3.2.1.B1: extracellular agarase

This is an abbreviated version!
For detailed information about extracellular agarase, go to the full flat file.

Reaction

hydrolysis of 1,3-beta-D-galactosidic linkages in agarose, giving the octamer as the predominant product =

Synonyms

Aga21, AgaA, AgaA7, AgaB, AgaB1, AgaC, AgaG1, agaM1, agarase AG-b, agarase-a, agarase-b, beta-agarase, beta-agarase AgaB, endo-acting beta-agarase, endo-type beta-agarase, exo-beta-agarase, extracellular beta-agarase, NifU, YM01-3

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.B1 extracellular agarase

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Results	in table
11	Activating Compound
7	AA Sequence
1	Application
12	Cloned(Commentary)
3	Expression
3	General Stability
1	IC50 Value
167	Inhibitors
1	kcat/KM [mM/s]
4	KM Value [mM]
40	Localization
47	Metals/Ions
24	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
50	Organism
27	pH Optimum
17	pH Range
17	pH Stability
4	pI Value
1	Posttranslational Modification
5	Protein Variants
22	Purification (Commentary)
2	Reaction
48	Reference
1	Renatured (Commentary)
10	Source Tissue
15	Specific Activity [micromol/min/mg]
1	Storage Stability
88	Substrates and Products (Substrate)
47	Subunits
49	Synonyms
1	Systematic Name
29	Temperature Optimum [°C]
19	Temperature Range [°C]
27	Temperature Stability [°C]
6	Turnover Number [1/s]

pH Stability

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of the Enzyme Summary Page.

pH Stability on EC 3.2.1.B1 - extracellular agarase

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

12 - 13

Pseudomonas sp.

-

the enzyme retains about 62% of its activity at pH 12.0 and almost 50% at pH 13.0 after a pre-incubation of 1 h

731420, 750000

3 - 11

Agarivorans sp.

C9WIW7

the enzyme still retains more than 95% activity after incubation at pH 3.0-11.0 for 1 h

705482

4 - 10

2 entries

4 - 8

2 entries

4 - 9

2 entries

5 - 11

Stenotrophomonas sp. NTa

-

the enzyme exhibits good stability in pH ranging from 5.0 to 11.0, retaining more than 65% of its original activity after 1 h incubation

750831

5 - 8

3 entries

5 - 9

Agarivorans albus

A8W969

the enzyme exhibits more than 80% of its maximal activity over a pH range of 5.0 to 9.0 after 1 h incubation and shows more than 40% of maximal activity at pH values below 4.0 and above 11.0

752013

5.7 - 10.6

Pseudoalteromonas sp.

A1A3Y9

-

681563

6 - 9

Microbulbifer sp. Q7

-

the enzyme is stable between pH 6.0 and pH 9.0 for 1 h

749549

6.5 - 8.5

Pseudoalteromonas sp. NJ21

-

the enzyme is stable (more than 70% relative activity) in buffers with a pH range of 6.5-8.5

750214

4 - 10

Agarivorans albus

-

isoform agarase-a is stable over a wide pH range of 4.0-10.0 with more than 60% activity. Isoform agarase-b retains more than 60% activity over a pH range of 5.0-10.0

732848

4 - 10

Vibrio sp.

-

4°C, 6 h, stable in the pH range of 4.0–10.0, agarase AG-b

699362

4 - 8

Acinetobacter junii

-

the purified enzyme is most stable at pH 7.0-8.0 with more than 98% residual activity after 24 h. The residual activity of enzyme reduces to less than 50% when incubated for 24 h at pH 4.0

749633

4 - 8

Vibrio sp.

Q0KKW9

-

677674

4 - 9

Catenovulum agarivorans

T1WFD0

the enzyme retains more than 80% of activity after incubation at a wide range of 4.0–9.0 for 12 h at 4°C

732434

4 - 9

Microbulbifer sp. BN3

-

the enzyme is stable between pH 4.0-9.0 and retains over 70% activity after 1 h incubation at pH 6.0 and 50 °C

750807

5 - 8

Bacillus sp. (in: Bacteria)

-

more than 60% of the residual activity is retained in the pH range of 5.8-8.0

732328

5 - 8

Rhodococcus sp.

-

the enzyme is stable in the pH range from pH 5.0 to 8.0 with more than 87% of the residual activity retained after 30 min

731214

5 - 8

Rhodococcus sp. Q5

-

the enzyme is stable for 30 min in the pH range from pH 5.0 to 8.0, and more than 87% of the residual activity is retained

749732