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Literature summary for 3.2.1.B1 extracted from

  • Jonnadula, R.; Ghadi, S.
    Purification and characterization of beta-agarase from seaweed decomposing bacterium Microbulbifer sp. strain CMC-5 (2011), Biotechnol. Bioprocess Eng., 16, 513-519.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 85% residual activity at 1 mM Microbulbifer sp.
Co2+ complete inhibition at 1 mM Microbulbifer sp.
Cu2+ complete inhibition at 1 mM Microbulbifer sp.
EDTA 46% residual activity at 1 mM Microbulbifer sp.
Fe2+ complete inhibition at 1 mM Microbulbifer sp.
Hg2+ complete inhibition at 1 mM Microbulbifer sp.
Na+ 60% residual activity at 1 mM, 72% residual activity at 10 mM, 91% residual activity at 100 mM, 83% residual activity at 1 M, complete inhibition at 3 M Microbulbifer sp.
Zn2+ complete inhibition at 1 mM Microbulbifer sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Microbulbifer sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 123% activity at 500 mM Microbulbifer sp.
Mg2+ 112% activity at 500 mM Microbulbifer sp.
Na+ 145% activity at 500 mM Microbulbifer sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
x * 59000, SDS-PAGE Microbulbifer sp.

Organism

Organism UniProt Comment Textmining
Microbulbifer sp.
-
-
-
Microbulbifer sp. CMC-5 (MTCC 9889)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration, DEAE-Sepharose column chromatography, and Sephacryl S-300HR gel filtration Microbulbifer sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
culture supernatant, at pH 7.0 and 30°C Microbulbifer sp.
4.22
-
after 103.5fold purification, at pH 7.0 and 30°C Microbulbifer sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O the purified enzyme is an endo-type beta-agarase able to hydrolyze the beta-1,4 glycosidic linkages of agarose, releasing neoagarotetraose and neoagarohexaose Microbulbifer sp. neoagarotetraose + neoagarohexaose major end products ?
alginate + H2O
-
Microbulbifer sp. ?
-
?
carboxymethyl cellulose + H2O
-
Microbulbifer sp. ?
-
?
carrageenan + H2O
-
Microbulbifer sp. ?
-
?
chitin + H2O
-
Microbulbifer sp. ?
-
?
xylan + H2O
-
Microbulbifer sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 59000, SDS-PAGE Microbulbifer sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Microbulbifer sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60
-
Microbulbifer sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 the agarase retains 62% of its activity after incubating at 50°C for 30 min. Further, 45% of the agarase activity is still retained at 60°C. The agarase activity is completely abolished at 70°C Microbulbifer sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Microbulbifer sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
Microbulbifer sp.