Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.80 extracted from

  • Moriyama, S.; Ohta, K.
    Functional characterization and evolutionary implication of the internal 157-amino-acid sequence of an exoinulinase from Penicillium sp. strain TN-88 (2007), J. Biosci. Bioeng., 103, 293-297.
    View publication on PubMed
Search for more literature for 3.2.1.80

Cloned(Commentary)

Cloned (Comment) Organism
the internal region D' encoding the 157-amino-acid sequence in the Penicillium exoinulinase gene inuD cDNA is inserted into the site between the nucleotides 897 and 898 of the Aspergillus niger exoinulinase gene inuE cDNA. The resultant inuE:D' fusion is expressed in Pichia pastoris Penicillium sp.

Protein Variants

Protein Variants Comment Organism
additional information the internal region D' encoding the 157-amino-acid sequence in the Penicillium exoinulinase gene inuD cDNA is inserted into the site between the nucleotides 897 and 898 of the Aspergillus niger exoinulinase gene inuE cDNA. The resultant inuE:D' fusion is expressed in Pichia pastoris. The Km-value of the secreted hybrid enzyme InuE:D' for inulin hydrolysis is about 1/15 that of the Aspergillus niger InuE, whereas its kcat value does not differ greatly from that of the InuE Penicillium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 inulin InuE:D' hybrid Penicillium sp.
3
-
 inulin InuE Penicillium sp.
48.7
-
 sucrose InuE:D' hybrid Penicillium sp.
111
-
 sucrose InuE Penicillium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Penicillium sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73924
-
 x * 73924, InuE:D hybrid, calculated from sequence Penicillium sp.
110000
-
 x * 110000, InuE:D hybrid, SDS-PAGE Penicillium sp.

Organism

Organism UniProt Comment Textmining
Penicillium sp.
-
-
-
Penicillium sp. TN-88
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant inuE and inuE:D' fusion protein. The internal region D' encoding the 157-amino-acid sequence in the Penicillium exoinulinase gene inuD cDNA is inserted into the site between the nucleotides 897 and 898 of the Aspergillus niger exoinulinase gene inuE cDNA. The resultant inuE:D' fusion is expressed in Pichia pastoris and purified Penicillium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.4
-
 substrate: inulin, recombinant InuE Penicillium sp.
150
-
 substrate: sucrose, recombinant InuE Penicillium sp.
171
-
 substrate: sucrose, InuE:D' hybrid Penicillium sp.
280
-
 substrate: inulin,InuE:D' hybrid Penicillium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O
-
 Penicillium sp. D-fructose + ?
-
 ?
inulin + H2O
-
 Penicillium sp. TN-88 D-fructose + ?
-
 ?
sucrose + H2O
-
 Penicillium sp. D-fructose + D-glucose
-
 ?
sucrose + H2O
-
 Penicillium sp. TN-88 D-fructose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 110000, InuE:D' hybrid, SDS-PAGE Penicillium sp.
? x * 73924, InuE:D' hybrid, calculated from sequence Penicillium sp.

Synonyms

Synonyms Comment Organism
inuE
-
 Penicillium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 substrate: inulin, InuE:D' hybrid Penicillium sp.
60
-
 substrate: inulin or sucrose, recombinant enzyme InuE Penicillium sp.
60
-
 substrate: sucrose, InuE:D' hybrid Penicillium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 30 min, inulinase activity of InuE:D' is stable below Penicillium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
500
-
 inulin InuE Penicillium sp.
1110
-
 inulin InuE:D' hybrid Penicillium sp.
3330
-
 sucrose InuE:D' hybrid Penicillium sp.
5000
-
 sucrose InuE Penicillium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 substrate: inulin or sucrose, recombinant enzyme InuE Penicillium sp.
4.5
-
 substrate: inulin and sucrose, InuE:D' hybrid Penicillium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10 30°C, 24 h, InuE:D', stable Penicillium sp.