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27.1 - 154.4
Arg-Gly-Arg-4-nitroanilide
145 - 149
benzoyl-Ile-Glu(piperidineamide)-Gly-Arg-p-nitroanilide
140 - 146
benzoyl-Ile-Glu-(-H/OMe)-Gly-Arg-p-nitroanilide
0.0057 - 114
fragment 1.2:prethrombin-2
-
6.39 - 150
meizothrombin
-
33 - 210
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
1.4 - 411
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
2.17
Nalpha-benzyloxycarbonyl-L-Lys-p-nitrophenyl ester
-
-
6.5 - 18.1
prethrombin-1
-
20.5
prethrombin-2
-
prothrombinase complex, pH 7.4, 22°C
-
0.0000000018 - 108
prothrombin
-
0.0068 - 110
recombinant prothrombin R155A/R284A/R271A mutant rMZ-II
-
0.324 - 114
recombinant prothrombin R155A/R284A/R320A mutant rP2-II
-
21
S-2765
-
wild type enzyme, at pH 7.5 and 25°C
46 - 79
tosyl-glycyl-prolyl-D-arginine-4-nitroanilide
additional information
meizothrombin
-
27.1
Arg-Gly-Arg-4-nitroanilide
-
mutant E217A, pH 7.5
57.2
Arg-Gly-Arg-4-nitroanilide
-
mutant E217A, pH 7.5, presence of 0.5 M NaCl
94.3
Arg-Gly-Arg-4-nitroanilide
-
mutant E217Q, pH 7.5
99.2
Arg-Gly-Arg-4-nitroanilide
-
mutant K222A, pH 7.5
125.3
Arg-Gly-Arg-4-nitroanilide
-
wild-type, pH 7.5
151.7
Arg-Gly-Arg-4-nitroanilide
-
mutant E217Q, pH 7.5, presence of 0.5 M NaCl
154.4
Arg-Gly-Arg-4-nitroanilide
-
mutant R221A, pH 7.5
145
benzoyl-Ile-Glu(piperidineamide)-Gly-Arg-p-nitroanilide
-
-
145
benzoyl-Ile-Glu(piperidineamide)-Gly-Arg-p-nitroanilide
-
native enzyme
149
benzoyl-Ile-Glu(piperidineamide)-Gly-Arg-p-nitroanilide
-
enzyme from which the residues 1-44 of the light chain have been removed by chymotryptic proteolysis
140
benzoyl-Ile-Glu-(-H/OMe)-Gly-Arg-p-nitroanilide
-
enzyme from which the residues 1-44 of the light chain have been removed by chymotryptic proteolysis
146
benzoyl-Ile-Glu-(-H/OMe)-Gly-Arg-p-nitroanilide
-
-
146
benzoyl-Ile-Glu-(-H/OMe)-Gly-Arg-p-nitroanilide
-
native enzyme
0.0057
fragment 1.2:prethrombin-2
-
pH 7.4, 22°C, in absence of factor Va
-
114
fragment 1.2:prethrombin-2
-
pH 7.4, 22°C, in presence of 20 mM factor Va
-
6.39
meizothrombin
-
pH 7.4, 22°C, in absence of factor Va
-
150
meizothrombin
-
pH 7.4, 22°C, in presence of 20 mM factor Va
-
33
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
prothrombinase assembled with mutant I16L, pH 7.5
58
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
mutant I16L, pH 7.5
72
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
prothrombinase assembled with native factor Xa, pH 7.5
73
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
mutant V17A, pH 7.5
91
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
prothrombinase assembled with mutant V17A, pH 7.5
140
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
native factor Xa, pH 7.5
140
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
prothrombinase assembled with recombinant factor Xa, pH 7.5
210
methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide
-
recombinant factor Xa, pH 7.5
1.4
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
recombinant mutant R143A, 22°C
21
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
recombinant mutant R150A, 22°C
55
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
recombinant mutant K147A, 22°C
98.6
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
recombinant mutant R143A, 22°C
121
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
native wild-type enzyme, 22°C
126.5
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
recombinant wild-type enzyme, 22°C
127
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
recombinant wild-type enzyme, 22°C
144
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
pH 7.4, 23°C, in presence of 0.001 mM heparin
169
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
pH 7.4, 23°C, in absence of heparin
411
N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide
-
recombinant mutant R154A, 22°C
6.5
prethrombin-1
-
prothrombinase assembled with mutant V17A, pH 7.5
-
6.6
prethrombin-1
-
prothrombinase assembled with mutant I16L, pH 7.5
-
17.5
prethrombin-1
-
prothrombinase assembled with recombinant factor Xa, pH 7.5
-
18.1
prethrombin-1
-
prothrombinase assembled with native factor Xa, pH 7.5
-
0.0000000018
prothrombin
-
dimer, presence of 3 mM Ca2+
-
0.000000002
prothrombin
-
dimer, presence of 5 mM Ca2+
-
0.0029
prothrombin
-
monomer, presence of 3 mM Ca2+
-
0.004
prothrombin
-
monomer, presence of 5 mM Ca2+
-
0.0045
prothrombin
-
pH 7.4, 22°C, in absence of factor Va
-
0.6
prothrombin
-
pH 7.4, 25°C, mutant enzyme E36K/E37K/E39K, activated by factor Va
-
0.6
prothrombin
-
pH 7.4, 25°C, mutant enzyme E36Q/E37Q/E39Q, activated by factor Va
-
5.7
prothrombin
-
pH 7.4, 25°C, wild-type enzyme, activated by factor Va
-
7.3
prothrombin
-
pH 7.4, 25°C, mutant enzyme E74K/E76K/E77K, activated by factor Va
-
10.3
prothrombin
-
prothrombinase assembled with mutant I16L, pH 7.5
-
10.5
prothrombin
-
pH 7.4, 25°C, mutant enzyme E74Q, E76Q/E77Q, activated by factor Va
-
14.8
prothrombin
-
prothrombinase assembled with mutant V17A, pH 7.5
-
16.9
prothrombin
-
recombinant mutant R154A, 22°C
-
17.2
prothrombin
-
recombinant mutant R143A, 22°C
-
19
prothrombin
-
recombinant mutant K147A, 22°C
-
19.6
prothrombin
-
prothrombinase complex, pH 7.4, 22°C
-
21.2
prothrombin
-
recombinant mutant R150A, 22°C
-
23
prothrombin
-
recombinant wild-type enzyme, 22°C
-
26.5
prothrombin
-
native wild-type enzyme, 22°C
-
32.3
prothrombin
-
prothrombinase assembled with recombinant factor Xa, pH 7.5
-
40.4
prothrombin
-
prothrombinase assembled with native factor Xa, pH 7.5
-
81
prothrombin
-
pH 7.7, 37°C
-
96
prothrombin
-
wild-type substrate, for cleavage at Arg320
-
108
prothrombin
-
pH 7.4, 22°C, in presence of 20 mM factor Va
-
0.0068
recombinant prothrombin R155A/R284A/R271A mutant rMZ-II
-
pH 7.4, 22°C, in absence of factor Va
-
110
recombinant prothrombin R155A/R284A/R271A mutant rMZ-II
-
pH 7.4, 22°C, in presence of 20 mM factor Va
-
0.324
recombinant prothrombin R155A/R284A/R320A mutant rP2-II
-
pH 7.4, 22°C, in absence of factor Va
-
114
recombinant prothrombin R155A/R284A/R320A mutant rP2-II
-
pH 7.4, 22°C, in presence of 20 mM factor Va
-
139
S2222
-
pH 7.4, 25°C, mutant enzyme E74K/E76K/E77K
148
S2222
-
pH 7.4, 25°C, mutant enzyme E74Q, E76Q/E77Q
196
S2222
-
pH 7.4, 25°C, mutant enzyme E36K/E37K/E39K
200
S2222
-
pH 7.4, 25°C, mutant enzyme E36Q/E37Q/E39Q
211
S2222
-
pH 7.4, 25°C, wild-type enzyme
214
S2765
-
pH 7.4, 25°C, mutant enzyme E36Q/E37Q/E39Q
215
S2765
-
pH 7.4, 25°C, mutant enzyme E36K/E37K/E39K
254
S2765
-
pH 7.4, 25°C, wild-type enzyme
269
S2765
-
pH 7.4, 25°C, mutant enzyme E74K/E76K/E77K
270
S2765
-
pH 7.4, 25°C, mutant enzyme E74Q, E76Q/E77Q
46
tosyl-glycyl-prolyl-D-arginine-4-nitroanilide
-
pH 7.4, 23°C, in presence of 0.001 mM heparin
79
tosyl-glycyl-prolyl-D-arginine-4-nitroanilide
-
pH 7.4, 23°C, in absence of heparin
additional information
meizothrombin
-
pH 7.4, 22°C, in absence of factor Va
-
additional information
additional information
-
kcat values of prothrombinase assembled with mutant cofactor factor Va D334K/Y335F or D334A/Y335A is reduced. Prothrombinase assembled with quadruple mutant molecules E323Y/E330Y/D334K/Y335F or E323Y/E330Y/D334A/Y335A displays a second-order rate constant up to 400fold lower than the value obtained with wild-type factor Va
-
additional information
prothrombin
-
and monomer. Dimer is about 1000000fold less active toward prothrombin than the monomer, mainly due to a substantial decrease in kcat. Dimerization results in substantial change in tertiary or quarternary structure with a concomitant decrease in alpha-helix
-