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3.2.1.80: fructan beta-fructosidase

This is an abbreviated version!
For detailed information about fructan beta-fructosidase, go to the full flat file.

Word Map on EC 3.2.1.80

Reaction

1,1,1-kestose
+
H2O
=
beta-D-fructose
 +
sucrose

Synonyms

(6&1) FEH, 1-FEH, 1-FEH I, 1-FEH II, 1-FEH IIa, 1-FEHa, 1-KEH, 1-kestose exohydrolase, 2,1-beta-D-fructan fructohydrolase, 6&1-FEH, 6&1-FEH w1, 6-FEH, 6FEH1SM, 6G&1-FEH, aoeh4a, aoeh4b, AtcwINV1 D239A mutant, AtcwINV3, AtcwINV6, beta-D-fructan fructanohydrolase, BfrA, Ci1-1-FEH IIa, D31, EC 3.2.1.153, exo-beta-D-fructosidase, exo-beta-fructosidase, exoinulinase, FEH, FEH1, fructan 1-exohydrolase, fructan 1-exohydrolase I, fructan 1-exohydrolase II, fructan 1-exohydrolase IIa, fructan 6-exohydrolase, fructan 6G&1-exohydrolase, fructan beta-fructosidase, fructan exohydrolase, Fructanase, fructofuranosidase, polysaccharide beta, fructofuranosyl hydrolase, glycoside hydrolase, Ht1-FEH III, inuD, inuE, inulinase, invertase, levanase, Lp6-FEHa, More, P-I, polysaccharide beta-fructofuranosidase, sacCp, Wfh-sm1, Wfh-sm3

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.80 fructan beta-fructosidase

Cloned

Cloned on EC 3.2.1.80 - fructan beta-fructosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a modified Bacillus subtilis levanase gene (sacC) in which the native bacterial signal sequence is replaced with a yeast alpha-factor domain is expressed in Saccharomyces cerevisiae strain AH22
enzyme expression analysis of isozymes under different water conditions, overview
-
expressed in Escherichia coli Top10 cells
expressed in Pichia pastoris
expressed in Pichia pastoris strain X33
expressed in Pichia pastoris X-33 cells
expression in Escherichia coli
expression in Pichia pastoris
expression in potato tubers
-
gene aoeh4a, DNA and amino acid sequence determination and analysis
gene aoeh4b, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Pichia pastoris
gene D31, real-time PCR quantitative expression analysis
gene FEH-III, DNA and amino acid sequence determination and analysis, FEH isozyme sequences comparisons, phylogenetic analysis, functional recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33
-
gene Lp6-FEHa, DNA and amino acid sequence determination and analysis, phylogenetic analysis, real-time quantitative PCR expression analysis, cloning in Escherichia coli, recombinant expression in Pichia pastoris strain X33, secretion of the protein to the culture medium
gene wfh-sm, real-time PCR quantitative expression analysis
heterologous expression in Pichia pastoris
heterologous expression in Pichia pastoris X-33
-
in Escherichia coli
recombinant expression of the C-terminally His-tagged extracellular enzyme in Pichia pastoris strain PpBfrA(4X), secretion to the culture medium, fed-batch fermentation
-
the enzyme gene promoter is highly expressed in etiolated leaves and cold-stored roots
the internal region D' encoding the 157-amino-acid sequence in the Penicillium exoinulinase gene inuD cDNA is inserted into the site between the nucleotides 897 and 898 of the Aspergillus niger exoinulinase gene inuE cDNA. The resultant inuE:D' fusion is expressed in Pichia pastoris
-