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Literature summary for 3.2.1.B1 extracted from
- Purification and characterization of two agarases from Agarivorans albus OAY02 (2014), Process Biochem., 49, 905-912.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | at 2 mM, isoforms agarase-a and agarase-b show 117.47% and 113.44% activity, respectively | Agarivorans albus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Agarivorans albus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | at 1 mM, isoforms agarase-a and agarase-b show 81.31% and 91.81% residual activity, respectively | Agarivorans albus | |
| Cu2+ | at 1 mM, isoforms agarase-a and agarase-b show 72.2% and 64.54% residual activity, respectively | Agarivorans albus | |
| EDTA | at 2 mM, isoforms agarase-a shows 71.32% residual activity | Agarivorans albus | |
| Fe2+ | at 1 mM, isoform agarase-b shows 86.36% residual activity | Agarivorans albus | |
| Mn2+ | at 1 mM, isoforms agarase-a and agarase-b show 86.14% and 62.72% residual activity, respectively | Agarivorans albus | |
| additional information | isoforms agarase-a and agarase-b are not inhibited by urea and SDS | Agarivorans albus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Agarivorans albus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | at 1 mM isoform agarase-a shows 109.95% activity | Agarivorans albus | |
| additional information | isoforms agarase-a and agarase-b are not influenced by Mg2+ and Fe3+ | Agarivorans albus | |
| Na+ | at 1 mM isoform agarase-b show 111.8% activity | Agarivorans albus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, isoform agarase-a, SDS-PAGE | Agarivorans albus |
| 107000 | - |
x * 107000, isoform agarase-b, SDS-PAGE | Agarivorans albus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | Agarivorans albus | - |
neoagarobiose + neoagarotetraose | products of isoform agarase-b | ? | |
| agarose + H2O | Agarivorans albus | - |
neoagarobiose + neoagarotetraose + neoagarohexaose | products of isoform agarase-a | ? | |
| agarose + H2O | Agarivorans albus OAY02 | - |
neoagarobiose + neoagarotetraose | products of isoform agarase-b | ? | |
| agarose + H2O | Agarivorans albus OAY02 | - |
neoagarobiose + neoagarotetraose + neoagarohexaose | products of isoform agarase-a | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agarivorans albus | - |
- |
- |
| Agarivorans albus OAY02 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE column chromatography, and Q-Sepharose column chromatography | Agarivorans albus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.26 | - |
crude extract, at pH 9.0 and 40°C | Agarivorans albus |
| 942 | - |
isoform agarase-a, after 107fold purification, at pH 9.0 and 40°C | Agarivorans albus |
| 1338 | - |
isoform agarase-b, after 52fold purification, at pH 9.0 and 40°C | Agarivorans albus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | - |
Agarivorans albus | neoagarobiose + neoagarotetraose | products of isoform agarase-b | ? | |
| agarose + H2O | - |
Agarivorans albus OAY02 | neoagarobiose + neoagarotetraose | products of isoform agarase-b | ? | |
| agarose + H2O | - |
Agarivorans albus | neoagarobiose + neoagarotetraose + neoagarohexaose | products of isoform agarase-a | ? | |
| agarose + H2O | - |
Agarivorans albus OAY02 | neoagarobiose + neoagarotetraose + neoagarohexaose | products of isoform agarase-a | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 107000, isoform agarase-b, SDS-PAGE | Agarivorans albus |
| ? | x * 50000, isoform agarase-a, SDS-PAGE | Agarivorans albus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| agarase-a | isoform | Agarivorans albus |
| agarase-b | isoform | Agarivorans albus |
| beta-agarase | - |
Agarivorans albus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
isoform agarase-a | Agarivorans albus |
| 50 | - |
isoform agarase-b | Agarivorans albus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
at 50°C, isoform agarase-a has 35.8% of its optimal activity | Agarivorans albus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | isoform garase-a is stable at 30°C, while agarase-b is stable at 50°C. Agarase-a shows residual activity remaining above 70% after 60 min at 2030°C. After incubation at 40°C for 60 min, the remaining activity decreases to 28%. Agarase-b shows more than 75% of the maximal activity at 50°C | Agarivorans albus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Agarivorans albus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | isoform agarase-a is stable over a wide pH range of 4.0-10.0 with more than 60% activity. Isoform agarase-b retains more than 60% activity over a pH range of 5.0-10.0 | Agarivorans albus |
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