3.2.1.B1: extracellular agarase

This is an abbreviated version!
For detailed information about extracellular agarase, go to the full flat file.

Word Map on EC 3.2.1.B1

3.2.1.B1

neoagarotetraose

neoagarohexaose

agarolytic

neoagarobiose

agarases

coelicolor

pseudoalteromonas

endo-type

lividans

agar-degrading

atlantica

cytophaga

neoagaro-oligosaccharides

gracilaria

agarivorans

neoagarooctaose

alteromonas

synthesis

Reaction

hydrolysis of 1,3-beta-D-galactosidic linkages in agarose, giving the octamer as the predominant product =

Synonyms

Aga21, AgaA, AgaA7, AgaB, AgaB1, AgaC, AgaG1, agaM1, agarase AG-b, agarase-a, agarase-b, beta-agarase, beta-agarase AgaB, endo-acting beta-agarase, endo-type beta-agarase, exo-beta-agarase, extracellular beta-agarase, NifU, YM01-3

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.B1 extracellular agarase

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Results	in table
11	Activating Compound
7	AA Sequence
1	Application
12	Cloned(Commentary)
3	Expression
3	General Stability
1	IC50 Value
167	Inhibitors
1	kcat/KM [mM/s]
4	KM Value [mM]
40	Localization
47	Metals/Ions
24	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
50	Organism
27	pH Optimum
17	pH Range
17	pH Stability
4	pI Value
1	Posttranslational Modification
5	Protein Variants
22	Purification (Commentary)
2	Reaction
48	Reference
1	Renatured (Commentary)
10	Source Tissue
15	Specific Activity [micromol/min/mg]
1	Storage Stability
88	Substrates and Products (Substrate)
47	Subunits
49	Synonyms
1	Systematic Name
29	Temperature Optimum [°C]
19	Temperature Range [°C]
27	Temperature Stability [°C]
6	Turnover Number [1/s]

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Cloned on EC 3.2.1.B1 - extracellular agarase

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expressed in Escherichia coli BL21 (DE3) pLysS cells

Alteromonas sp.

U3PAU3

731208

expressed in Escherichia coli BL21(DE3) cells

5 entries

expressed in Escherichia coli BL21-Star(DE3) cells

Agarivorans albus

A8W969

752013

expressed in Pichia pastoris strain GS115

2 entries

expression in Escherichia coli

Pseudoalteromonas sp.

A1A3Y9

680864

expression in Escherichia coli with C-terminal His-tag

Pseudoalteromonas sp.

A1A3Y9

681563

the mature agarase is highly expressed extracellularly in Escherichia coli BL21 (DE3) cells

Agarivorans sp.

C9WIW7

705482