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3.2.1.80: fructan beta-fructosidase

This is an abbreviated version!
For detailed information about fructan beta-fructosidase, go to the full flat file.

Word Map on EC 3.2.1.80

Reaction

1,1,1-kestose
+
H2O
=
beta-D-fructose
 +
sucrose

Synonyms

(6&1) FEH, 1-FEH, 1-FEH I, 1-FEH II, 1-FEH IIa, 1-FEHa, 1-KEH, 1-kestose exohydrolase, 2,1-beta-D-fructan fructohydrolase, 6&1-FEH, 6&1-FEH w1, 6-FEH, 6FEH1SM, 6G&1-FEH, aoeh4a, aoeh4b, AtcwINV1 D239A mutant, AtcwINV3, AtcwINV6, beta-D-fructan fructanohydrolase, BfrA, Ci1-1-FEH IIa, D31, EC 3.2.1.153, exo-beta-D-fructosidase, exo-beta-fructosidase, exoinulinase, FEH, FEH1, fructan 1-exohydrolase, fructan 1-exohydrolase I, fructan 1-exohydrolase II, fructan 1-exohydrolase IIa, fructan 6-exohydrolase, fructan 6G&1-exohydrolase, fructan beta-fructosidase, fructan exohydrolase, Fructanase, fructofuranosidase, polysaccharide beta, fructofuranosyl hydrolase, glycoside hydrolase, Ht1-FEH III, inuD, inuE, inulinase, invertase, levanase, Lp6-FEHa, More, P-I, polysaccharide beta-fructofuranosidase, sacCp, Wfh-sm1, Wfh-sm3

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.80 fructan beta-fructosidase

Specific Activity

Specific Activity on EC 3.2.1.80 - fructan beta-fructosidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.2
-
reaction mixture consists of 0.1 ml purified inulinase, 0.9 ml acetate buffer, 20 g/l inulin; incubation at 50°C for 10 min; reaction inactivated at 100°C for 10 min
1.765
-
enzyme from supernatant, at pH 6.5 and 30°C
12.2
-
isoform 1-FEH w1
12300
-
pH 5.0, 50°C
13.65
-
native protein, pH 5.0
14.6
-
isoform 1-FEH w1
15
purified recombinant enzyme, pH 5.0, 30°C
150
-
substrate: sucrose, recombinant InuE
156
at 55ºC, pH 4.5
171
-
substrate: sucrose, InuE:D' hybrid
25.9
purified native enzyme, pH 5.0, 30°C
26.6
-
protein expressed in Pichia pastoris, pH 5.0
280
-
substrate: inulin,InuE:D' hybrid
4.02
-
crude extract, at pH 5.0 and 50°C
40.4
-
substrate: inulin, recombinant InuE
49.5
-
after 12.3fold purification, at pH 5.0 and 50°C
73.4
-
after 41.6fold purification, at pH 6.5 and 30°C
743
inulin, at 40ºC, pH 5.0
93.8
sucrose, at 40ºC, pH 5.0
additional information