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3.2.1.80: fructan beta-fructosidase

This is an abbreviated version!
For detailed information about fructan beta-fructosidase, go to the full flat file.

Word Map on EC 3.2.1.80

Reaction

1,1,1-kestose
+
H2O
=
beta-D-fructose
 +
sucrose

Synonyms

(6&1) FEH, 1-FEH, 1-FEH I, 1-FEH II, 1-FEH IIa, 1-FEHa, 1-KEH, 1-kestose exohydrolase, 2,1-beta-D-fructan fructohydrolase, 6&1-FEH, 6&1-FEH w1, 6-FEH, 6FEH1SM, 6G&1-FEH, aoeh4a, aoeh4b, AtcwINV1 D239A mutant, AtcwINV3, AtcwINV6, beta-D-fructan fructanohydrolase, BfrA, Ci1-1-FEH IIa, D31, EC 3.2.1.153, exo-beta-D-fructosidase, exo-beta-fructosidase, exoinulinase, FEH, FEH1, fructan 1-exohydrolase, fructan 1-exohydrolase I, fructan 1-exohydrolase II, fructan 1-exohydrolase IIa, fructan 6-exohydrolase, fructan 6G&1-exohydrolase, fructan beta-fructosidase, fructan exohydrolase, Fructanase, fructofuranosidase, polysaccharide beta, fructofuranosyl hydrolase, glycoside hydrolase, Ht1-FEH III, inuD, inuE, inulinase, invertase, levanase, Lp6-FEHa, More, P-I, polysaccharide beta-fructofuranosidase, sacCp, Wfh-sm1, Wfh-sm3

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.80 fructan beta-fructosidase

Purification

Purification on EC 3.2.1.80 - fructan beta-fructosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
4.2-fold purification, 21% yield
-
7.2-fold purification, analyzed by SDS-PAGE, 2.44-fold increase of specific inulinase activity with 22.4% yield after DEAE sepharose fast flow anion exchange chromatography
-
ammonium sulfate precipitation and Ni-NTA column chromatography
ammonium sulfate precipitation, ConA affinity-, ion exchange-chromatography, and gel filtration
-
by ammonium sulfate precipitation, DEAE-Sephacel column, Sephacryl S-200 column, concanavalin A-linked amino-activated silica column and Sepharose 6B column, 25fold
-
by ammonium sulfate precipitation, Sephadex column and DEAE-cellulose column, 62fold
-
by ultrafiltration, DEAE-cellulofine column, Q-Sepharose column and Superdex column, 329fold
centrifugation, concentration and desaltation with 20 mM citrate-phosphate buffer pH 5.0 on Amicon Ultra-15 concentrator
-
DEAE-Sepharose CL-6B column chromatography, Toyopearl HW55S column chromatography, and Toyopearl HW55S column chromatography
-
native enzyme 700fold from roots by anion exchange chromatography, gel filtration step 1, hydrophobic interaction chromatography, and 2fold gel filtration step 2. Recombinant enzyme 99fold from Pichia pastoris by anion exchange chromatography and two different steps of gel filtration
purification of recombinant inuE and inuE:D' fusion protein. The internal region D' encoding the 157-amino-acid sequence in the Penicillium exoinulinase gene inuD cDNA is inserted into the site between the nucleotides 897 and 898 of the Aspergillus niger exoinulinase gene inuE cDNA. The resultant inuE:D' fusion is expressed in Pichia pastoris and purified
-
recombinant enzyme, by ammonium sulfate precipitation and anion exchange column, 4.8fold
recombinant extracellular enzyme from Pichia pastoris strain X33 from the culture supernatant
recombinant extracellular His-tagged enzyme from Pichia pastoris strain PpBfrA(4X) culture supernatant by nickel affinity chromatography
-
recombinant His-tagged enzyme from Pichia pastoris strain X-33 by nickel affinity chromatography and ultrafiltration
-
Sephadex G-25
-
Sephadex G50 gel filtration
two independent isoforms 1-FEH IIa and 1-FEH IIb from leaves and roots
-
two isoforms 1-FEH w1 and w2
-
ultrafiltration and Mono Q Sepharose column chromatography
-
ultrafiltration, 1.3fold
-