Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.B1: extracellular agarase

This is an abbreviated version!
For detailed information about extracellular agarase, go to the full flat file.

Word Map on EC 3.2.1.B1

Reaction

hydrolysis of 1,3-beta-D-galactosidic linkages in agarose, giving the octamer as the predominant product =

Synonyms

Aga21, AgaA, AgaA7, AgaB, AgaB1, AgaC, AgaG1, agaM1, agarase AG-b, agarase-a, agarase-b, beta-agarase, beta-agarase AgaB, endo-acting beta-agarase, endo-type beta-agarase, exo-beta-agarase, extracellular beta-agarase, NifU, YM01-3

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.B1 extracellular agarase

Temperature Stability

Temperature Stability on EC 3.2.1.B1 - extracellular agarase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 40
10 - 50
-
up to 80% thermostability of the enzyme is retained at 10°C and 30 °C for 180 min. When the temperature is increased to 50°C for 30 min, the enzyme relative activity is decreased to 10-20%. Nevertheless, the enzyme is fairly stable (almost 70% relative activity) at 40°C for 30 min
20 - 50
-
isoform garase-a is stable at 30°C, while agarase-b is stable at 50°C. Agarase-a shows residual activity remaining above 70% after 60 min at 20–30°C. After incubation at 40°C for 60 min, the remaining activity decreases to 28%. Agarase-b shows more than 75% of the maximal activity at 50°C
20 - 60
-
the enzyme is relatively stable at 20°C and 30°C after incubation for 1 h. Incubation at higher temperatures results in enzyme activity loss. The enzyme retains 64% and 46% of residual activities after incubation at 40°C and 50°C for 1 h, individually. It has no activity at 60°C for 30 min
20 - 80
100% activity between 20 and40°C, about 50% activity at 50°C, about 10% activity between 60-80°C (after incubation for 1 h)
30 - 40
the residual activity is above 79% at 30 and 35°C. The half-life is 56 min at 40°C
30 - 50
-
the enzyme is very stable after 30 min incubation at 30-50°C. At 60°C, the enzyme retains 40% activity
35 - 50
the enzyme is thermostable at temperatures from 35-50°C with more than 80% activity remaining after 30 min incubation
37 - 45
-
the enzyme retains 90.12% activity at 37°C after 2 h, more than 55% activity at 40 and 42°C after 1 h, and 36.16% activity at 45°C after 1 h
40
-
half-life of mutant S2 is 350 min, which was 18.4fold longer than that of wild-type AgaB
40 - 45
the enzyme is stable up to 40°C (100%), and retains more than 70% of its initial activity at 45°C after heat treatment for 30 min. The enzyme stability dramatically declines at 50°C to 19%
40 - 80
-
91.9% and 84.5% of activity are retained after pre-incubation at 40°C and 50°C for 2 h, respectively. 62.5% of the enzyme activity is lost after pre-incubation at 60°C for 2 h, and the enzyme activity is completely lost after pre-incubation at 70°C and 80°C
45
-
30 min, about 10% loss of activity, agarase AG-b
49
-
melting temperature of wild-type enzyme is 49.2°C
50
-
the enzyme is stable up to 1 h at 50°C
50 - 70
-
the agarase retains 62% of its activity after incubating at 50°C for 30 min. Further, 45% of the agarase activity is still retained at 60°C. The agarase activity is completely abolished at 70°C
50 - 80
the enzyme retains more than 80% agarolytic activity after being kept in 50°C for 1 h, 13% residual activity after incubation in 80°C for 1 h, and 12% residual activity if boiled for 5 min
54
-
melting temperature of mutant enzyme S2 is 53.8°C
55
-
30 min, about 20% loss of activity, agarase AG-b
65
-
30 min, about 50% loss of activity, agarase AG-b
70
-
30 min, about 90% loss of activity, agarase AG-b
80
-
more than 50% activity is retained at 80°C for 15 min