3.2.1.B1: extracellular agarase

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For detailed information about extracellular agarase, go to the full flat file.

Reaction

hydrolysis of 1,3-beta-D-galactosidic linkages in agarose, giving the octamer as the predominant product =

Synonyms

Aga21, AgaA, AgaA7, AgaB, AgaB1, AgaC, AgaG1, agaM1, agarase AG-b, agarase-a, agarase-b, beta-agarase, beta-agarase AgaB, endo-acting beta-agarase, endo-type beta-agarase, exo-beta-agarase, extracellular beta-agarase, NifU, YM01-3

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.B1 extracellular agarase

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Results	in table
11	Activating Compound
7	AA Sequence
1	Application
12	Cloned(Commentary)
3	Expression
3	General Stability
1	IC50 Value
167	Inhibitors
1	kcat/KM [mM/s]
4	KM Value [mM]
40	Localization
47	Metals/Ions
24	Molecular Weight [Da]
29	Natural Substrates/ Products (Substrates)
50	Organism
27	pH Optimum
17	pH Range
17	pH Stability
4	pI Value
1	Posttranslational Modification
5	Protein Variants
22	Purification (Commentary)
2	Reaction
48	Reference
1	Renatured (Commentary)
10	Source Tissue
15	Specific Activity [micromol/min/mg]
1	Storage Stability
88	Substrates and Products (Substrate)
47	Subunits
49	Synonyms
1	Systematic Name
29	Temperature Optimum [°C]
19	Temperature Range [°C]
27	Temperature Stability [°C]
6	Turnover Number [1/s]

Purification

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Purification on EC 3.2.1.B1 - extracellular agarase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

2 entries

ammonium sulfate precipitation and DEAE Sepharose column chromatography

Agarivorans albus

A8W969

752013

ammonium sulfate precipitation and DEAE-cellulose column chromatography

Acinetobacter junii

-

749633

ammonium sulfate precipitation and dialysis

Alteromonas sp.

-

732392

ammonium sulfate precipitation and HiTrap DEAE column chromatography

Microbulbifer sp. BN3

-

750807

ammonium sulfate precipitation method and dialysis

Alteromonas sp.

-

751409

ammonium sulfate precipitation, DEAE column chromatography, and Q-Sepharose column chromatography

Agarivorans albus

-

732848

ammonium sulfate precipitation, DEAE Sepharose column chromatography, and DEAE Sephadex A-50 gel filtration

Pseudomonas sp.

-

731420, 750000

ammonium sulfate precipitation, Q-Sepharose column chromatography and Sephacryl S-200 gel filtration

Bacillus sp. (in: Bacteria)

-

732328

ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl S-200 gel filtration

Pseudoalteromonas sp. NJ21

-

750214

ammonium sulfate precipitation, Resource Q column chromatography, and Superdex 200 gel filtration

Pseudoalteromonas hodoensis

-

731165

ammonium sulfate precipitation, Toyopearl QAE-550C column chromatography, Toyopearl HW-55F column chromatography, and MonoQ column chromatography

Cellvibrio sp.

-

715875

DEAE-Sepharose column chromatography and Sephacryl S-100HR gel filtration

Stenotrophomonas sp. NTa

-

750831

glutathione Sepharose column chromatography, and gel filtration

Alteromonas sp.

U3PAU3

731208

Ni Sepharose column chromatography

Microbulbifer sp. Q7

-

749549

Ni-NTA column chromatography

Agarivorans sp.

C9WIW7

705482

Ni-NTA column chromatography, and gel filtration

Thalassotalea agarivorans

U3GSN9

731995

partial purification by ammonium sulfate precipitation

Rhodococcus sp. Q5

-

749732

recombinant protein, purification from inclusion bodies

Pseudoalteromonas sp.

A1A3Y9

681563

ultrafiltration, DEAE-Sepharose column chromatography, and Sephacryl S-300HR gel filtration

Microbulbifer sp.

-

714561

-

Vibrio sp.

-

699362

-

Catenovulum agarivorans

T1WFD0

732434