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Literature summary for 3.2.1.80 extracted from
- Purification and Characterization of Extracellular Inulinase from a Marine Yeast Cryptococcus aureus G7a and Inulin Hydrolysis by the Purified Inulinase (2008), Appl. Biochem. Biotechnol., 144, 111-121.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | production of fuel ethanol and ultra-high fructose syrup | Papiliotrema aurea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C | Papiliotrema aurea |  |
| Ag+ | decreased activity at concentrations of 1.0 mM | Papiliotrema aurea | |
| EDTA | strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C | Papiliotrema aurea | |
| Hg2+ | decreased activity at concentrations of 1.0 mM | Papiliotrema aurea | |
| inulin | - |
Papiliotrema aurea | |
| iodoacetic acid | strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C | Papiliotrema aurea | |
| Mg2+ | decreased activity at concentrations of 1.0 mM | Papiliotrema aurea | |
| phenylmethanesulfonyl fluoride | strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C | Papiliotrema aurea | |
| SDS | final concentration of 10.0 mM | Papiliotrema aurea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 20.06 mg/ml for inulin | Papiliotrema aurea |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | fermented broth | Papiliotrema aurea | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activating at concentrations of 1.0 mM | Papiliotrema aurea | |
| Cu2+ | activating at concentrations of 1.0 mM | Papiliotrema aurea | |
| Fe2+ | activating at concentrations of 1.0 mM | Papiliotrema aurea | |
| K+ | activating at concentrations of 1.0 mM | Papiliotrema aurea | |
| Na+ | activating at concentrations of 1.0 mM | Papiliotrema aurea | |
| Zn2+ | activating | Papiliotrema aurea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
after purification, analyzed by SDS-PAGE | Papiliotrema aurea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Papiliotrema aurea | - |
marine yeast, isolated from sediment at China South See | - |
| Papiliotrema aurea G7a | - |
marine yeast, isolated from sediment at China South See | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 7.2-fold purification, analyzed by SDS-PAGE, 2.44-fold increase of specific inulinase activity with 22.4% yield after DEAE sepharose fast flow anion exchange chromatography | Papiliotrema aurea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.2 | - |
reaction mixture consists of 0.1 ml purified inulinase, 0.9 ml acetate buffer, 20 g/l inulin; incubation at 50°C for 10 min; reaction inactivated at 100°C for 10 min | Papiliotrema aurea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 65°C, 2 h, 86% residual activity after treatment at 65°C, pH below 4.0 and above 6.5 results in rapid decrease in enzyme activity | Papiliotrema aurea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | - |
Papiliotrema aurea | D-fructose + D-glucose + fructooligosaccharides | - |
? | |
| inulin + H2O | - |
Papiliotrema aurea G7a | D-fructose + D-glucose + fructooligosaccharides | - |
? | |
| inulin + H2O | - |
Papiliotrema aurea | D-fructose + fructooligosaccharides | crude inulinase | ? | |
| inulin + H2O | - |
Papiliotrema aurea G7a | D-fructose + fructooligosaccharides | crude inulinase | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exoinulinase | - |
Papiliotrema aurea |
| inulinase | - |
Papiliotrema aurea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Papiliotrema aurea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 70 | - |
Papiliotrema aurea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
above rapidly inactivating, after treatment at 65°C for 2 h still 86.0% residual activity, after treatment of 70°C for 2 h still 60.0% residual activity | Papiliotrema aurea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Papiliotrema aurea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6.5 | above and below rapid decrease of enzyme activity | Papiliotrema aurea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6.5 | rapid decrease of residual activity above and below, after treatment at pH 6.0 and 4°C for 2 h still 96.0% residual activity | Papiliotrema aurea |
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Release 2023.1 (January 2023)
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