Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 85% residual activity at 1 mM | Microbulbifer sp. | |
Co2+ | complete inhibition at 1 mM | Microbulbifer sp. | |
Cu2+ | complete inhibition at 1 mM | Microbulbifer sp. | |
EDTA | 46% residual activity at 1 mM | Microbulbifer sp. | |
Fe2+ | complete inhibition at 1 mM | Microbulbifer sp. | |
Hg2+ | complete inhibition at 1 mM | Microbulbifer sp. | |
Na+ | 60% residual activity at 1 mM, 72% residual activity at 10 mM, 91% residual activity at 100 mM, 83% residual activity at 1 M, complete inhibition at 3 M | Microbulbifer sp. | |
Zn2+ | complete inhibition at 1 mM | Microbulbifer sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Microbulbifer sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 123% activity at 500 mM | Microbulbifer sp. | |
Mg2+ | 112% activity at 500 mM | Microbulbifer sp. | |
Na+ | 145% activity at 500 mM | Microbulbifer sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
x * 59000, SDS-PAGE | Microbulbifer sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbulbifer sp. | - |
- |
- |
Microbulbifer sp. CMC-5 (MTCC 9889) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ultrafiltration, DEAE-Sepharose column chromatography, and Sephacryl S-300HR gel filtration | Microbulbifer sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
culture supernatant, at pH 7.0 and 30°C | Microbulbifer sp. |
4.22 | - |
after 103.5fold purification, at pH 7.0 and 30°C | Microbulbifer sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | the purified enzyme is an endo-type beta-agarase able to hydrolyze the beta-1,4 glycosidic linkages of agarose, releasing neoagarotetraose and neoagarohexaose | Microbulbifer sp. | neoagarotetraose + neoagarohexaose | major end products | ? | |
alginate + H2O | - |
Microbulbifer sp. | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Microbulbifer sp. | ? | - |
? | |
carrageenan + H2O | - |
Microbulbifer sp. | ? | - |
? | |
chitin + H2O | - |
Microbulbifer sp. | ? | - |
? | |
xylan + H2O | - |
Microbulbifer sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59000, SDS-PAGE | Microbulbifer sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Microbulbifer sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | - |
Microbulbifer sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | the agarase retains 62% of its activity after incubating at 50°C for 30 min. Further, 45% of the agarase activity is still retained at 60°C. The agarase activity is completely abolished at 70°C | Microbulbifer sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Microbulbifer sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Microbulbifer sp. |