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-2-benzyloxycarbonyl-D-arginyl-L-arginine-4-nitroanilide + H2O
N-2-benzyloxycarbonyl-D-arginyl-L-arginine + 4-nitroaniline
show the reaction diagram
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide + H2O
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine + 4-nitroaniline
show the reaction diagram
N-alpha-benzyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Ile-Glu-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
N-benzoyl-Arg-4-nitroanilide + H2O
N-benzoyl-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
N-benzoyl-Ile-Glu-Gly-Arg-4-nitroanilide + H2O
N-benzoyl-Ile-Glu-Gly-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
N6-CBZ-D-Lys-L-Pro-L-Arg-4-nitroanilide + H2O
N6-CBZ-D-Lys-L-Pro-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzyloxycarbonyl-L-Lys-4-nitrophenyl ester + H2O
Nalpha-benzyloxycarbonyl-L-Lys + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
prethrombin 1 + H2O
thrombin + ?
show the reaction diagram
-
activation of prethrombin 1 is slow with cleavages at residues R320 and R271 occuring with similar rates
-
-
?
Prethrombin 2 + H2O
?
show the reaction diagram
prethrombin-1 + H2O
thrombin + ?
show the reaction diagram
-
-
-
-
?
prethrombin-2 + H2O
thrombin-2
show the reaction diagram
-
smallest zymogen form of thrombin containing only the factor Xa cleavage site 2
-
?
protease-activated receptor 1 + H2O
activated protease-activated receptor 1 + ?
show the reaction diagram
protease-activated receptor 2 + H2O
activated protease-activated receptor 2 + ?
show the reaction diagram
prothrombin + H2O
?
show the reaction diagram
prothrombin + H2O
thrombin + ?
show the reaction diagram
prothrombin + H2O
thrombin + activation peptide
show the reaction diagram
-
-
-
?
prothrombin + H2O
thrombin + propeptide of thrombin
show the reaction diagram
prothrombin + H2O
thrombin + thrombin N-terminal fragment F12
show the reaction diagram
-
at the physiological concentration of prothrombin, thrombin formation results in rapid release of the product into solution due to weak interaction between thrombin and the cleaved N-terminal F12 domain, of which fragment F1 plays an essential role in membrane binding. Thrombin itself interacts with poor affinity with the F12 domain
-
-
?
prothrombin + H2O
thrombin + thrombin propeptide
show the reaction diagram
-
-
-
-
?
prothrombin + H2O
trhombin + ?
show the reaction diagram
ProTS195A + H2O
?
show the reaction diagram
-
the mutant protein also acts as competitive inhibitor of prothrombin activation
-
-
?
recombinant prothrombin R155A/R284A/R271A mutant rMZ-II + H2O
meizothrombin
show the reaction diagram
-
cleavage site is Arg320
reaction intermediate in the reaction with wild-type prothrombin
?
recombinant prothrombin R155A/R284A/R271A/S525C mutant rMZ-II-F + H2O
meizothrombin
show the reaction diagram
-
labeled with fluorescein at Cys525, cleavage site is Arg320
reaction intermediate in the reaction with wild-type prothrombin
?
recombinant prothrombin R155A/R284A/R320A mutant rP2-II + H2O
fragment 1.2:prethrombin-2
show the reaction diagram
-
cleavage site is Arg271
reaction intermediate in the reaction with wild-type prothrombin
?
recombinant prothrombin S525C mutant + H2O
thrombin
show the reaction diagram
-
labeled with fluorescein at Cys525, cleavage sites are Arg271 and Arg320
-
?
S-2765 + H2O
Z-D-Arg-Gly-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
S2222 + H2O
?
show the reaction diagram
S2238 + H2O
?
show the reaction diagram
S2765 + H2O
?
show the reaction diagram
-
-
-
-
?
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
tosyl-glycyl-prolyl-D-arginine-4-nitroanilide + H2O
tosyl-glycyl-prolyl-D-arginine + 4-nitroaniline
show the reaction diagram
-
i.e. Chz-TH, synthetic chromogenic substrate
-
?
Trypsinogen + H2O
?
show the reaction diagram
-
maleylated performate-oxidized trypsinogen, slowly hydrolyzed at the Arg117-Val118 bond
-
-
?
Z-D-Arg-Gly-Arg-4-nitroanilide + H2O
Z-D-Arg-Gly-Arg + 4-nitroaniline
show the reaction diagram
Z-D-Arg-Gly-L-Arg-4-nitroanilide + H2O
Z-D-Arg-Gly-L-Arg + 4-nitroaniline
show the reaction diagram
additional information
?
-