3.2.1.80: fructan beta-fructosidase
This is an abbreviated version!
For detailed information about fructan beta-fructosidase, go to the full flat file.
Word Map on EC 3.2.1.80
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3.2.1.80
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hydrolases
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oligosaccharide
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xylans
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subsite
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carbohydrate-binding
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pastoris
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cellulases
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xylanases
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cellulolytic
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fructans
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transglycosylation
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arabinoxylans
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lignocellulose
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carbohydrate-active
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chitinases
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endoglucanases
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xylooligosaccharides
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xyloglucan
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saccharification
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endoxylanase
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avicel
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thermocellum
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hemicellulases
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xylotriose
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cellulosome
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arabinan
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beechwood
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cellobiohydrolases
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laminarin
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cazymes
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lichenan
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exoglucanase
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chitosanase
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xylanolytic
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caldicellulosiruptor
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glycone
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agarase
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dockerins
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spelt
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endo-acting
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cellotetraose
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arabinofuranosidase
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levan
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mannanase
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endo-type
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cellotriose
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cellooligosaccharides
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alpha-l-arabinofuranosidase
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fructosyltransferase
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jecorina
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synthesis
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nutrition
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diagnostics
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food industry
- 3.2.1.80
- hydrolases
- oligosaccharide
- xylans
-
subsite
-
carbohydrate-binding
- pastoris
- cellulases
- xylanases
-
cellulolytic
- fructans
-
transglycosylation
- arabinoxylans
- lignocellulose
-
carbohydrate-active
- chitinases
- endoglucanases
- xylooligosaccharides
- xyloglucan
-
saccharification
- endoxylanase
- avicel
- thermocellum
-
hemicellulases
- xylotriose
- cellulosome
- arabinan
-
beechwood
- cellobiohydrolases
- laminarin
-
cazymes
- lichenan
- exoglucanase
- chitosanase
-
xylanolytic
- caldicellulosiruptor
-
glycone
- agarase
-
dockerins
- spelt
-
endo-acting
- cellotetraose
- arabinofuranosidase
- levan
- mannanase
-
endo-type
- cellotriose
- cellooligosaccharides
- alpha-l-arabinofuranosidase
- fructosyltransferase
- jecorina
- synthesis
- nutrition
- diagnostics
- food industry
Reaction
Synonyms
(6&1) FEH, 1-FEH, 1-FEH I, 1-FEH II, 1-FEH IIa, 1-FEHa, 1-KEH, 1-kestose exohydrolase, 2,1-beta-D-fructan fructohydrolase, 6&1-FEH, 6&1-FEH w1, 6-FEH, 6FEH1SM, 6G&1-FEH, aoeh4a, aoeh4b, AtcwINV1 D239A mutant, AtcwINV3, AtcwINV6, beta-D-fructan fructanohydrolase, BfrA, Ci1-1-FEH IIa, D31, EC 3.2.1.153, exo-beta-D-fructosidase, exo-beta-fructosidase, exoinulinase, FEH, FEH1, fructan 1-exohydrolase, fructan 1-exohydrolase I, fructan 1-exohydrolase II, fructan 1-exohydrolase IIa, fructan 6-exohydrolase, fructan 6G&1-exohydrolase, fructan beta-fructosidase, fructan exohydrolase, Fructanase, fructofuranosidase, polysaccharide beta, fructofuranosyl hydrolase, glycoside hydrolase, Ht1-FEH III, inuD, inuE, inulinase, invertase, levanase, Lp6-FEHa, More, P-I, polysaccharide beta-fructofuranosidase, sacCp, Wfh-sm1, Wfh-sm3
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Reference on EC 3.2.1.80 - fructan beta-fructosidase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Norman, J.M.; Bunny, K.L.; Giffard, P.M.
Characterization of levJ, a sucrase/fructanase-encoding gene from Actinomyces naeslundii T14V, and comparison of its product with other sucrose-cleaving enzymes
Gene
152
93-98
1995
Actinomyces naeslundii, Actinomyces naeslundii T14V
Burne, R.A.; Schilling, K.; Bowen, W.H.; Yasbin, R.E.
Expression, purification, and characterization of an exo-beta-D-fructosidase of Streptococcus mutans
J. Bacteriol.
169
4507-4517
1987
Streptococcus mutans, Streptococcus mutans GS-5
Takahashi, N.; Mizuno, F.; Takamori, K.
Purification and preliminary characterization of exo-beta-D-fructosidase in Streptococcus salivarius KTA-19
Infect. Immun.
47
271-276
1985
Streptococcus salivarius
Frehner, M.; Keller, F.; Wiemken, A.
Localisation of fructan metabolism in the vacuoles isolated from protoplasts of jerusalem artichoke tubers (Helianthus tuberosus L.)
J. Plant Physiol.
116
197-208
1984
Helianthus tuberosus
Igarashi, T.; Yakamoto, A.; Goto, N.
Characterization of an exo-beta-D-fructosidase from Streptococcus mutants ingbritt
Microbiol. Immunol.
36
643-647
1992
Streptococcus mutans
Burne, R.A.; Penders, J.E.C.
Characterization of the Streptococcus mutans GS-5 fruA gene encoding exo-beta-D-fructosidase
Infect. Immun.
60
4621-4632
1992
Streptococcus mutans, Streptococcus mutans GS-5
Henson, C.A.; Livingston III, D.P.
Purification and characterization of an oat fructan exohydrolase that preferentially hydrolyzes beta-2,6-fructans
Plant Physiol.
110
639-644
1996
Avena sativa
Henson, C.A.; Livingston III, D.P.
Characterization of a fructan exohydrolase purified from barley stems that hydrolyzes multiple fructofuranosidic linkages
Plant Physiol. Biochem.
36
715-720
1998
Hordeum vulgare
-
De Roover, J.; Van Laere, A.; De Winter, M.; Timmermanns, J.W.; Van den Ende, W.
Purification and properties of a second fructan exohydrolase from the roots of Chicorium intybus
Physiol. Plant.
106
28-34
1999
Cichorium intybus
-
Verhaest, M.; Van den Ende, W.; Yoshida, M.; Le Roy, K.; Peeraer, Y.; Sansen, S.; De Ranter, C.J.; Van Laere, A.; Rabijns, A.
Crystallization and preliminary X-ray diffraction study of fructan 1-exohydrolase IIa from Cichorium intybus
Acta Crystallogr. Sect. D
60
553-554
2004
Cichorium intybus
Gill, P.K.; Manhas, R.K.; Singh, J.; Singh, P.
Purification and characterization of an exoinulinase from Aspergillus fumigatus
Appl. Biochem. Biotechnol.
117
19-32
2004
Aspergillus fumigatus
Kulminskaya, A.A.; Arand, M.; Eneyskaya, E.V.; Ivanen, D.R.; Shabalin, K.A.; Shishlyannikov, S.M.; Saveliev, A.N.; Korneeva, O.S.; Neustroev, K.N.
Biochemical characterization of Aspergillus awamori exoinulinase: substrate binding characteristics and regioselectivity of hydrolysis
Biochim. Biophys. Acta
1650
22-29
2003
Aspergillus awamori (Q96TU3), Aspergillus awamori
Moriyama, S.; Akimoto, H.; Suetsugu, N.; Kawasaki, S.; Nakamura, T.; Ohta, K.
Purification and properties of an extracellular exoinulinase from Penicillium sp. strain TN-88 and sequence analysis of the encoding gene
Biosci. Biotechnol. Biochem.
66
1887-1896
2002
Penicillium sp. (Q8J0G1), Penicillium sp., Penicillium sp. TN-88 (Q8J0G1)
Jing, W.; Zhengyu, J.; Bo, J.; Xueming, X.
Separation and identification of exo- and endoinulinase from Aspergillus ficuum
Curr. Microbiol.
47
109-112
2003
Aspergillus ficuum, Aspergillus ficuum JNSP5-06
Hamdy, H.S.
Purification and some important characters of extracellular inulinase of Alternaria alternata (Fr.) Keissler
Indian J. Exp. Biol.
40
1393-1398
2002
Alternaria alternata
Michiels, A.; Van Laere, A.; Van den Ende, W.; Tucker, M.
Expression analysis of a chicory fructan 1-exohydrolase gene reveals complex regulation by cold
J. Exp. Bot.
55
1325-1333
2004
Cichorium intybus (Q93X60), Cichorium intybus
Van den Ende, W.; Michiels, A.; De Roover, J.; Verhaert, P.; Van Laere, A.
Cloning and functional analysis of chicory root fructan1-exohydrolase I (1-FEH I): a vacuolar enzyme derived from a cell-wall invertase ancestor? Mass fingerprint of the 1-FEH I enzyme
Plant J.
24
447-456
2000
Cichorium intybus
Van den Ende, W.; De Coninck, B.; Clerens, S.; Vergauwen, R.; Van Laere, A.
Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel 'cell-wall invertase-like' specific 6-FEH from sugar beet (Beta vulgaris L.)
Plant J.
36
697-710
2003
Beta vulgaris
Van den Ende, W.; Michiels, A.; Van Wonterghem, D.; Clerens, S.P.; De Roover, J.; Van Laere, A.J.
Defoliation induces fructan 1-exohydrolase II in witloof chicory roots. Cloning and purification of two isoforms, fructan 1-exohydrolase IIa and fructan 1-exohydrolase IIb. Mass fingerprint of the fructan 1-exohydrolase II enzymes
Plant Physiol.
126
1186-1195
2001
Cichorium intybus
Van Den Ende, W.; Clerens, S.; Vergauwen, R.; Van Riet, L.; Van Laere, A.; Yoshida, M.; Kawakami, A.
Fructan 1-exohydrolases. beta-(2,1)-trimmers during graminan biosynthesis in stems of wheat? Purification, characterization, mass mapping, and cloning of two fructan 1-exohydrolase isoforms
Plant Physiol.
131
621-631
2003
Triticum aestivum
Zhang, L.; Zhao, C.; Zhu, D.; Ohta, Y.; Wang, Y.
Purification and characterization of inulinase from Aspergillus niger AF10 expressed in Pichia pastoris
Protein Expr. Purif.
35
272-275
2004
Aspergillus niger (O74641), Aspergillus niger AF10 (O74641), Aspergillus niger AF10
Paludan-Muller, C.; Gram, L.; Rattray, F.P.
Purification and characterisation of an extracellular fructan beta-fructosidase from a Lactobacillus pentosus strain isolated from fermented fish
Syst. Appl. Microbiol.
25
13-20
2002
Lactiplantibacillus pentosus, Lactiplantibacillus pentosus B235
Uzunova, K.; Vassileva, A.; Kambourova, M.; Ivanova, V.; Spasova, D.; Mandeva, R.; Derekova, A.; Tonkova, A.
Production and properties of a bacterial thermostable exo-inulinase
Z. Naturforsch. C
56
1022-1028
2001
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) 11
Verhaest, M.; Ende, W.V.; Roy, K.L.; De Ranter, C.J.; Laere, A.V.; Rabijns, A.
X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus
Plant J.
41
400-411
2005
Cichorium intybus (Q93X60), Cichorium intybus
Yang, J.; Zhang, J.; Wang, Z.; Zhu, Q.; Liu, L.
Activities of fructan- and sucrose-metabolizing enzymes in wheat stems subjected to water stress during grain filling
Planta
220
331-343
2004
Triticum aestivum
Benkeblia, N.; Ueno, K.; Onodera, S.; Shiomi, N.
Variation of fructooligosaccharides and their metabolizing enzymes in onion bulb (Allium cepa L. cv. Tenshin) during long-term storage
J. Food Sci.
70
S208-S214
2005
Allium cepa
Kawakami, A.; Yoshida, M.; Van den Ende, W.
Molecular cloning and functional analysis of a novel 6&1-FEH from wheat (Triticum aestivum L.) preferentially degrading small graminans like bifurcose
Gene
358
93-101
2005
Triticum aestivum
Van Riet, L.; Nagaraj, V.; Van den Ende, W.; Clerens, S.; Wiemken, A.; van Laere, A.
Purification, cloning and functional characterization of a fructan 6-exohydrolase from wheat (Triticum aestivum L.)
J. Exp. Bot.
57
213-223
2006
Triticum aestivum
Nagem, R.A.; Rojas, A.L.; Golubev, A.M.; Korneeva, O.S.; Eneyskaya, E.V.; Kulminskaya, A.A.; Neustroev, K.N.; Polikarpov, I.
Crystal structure of exo-inulinase from Aspergillus awamori: the enzyme fold and structural determinants of substrate recognition
J. Mol. Biol.
344
471-480
2004
Aspergillus awamori, Aspergillus awamori EI
De Coninck, B.; Le Roy, K.; Francis, I.; Clerens, S.; Vergauwen, R.; Halliday, A.M.; Smith, S.M.; Van Laere, A.; van den Ende, W.
Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities
Plant Cell Environ.
28
432-443
2005
Arabidopsis thaliana
-
Portes, M.T.; Carvalho, M.A.
Spatial distribution of fructans and fructan metabolizing enzymes in rhizophores of Vernonia herbacea (Vell.) Rusby (Asteraceae) in different developmental phases
Plant Sci.
170
624-633
2006
Vernonia herbacea, Vernonia herbacea (A9JIF3)
Van den Ende, W.; De Coninck, B.; Van Laere, A.
Plant fructan exohydrolases: a role in signaling and defense?
Trends Plant Sci.
9
523-528
2004
Arabidopsis thaliana, Beta vulgaris, Cichorium intybus
Singh, R.S.; Sooch, B.S.; Puri, M.
Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1
Biores. Technol.
98
2518-2525
2007
Kluyveromyces marxianus, Kluyveromyces marxianus Ys-1
Singh, R.S.; Bhermi, H.K.
Production of extracellular exoinulinase from Kluyveromyces marxianus YS-1 using root tubers of Asparagus officinalis
Biores. Technol.
99
7418-7423
2008
Kluyveromyces marxianus, Kluyveromyces marxianus Ys-1
Zhang, W.; Ge, X.
Improvement of fructanohydrolase production in Aspergillus niger SL-09 by sucrose ester
Food Technol. Biotechnol.
44
59-64
2006
Aspergillus niger, Aspergillus niger SL-09
-
Santos, A.M.; Maugeri, F.
Synthesis of fructooligosaccharides from sucrose using inulinase from Kluyveromyces marxianus
Food Technol. Biotechnol.
45
181-186
2007
Kluyveromyces marxianus
-
Moriyama, S.; Muguruma, M.; Ohta, K.
Quantitative expression analysis of inulinase gene cluster of Penicillium sp. strain TN-88
J. Biosci. Bioeng.
101
277-279
2006
Penicillium sp. (Q8J0G1), Penicillium sp., Penicillium sp. TN-88 (Q8J0G1)
Moriyama, S.; Ohta, K.
Functional characterization and evolutionary implication of the internal 157-amino-acid sequence of an exoinulinase from Penicillium sp. strain TN-88
J. Biosci. Bioeng.
103
293-297
2007
Penicillium sp., Penicillium sp. TN-88
Lothier, J.; Lasseur, B.; Le Roy, K.; Van Laere, A.; Prudhomme, M.P.; Barre, P.; Van den Ende, W.; Morvan-Bertrand, A.
Cloning, gene mapping, and functional analysis of a fructan 1-exohydrolase (1-FEH) from Lolium perenne implicated in fructan synthesis rather than in fructan mobilization
J. Exp. Bot.
58
1969-1983
2007
Lolium perenne (Q64GB3), Lolium perenne
Narai-Kanayama, A.; Tokita, N.; Aso, K.
Dependence of fructooligosaccharide content on activity of fructooligosaccharide-metabolizing enzymes in yacon (Smallanthus sonchifolius) tuberous roots during storage
J. Food Sci.
72
S381-S387
2007
Smallanthus sonchifolius
Gong, F.; Sheng, J.; Chi, Z.; Li, J.
Inulinase production by a marine yeast Pichia guilliermondii and inulin hydrolysis by the crude inulinase
J. Ind. Microbiol. Biotechnol.
34
179-185
2007
Meyerozyma guilliermondii
Singh, R.S.; Dhaliwal, R.; Puri, M.
Partial purification and characterization of exoinulinase from Kluyveromyces marxianus YS-1 for preparation of high-fructose syrup
J. Microbiol. Biotechnol.
17
733-738
2007
Kluyveromyces marxianus, Kluyveromyces marxianus Ys-1
Gao, L.; Chi, Z.; Sheng, J.; Wang, L.; Li, J.; Gong, F.
Inulinase-producing marine yeasts: evaluation of their diversity and inulin hydrolysis by their crude enzymes
Microb. Ecol.
54
722-729
2007
Debaryomyces hansenii, Meyerozyma guilliermondii, Papiliotrema aurea, Debaryomyces hansenii G7a1, Papiliotrema aurea G7a, Meyerozyma guilliermondii OUC1
Pessoni, R.A.; Braga, M.R.; Figueiredo-Ribeiro, R.d.e.C.
Purification and properties of exo-inulinases from Penicillium janczewskii growing on distinct carbon sources
Mycologia
99
493-503
2007
Penicillium janczewskii
Verhaest, M.; Lammens, W.; Le Roy, K.; De Ranter, C.J.; Van Laere, A.; Rabijns, A.; Van den Ende, W.
Insights into the fine architecture of the active site of chicory fructan 1-exohydrolase: 1-kestose as substrate vs sucrose as inhibitor
New Phytol.
174
90-100
2007
Cichorium intybus (Q93X60), Cichorium intybus
Le Roy, K.; Verhaest, M.; Rabijns, A.; Clerens, S.; Van Laere, A.; Van den Ende, W.
N-glycosylation affects substrate specificity of chicory fructan 1-exohydrolase: evidence for the presence of an inulin binding cleft
New Phytol.
176
317-324
2007
Cichorium intybus, Cichorium intybus (Q93X60)
Le Roy, K.; Lammens, W.; Van Laere, A.; Van den Ende, W.
Influencing the binding configuration of sucrose in the active sites of chicory fructan 1-exohydrolase and sugar beet fructan 6-exohydrolase
New Phytol.
178
572-580
2008
Cichorium intybus (Q93X60), Cichorium intybus
Le Roy, K.; Lammens, W.; Verhaest, M.; De Coninck, B.; Rabijns, A.; Van Laere, A.; Van den Ende, W.
Unraveling the difference between invertases and fructan exohydrolases: a single amino acid (Asp-239) substitution transforms Arabidopsis cell wall invertase1 into a fructan 1-exohydrolase
Plant Physiol.
145
616-625
2007
Arabidopsis thaliana (Q67XZ3)
Sheng, J.; Chi, Z.; Gong, F.; Li, J.
Purification and Characterization of Extracellular Inulinase from a Marine Yeast Cryptococcus aureus G7a and Inulin Hydrolysis by the Purified Inulinase
Appl. Biochem. Biotechnol.
144
111-121
2008
Papiliotrema aurea, Papiliotrema aurea G7a
Mutanda, T.; Wilhelmi, B.; Whiteley, C.G.
Controlled production of fructose by an exoinulinase from Aspergillus ficuum
Appl. Biochem. Biotechnol.
159
65-77
2009
Aspergillus ficuum
Zhang, J.; Dell, B.; Conocono, E.; Waters, I.; Setter, T.; Appels, R.
Water deficits in wheat: Fructan exohydrolase (1-FEH) mRNA expression and relationship to soluble carbohydrate concentrations in two varieties
New Phytol.
181
843-850
2009
Triticum aestivum
Martel, C.M.; Parker, J.E.; Jackson, C.J.; Warrilow, A.G.; Rolley, N.; Greig, C.; Morris, S.M.; Donnison, I.S.; Kelly, D.E.; Kelly, S.L.
Expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanol
Biores. Technol.
102
1503-1508
2011
Bacillus subtilis (P05656), Bacillus subtilis
Arrizon, J.; Morel, S.; Gschaedler, A.; Monsan, P.
Purification and substrate specificities of a fructanase from Kluyveromyces marxianus isolated from the fermentation process of Mezcal
Biores. Technol.
102
3298-3303
2011
Kluyveromyces marxianus
Ueno, K.; Ishiguro, Y.; Yoshida, M.; Onodera, S.; Shiomi, N.
Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium lappa L.)
Chem. Cent. J.
5
16
2011
Arctium lappa
Tamura, K.I.; Sanada, Y.; Tase, K.; Komatsu, T.; Yoshida, M.
Pp6-FEH1 encodes an enzyme for degradation of highly polymerized levan and is transcriptionally induced by defoliation in timothy (Phleum pratense L.)
J. Exp. Bot.
62
3421-3431
2011
Phleum pratense
Kawakami, A.; Yoshida, M.
Graminan breakdown by fructan exohydrolase induced in winter wheat inoculated with snow mold
J. Plant Physiol.
169
294-302
2012
Triticum aestivum
Martinez, D.; Cutino-Avila, B.; Perez, E.R.; Menendez, C.; Hernandez, L.; Del Monte-Martinez, A.
A thermostable exo-beta-fructosidase immobilised through rational design
Food Chem.
145
826-831
2014
Thermotoga maritima
Lothier, J.; Van Laere, A.; Prudhomme, M.P.; Van den Ende, W.; Morvan-Bertrand, A.
Cloning and characterization of a novel fructan 6-exohydrolase strongly inhibited by sucrose in Lolium perenne
Planta
240
629-643
2014
Lolium perenne (D0PQE8), Lolium perenne, Lolium perenne Bravo (D0PQE8)
Shi, Q.; Hou, Y.; Xu, Y.; Moerkeberg Krogh, K.B.R.; Tenkanen, M.
Enzymatic analysis of levan produced by lactic acid bacteria in fermented doughs
Carbohydr. Polym.
208
285-293
2019
Paenibacillus amylolyticus
Gasperl, A.; Morvan-Bertrand, A.; Prudhomme, M.P.; van der Graaff, E.; Roitsch, T.
A simple and fast kinetic assay for the determination of fructan exohydrolase activity in perennial ryegrass (Lolium perenne L.)
Front. Plant Sci.
6
1154
2015
Lolium perenne
Xu, H.; Liang, M.; Xu, L.; Li, H.; Zhang, X.; Kang, J.; Zhao, Q.; Zhao, H.
Cloning and functional characterization of two abiotic stress-responsive Jerusalem artichoke (Helianthus tuberosus) fructan 1-exohydrolases (1-FEHs)
Plant Mol. Biol.
87
81-98
2015
Helianthus tuberosus (A0A0D5A4E4), Helianthus tuberosus (A0A0D5A4L3), Helianthus tuberosus
Zhan, W.; Jin, L.; Jiao, J.; Zhang, X.; Zhang, Y.; Zhao, H.; Liang, M.
Expression and purification of plant fructan exohydrolases and their potential applications in fructose production
Int. J. Biol. Macromol.
108
9-17
2018
Helianthus tuberosus
Ueno, K.; Sonoda, T.; Yoshida, M.; Shiomi, N.; Onodera, S.
Purification, characterization, and functional analysis of a novel 6G&1-FEH mainly hydrolyzing neokestose from Asparagus
J. Exp. Bot.
69
4295-4308
2018
Asparagus officinalis (A0A2Z6G7P5), Asparagus officinalis (A0A2Z6G7T9)
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