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Literature summary for 3.4.21.6 extracted from

  • Rezaie, A.R.; He, X.
    Sodium binding site of factor Xa: role of sodium in the prothrombinase complex (2000), Biochemistry, 39, 1817-1825.
    View publication on PubMed
Search for more literature for 3.4.21.6

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ binding to the enzyme stabilizes the binding of Na+, required for prothrombinase complex formation Homo sapiens
K+ can partly replace Na+, less efficient Homo sapiens
Li+ can partly replace Na+, less efficient Homo sapiens
Na+ bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression by the RSV-PL4 expression/purification vector system of wild-type inactive factor X, mutant inactive factor X lacking the 4-carboxyglutamic acid-domain, Y225P mutant of factor X, factor X E2FX mutant lacking both Gla-domain and EGF1-domain, as well as of mutants D70K and E80K of mutant E2FX, expression in human 293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D70K mutation of E2FXa mutant, loss of ability to bind Ca2+, activity is retained Homo sapiens
E80K mutation of E2FXa mutant, loss of ability to bind Ca2+, activity is retained Homo sapiens
additional information mutant GDFXa shows reduced ability to differentiate between the metal ions bound and reduced amidolyic activity Homo sapiens
Y225P mutation of the GDFXa mutant, exchange of the Na+ binding site residue results in 2fold decreased stimulation by Na+ in presence or absence of Ca2+ Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prothrombin + H2O Homo sapiens acts within the prothrombinase complex thrombin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification activation of factor X and GDFX to factor Xa and GDFXa, respectively, by Russel's viper venom and human antithrombin Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type inactive factor X and mutant proteins Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin scutelarin has similar specificity Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Arg-Gly-Arg-4-nitroanilide + H2O chromogenic synthetic substrate S2765 Homo sapiens L-Arg-Gly-Arg + 4-nitroaniline
-
 ?
prothrombin + H2O
-
 Homo sapiens thrombin + ?
-
 ?
prothrombin + H2O acts within the prothrombinase complex Homo sapiens thrombin + ?
-
 ?
S2222 + H2O chromogenic synthetic peptide substrate Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More factor Xa is part of the prothrombinase complex also including e.g. factor Va Homo sapiens

Synonyms

Synonyms Comment Organism
factor Xa
-
 Homo sapiens
FXa
-
 Homo sapiens
More the enzyme belongs to the peptidase family S1, i.e. trypsin family Homo sapiens
prothrombinase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 room temperature, assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens