Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Cellvibrio sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE | Cellvibrio sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellvibrio sp. | - |
- |
- |
Cellvibrio sp. KY-YJ-3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Toyopearl QAE-550C column chromatography, Toyopearl HW-55F column chromatography, and MonoQ column chromatography | Cellvibrio sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7 | - |
enzyme from culture fluid, at pH 7.0 and 35°C | Cellvibrio sp. |
84.2 | - |
after 120.2fold purification, at pH 7.0 and 35°C | Cellvibrio sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agarose + H2O | the enzyme cleaves the beta-1,4-linkage in agarose to produce neoagarooligosaccharides mainly composed of neoagarobiose, neoagarotetraose, and neoagarohexaose | Cellvibrio sp. | neoagarobiose + neoagarotetraose + neoagarohexaose | main products | ? | |
agarose + H2O | the enzyme cleaves the beta-1,4-linkage in agarose to produce neoagarooligosaccharides mainly composed of neoagarobiose, neoagarotetraose, and neoagarohexaose | Cellvibrio sp. KY-YJ-3 | neoagarobiose + neoagarotetraose + neoagarohexaose | main products | ? | |
additional information | the purified enzyme fails to hydrolyze carboxymethylcellulose, dextran, soluble starch, pectin, and polygalacturonic acid | Cellvibrio sp. | ? | - |
? | |
additional information | the purified enzyme fails to hydrolyze carboxymethylcellulose, dextran, soluble starch, pectin, and polygalacturonic acid | Cellvibrio sp. KY-YJ-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Cellvibrio sp. |
Synonyms | Comment | Organism |
---|---|---|
extracellular beta-agarase | - |
Cellvibrio sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Cellvibrio sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | more than 90% activity between 40°C and 50°C | Cellvibrio sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Cellvibrio sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | more than 90% activity between pH 5.5 and pH 7.6 | Cellvibrio sp. |