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3.2.1.80: fructan beta-fructosidase

This is an abbreviated version!
For detailed information about fructan beta-fructosidase, go to the full flat file.

Word Map on EC 3.2.1.80

Reaction

1,1,1-kestose
+
H2O
=
beta-D-fructose
 +
sucrose

Synonyms

(6&1) FEH, 1-FEH, 1-FEH I, 1-FEH II, 1-FEH IIa, 1-FEHa, 1-KEH, 1-kestose exohydrolase, 2,1-beta-D-fructan fructohydrolase, 6&1-FEH, 6&1-FEH w1, 6-FEH, 6FEH1SM, 6G&1-FEH, aoeh4a, aoeh4b, AtcwINV1 D239A mutant, AtcwINV3, AtcwINV6, beta-D-fructan fructanohydrolase, BfrA, Ci1-1-FEH IIa, D31, EC 3.2.1.153, exo-beta-D-fructosidase, exo-beta-fructosidase, exoinulinase, FEH, FEH1, fructan 1-exohydrolase, fructan 1-exohydrolase I, fructan 1-exohydrolase II, fructan 1-exohydrolase IIa, fructan 6-exohydrolase, fructan 6G&1-exohydrolase, fructan beta-fructosidase, fructan exohydrolase, Fructanase, fructofuranosidase, polysaccharide beta, fructofuranosyl hydrolase, glycoside hydrolase, Ht1-FEH III, inuD, inuE, inulinase, invertase, levanase, Lp6-FEHa, More, P-I, polysaccharide beta-fructofuranosidase, sacCp, Wfh-sm1, Wfh-sm3

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.80 fructan beta-fructosidase

Inhibitors

Inhibitors on EC 3.2.1.80 - fructan beta-fructosidase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C
2,5 dideoxy-2,5-imino-D-mannitol
-
Al3+
at 55ÂșC, pH 4.5, 5 mM, 89% inhibition
Ba2+
-
more than 50% of residual enzymatic activity at 20 mM
CaCl2
-
partially
CuSO4
-
1 mM, 47% inhibition
D-glucose
Fe2(SO4)3
-
1 mM, 38% inhibition
iodoacetate
-
30 min at 30ÂșC, 10 mM, 87% inhibition; 30 min at 30ÂșC, 1 mM, 38% inhibition; 30 min at 30ÂșC, 5 mM, 57% inhibition
iodoacetic acid
-
strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C
N-bromosuccinimide
1.0 mM, pH 5.0, at 30ÂșC, 100% inhibition
p-chloromercuribenzoate
phenylmethanesulfonyl fluoride
-
strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C
SDS
-
final concentration of 10.0 mM
Sodium arsenate
-
30 min at 30ÂșC, 10 mM, 50% inhibition; 30 min at 30ÂșC, 1 mM, 20% inhibition; 30 min at 30ÂșC, 5 mM, 38% inhibition
sodium arsenite
-
30 min at 30ÂșC, 10 mM, 58% inhibition; 30 min at 30ÂșC, 1 mM, 28% inhibition; 30 min at 30ÂșC, 5 mM, 41% inhibition
sucrose
additional information
-