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3.2.1.B1: extracellular agarase

This is an abbreviated version!
For detailed information about extracellular agarase, go to the full flat file.

Word Map on EC 3.2.1.B1

Reaction

hydrolysis of 1,3-beta-D-galactosidic linkages in agarose, giving the octamer as the predominant product =

Synonyms

Aga21, AgaA, AgaA7, AgaB, AgaB1, AgaC, AgaG1, agaM1, agarase AG-b, agarase-a, agarase-b, beta-agarase, beta-agarase AgaB, endo-acting beta-agarase, endo-type beta-agarase, exo-beta-agarase, extracellular beta-agarase, NifU, YM01-3

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.B1 extracellular agarase

Temperature Range

Temperature Range on EC 3.2.1.B1 - extracellular agarase

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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 30
-
the enzyme maintains 85% of the maximum activity at 10°C
10 - 60
-
about 30% activity at 10°C, about 40% activity at 20°C, about 70% activity at 30°C, 100% activity at 40°C, about 50% activity at 50°C, about 20% activity at 60°C
20 - 50
25 - 45
-
about 75% activity 25°C, about 90% activity 30°C, 100% activity at 35°C, about 62% activity 40°C, about 58% activity 45°C
25 - 55
the enzyme exhibits high activity at temperatures ranging between 25 and 45°C. The enzyme can sustain its enzymatic activity up to 90% of its peak activity at 45°C, while the relative activities rapidly decline to 20 and 3% at 50 and 55°C, respectively
25 - 70
-
25°C: about 55% of maximal activity, 70°C: about 65% of maximal activity, agarase AG-b
30 - 50
the enzyme retains more than 80% of its maximal activity within a temperature range of 30°C to 50°C
35 - 50
37 - 50
-
the enzyme exhibits about 80%, 98.6% and 87.6% at 37°C, 40°C and 50°C, respectively
40 - 50
40 - 60
50
-
at 50°C, isoform agarase-a has 35.8% of its optimal activity
50 - 70
-
at 60 °C, the enzyme activity is 75% of the maximum activity at 50°C, and enzyme activity declines sharply at 70°C
50 - 80
-
the enzyme activity consistently increases from 55°C to 70°C with optimum activity at 70°C, and an obvious decrease is observed when the agarase is incubated at temperatures above 80°C