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Literature summary for 3.2.1.B1 extracted from
- Characterization and overexpression of a novel beta-agarase from Thalassomonas agarivorans (2014), J. Appl. Microbiol., 116, 563-572.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Thalassotalea agarivorans |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme remains stable in the presence of 50-200 mM NaCl (more than 80% activity) | Thalassotalea agarivorans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thalassotalea agarivorans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87613 | - |
x * 87613, calculated from amino acid sequence | Thalassotalea agarivorans |
| 90000 | - |
x * 90000, SDS-PAGE | Thalassotalea agarivorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agar + H2O | Thalassotalea agarivorans | 100% activity towards biological agar. The relative activities to agarose for electrophoresis, edible agar and low-melting agar are 75.9, 70.9 and 49.3%, respectively | neoagarobiose + neoagarotetraose + neoagarohexaose | neoagarobiose is the main product | ? |  |
| agar + H2O | Thalassotalea agarivorans BCRC 17492 | 100% activity towards biological agar. The relative activities to agarose for electrophoresis, edible agar and low-melting agar are 75.9, 70.9 and 49.3%, respectively | neoagarobiose + neoagarotetraose + neoagarohexaose | neoagarobiose is the main product | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thalassotalea agarivorans | U3GSN9 | - |
- |
| Thalassotalea agarivorans BCRC 17492 | U3GSN9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, and gel filtration | Thalassotalea agarivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agar + H2O | 100% activity towards biological agar. The relative activities to agarose for electrophoresis, edible agar and low-melting agar are 75.9, 70.9 and 49.3%, respectively | Thalassotalea agarivorans | neoagarobiose + neoagarotetraose + neoagarohexaose | neoagarobiose is the main product | ? | |
| agar + H2O | 100% activity towards biological agar. The relative activities to agarose for electrophoresis, edible agar and low-melting agar are 75.9, 70.9 and 49.3%, respectively | Thalassotalea agarivorans BCRC 17492 | neoagarobiose + neoagarotetraose + neoagarohexaose | neoagarobiose is the main product | ? | |
| additional information | does not hydrolyze lambda-carrageenan, kappa-carrageenan and, iota-carrageenan | Thalassotalea agarivorans | ? | - |
? | |
| additional information | does not hydrolyze lambda-carrageenan, kappa-carrageenan and, iota-carrageenan | Thalassotalea agarivorans BCRC 17492 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90000, SDS-PAGE | Thalassotalea agarivorans |
| ? | x * 87613, calculated from amino acid sequence | Thalassotalea agarivorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgaB1 | - |
Thalassotalea agarivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Thalassotalea agarivorans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | optimal temperature is at 40°C, while activity is dramatically dropped to 28% at 45°C and completely lost at 50°C | Thalassotalea agarivorans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | the residual activity is above 79% at 30 and 35°C. The half-life is 56 min at 40°C | Thalassotalea agarivorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Thalassotalea agarivorans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | the relative activities at pH 7.0 and 8.0 are 91.2 and 89.7%, respectively. At pH 9.0, the relative activity falls to 38.7%. No detectable activity is observed at pH 6.0 and 10.0 | Thalassotalea agarivorans |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thalassotalea agarivorans | calculated from amino acid sequence | - |
5.1 |
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