3.2.1.80: fructan beta-fructosidase

This is an abbreviated version!
For detailed information about fructan beta-fructosidase, go to the full flat file.

Reaction

Synonyms

(6&1) FEH, 1-FEH, 1-FEH I, 1-FEH II, 1-FEH IIa, 1-FEHa, 1-KEH, 1-kestose exohydrolase, 2,1-beta-D-fructan fructohydrolase, 6&1-FEH, 6&1-FEH w1, 6-FEH, 6FEH1SM, 6G&1-FEH, aoeh4a, aoeh4b, AtcwINV1 D239A mutant, AtcwINV3, AtcwINV6, beta-D-fructan fructanohydrolase, BfrA, Ci1-1-FEH IIa, D31, EC 3.2.1.153, exo-beta-D-fructosidase, exo-beta-fructosidase, exoinulinase, FEH, FEH1, fructan 1-exohydrolase, fructan 1-exohydrolase I, fructan 1-exohydrolase II, fructan 1-exohydrolase IIa, fructan 6-exohydrolase, fructan 6G&1-exohydrolase, fructan beta-fructosidase, fructan exohydrolase, Fructanase, fructofuranosidase, polysaccharide beta, fructofuranosyl hydrolase, glycoside hydrolase, Ht1-FEH III, inuD, inuE, inulinase, invertase, levanase, Lp6-FEHa, More, P-I, polysaccharide beta-fructofuranosidase, sacCp, Wfh-sm1, Wfh-sm3

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.80 fructan beta-fructosidase

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Results	in table
5	Activating Compound
542	AA Sequence
18	Application
1	CAS Registry Number
33	Cloned(Commentary)
6	Crystallization (Commentary)
6	Expression
17	General Information
1	General Stability
87	Inhibitors
5	kcat/KM [mM/s]
1	Ki Value [mM]
34	KM Value [mM]
21	Localization
25	Metals/Ions
35	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
109	Organism
2	Pathway
10	PDB ID
36	pH Optimum
12	pH Range
13	pH Stability
14	pI Value
21	Posttranslational Modification
12	Protein Variants
38	Purification (Commentary)
2	Reaction
4	Reaction Type
91	Reference
43	Source Tissue
27	Specific Activity [micromol/min/mg]
7	Storage Stability
254	Substrates and Products (Substrate)
27	Subunits
130	Synonyms
1	Systematic Name
29	Temperature Optimum [°C]
12	Temperature Range [°C]
29	Temperature Stability [°C]
10	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 3.2.1.80 - fructan beta-fructosidase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

10°C, 10 weeks, triggered by decrease in sucrose content

Allium cepa

-

665885

20°C, 10 weeks, triggered by decrease in sucrose content

Allium cepa

-

665885

4°C, 0.02 mg/ml, 20 mM phosphate buffer, pH 7.0, stable at least for several days, 90% loss of activity after 2 weeks

Streptococcus salivarius

-

208818

50°C, pH 5.4, stable for 60 min without decrease in activity, after 12 h only 96% residual activity, after 72 h only 63.8% residual activity

Aspergillus ficuum

-

690509

65°C, 2 h, 86% residual activity after treatment at 65°C, pH below 4.0 and above 6.5 results in rapid decrease in enzyme activity

Papiliotrema aurea

-

690488

at either -15ºC or at 4ºC, 200 mM acetate buffer, pH 4.6, 2 weeks, 50% loss of activity

Alternaria alternata

-

655714

higher stability at -20°C than at 4°C

Streptococcus salivarius

-

208818