Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | binding to the enzyme stabilizes the binding of Na+, required for prothrombinase complex formation | Homo sapiens | |
K+ | can partly replace Na+, less efficient | Homo sapiens | |
Li+ | can partly replace Na+, less efficient | Homo sapiens | |
Na+ | bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression by the RSV-PL4 expression/purification vector system of wild-type inactive factor X, mutant inactive factor X lacking the 4-carboxyglutamic acid-domain, Y225P mutant of factor X, factor X E2FX mutant lacking both Gla-domain and EGF1-domain, as well as of mutants D70K and E80K of mutant E2FX, expression in human 293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D70K | mutation of E2FXa mutant, loss of ability to bind Ca2+, activity is retained | Homo sapiens |
E80K | mutation of E2FXa mutant, loss of ability to bind Ca2+, activity is retained | Homo sapiens |
additional information | mutant GDFXa shows reduced ability to differentiate between the metal ions bound and reduced amidolyic activity | Homo sapiens |
Y225P | mutation of the GDFXa mutant, exchange of the Na+ binding site residue results in 2fold decreased stimulation by Na+ in presence or absence of Ca2+ | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prothrombin + H2O | Homo sapiens | acts within the prothrombinase complex | thrombin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | activation of factor X and GDFX to factor Xa and GDFXa, respectively, by Russel's viper venom and human antithrombin | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant wild-type inactive factor X and mutant proteins | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin | scutelarin has similar specificity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg-Gly-Arg-4-nitroanilide + H2O | chromogenic synthetic substrate S2765 | Homo sapiens | L-Arg-Gly-Arg + 4-nitroaniline | - |
? | |
prothrombin + H2O | - |
Homo sapiens | thrombin + ? | - |
? | |
prothrombin + H2O | acts within the prothrombinase complex | Homo sapiens | thrombin + ? | - |
? | |
S2222 + H2O | chromogenic synthetic peptide substrate | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | factor Xa is part of the prothrombinase complex also including e.g. factor Va | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
factor Xa | - |
Homo sapiens |
FXa | - |
Homo sapiens |
More | the enzyme belongs to the peptidase family S1, i.e. trypsin family | Homo sapiens |
prothrombinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
room temperature, assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |