3.2.1.80: fructan beta-fructosidase
This is an abbreviated version!
For detailed information about fructan beta-fructosidase, go to the full flat file.
Word Map on EC 3.2.1.80
-
3.2.1.80
-
hydrolases
-
oligosaccharide
-
xylans
-
subsite
-
carbohydrate-binding
-
pastoris
-
cellulases
-
xylanases
-
cellulolytic
-
fructans
-
transglycosylation
-
arabinoxylans
-
lignocellulose
-
carbohydrate-active
-
chitinases
-
endoglucanases
-
xylooligosaccharides
-
xyloglucan
-
saccharification
-
endoxylanase
-
avicel
-
thermocellum
-
hemicellulases
-
xylotriose
-
cellulosome
-
arabinan
-
beechwood
-
cellobiohydrolases
-
laminarin
-
cazymes
-
lichenan
-
exoglucanase
-
chitosanase
-
xylanolytic
-
caldicellulosiruptor
-
glycone
-
agarase
-
dockerins
-
spelt
-
endo-acting
-
cellotetraose
-
arabinofuranosidase
-
levan
-
mannanase
-
endo-type
-
cellotriose
-
cellooligosaccharides
-
alpha-l-arabinofuranosidase
-
fructosyltransferase
-
jecorina
-
synthesis
-
nutrition
-
diagnostics
-
food industry
- 3.2.1.80
- hydrolases
- oligosaccharide
- xylans
-
subsite
-
carbohydrate-binding
- pastoris
- cellulases
- xylanases
-
cellulolytic
- fructans
-
transglycosylation
- arabinoxylans
- lignocellulose
-
carbohydrate-active
- chitinases
- endoglucanases
- xylooligosaccharides
- xyloglucan
-
saccharification
- endoxylanase
- avicel
- thermocellum
-
hemicellulases
- xylotriose
- cellulosome
- arabinan
-
beechwood
- cellobiohydrolases
- laminarin
-
cazymes
- lichenan
- exoglucanase
- chitosanase
-
xylanolytic
- caldicellulosiruptor
-
glycone
- agarase
-
dockerins
- spelt
-
endo-acting
- cellotetraose
- arabinofuranosidase
- levan
- mannanase
-
endo-type
- cellotriose
- cellooligosaccharides
- alpha-l-arabinofuranosidase
- fructosyltransferase
- jecorina
- synthesis
- nutrition
- diagnostics
- food industry
Reaction
Synonyms
(6&1) FEH, 1-FEH, 1-FEH I, 1-FEH II, 1-FEH IIa, 1-FEHa, 1-KEH, 1-kestose exohydrolase, 2,1-beta-D-fructan fructohydrolase, 6&1-FEH, 6&1-FEH w1, 6-FEH, 6FEH1SM, 6G&1-FEH, aoeh4a, aoeh4b, AtcwINV1 D239A mutant, AtcwINV3, AtcwINV6, beta-D-fructan fructanohydrolase, BfrA, Ci1-1-FEH IIa, D31, EC 3.2.1.153, exo-beta-D-fructosidase, exo-beta-fructosidase, exoinulinase, FEH, FEH1, fructan 1-exohydrolase, fructan 1-exohydrolase I, fructan 1-exohydrolase II, fructan 1-exohydrolase IIa, fructan 6-exohydrolase, fructan 6G&1-exohydrolase, fructan beta-fructosidase, fructan exohydrolase, Fructanase, fructofuranosidase, polysaccharide beta, fructofuranosyl hydrolase, glycoside hydrolase, Ht1-FEH III, inuD, inuE, inulinase, invertase, levanase, Lp6-FEHa, More, P-I, polysaccharide beta-fructofuranosidase, sacCp, Wfh-sm1, Wfh-sm3
ECTree
3.2.1.80 fructan beta-fructosidaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.2.1.80 - fructan beta-fructosidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1F,6G-di-beta-D-fructofuranosylsucrose + H2O
?
1 and 6G-kestotetraose, 57-69% activity compared to neokestose
-
-
?
6G(1-beta-D-fructofuranosyl)2sucrose + H2O
?
i.e. 1,6G-kestotetraose, 14-15% activity compared to neokestose
-
-
?
6G,6-kestotetraose + H2O
D-fructose + neokestose
-
100%, chicorium inulin 11.9%, Aerobacter levan 8.5%, sucrose 0% rate of hydrolysis
-
?
Actilight + H2O
?
-
highest activity, Actilight is a commercial mixture composed of 4.8% (w/v) of free sugars (sucrose, glucose and fructose), 35.1% w/v of 1-kestose, 53% w/v of 1-nystose and 7.1% w/v of 1-fructofuranosyl-nystose
-
-
?
inulobiose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulin is an intermediate product
-
-
?
inuloheptaose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulohexaose, inulopentaose, inulotetraose, inulotriose, inulobiose and inulin are intermediate products
-
-
?
inulohexaose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulopentaose, inulotetraose, inulotriose, inulobiose and inulin are intermediate products
-
-
?
inulopentaose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulotetraose, inulotriose, inulobiose and inulin are intermediate products
-
-
?
inulotetraose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulotriose, inulobiose and inulin are intermediate products
-
-
?
inulotriose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
inulobiose and inulin are intermediate products
-
-
?
levan + H2O
beta-D-fructofuranose + alpha-D-glucopyranose + ?
-
-
-
?
levanohexaose + 6 H2O
6 beta-D-fructofuranose
levanopentaose, levanotetraose, levanotriose, levanobiose and levan are intermediate products
-
-
?
levanopentaose + 4 H2O
5 beta-D-fructofuranose
levanotetraose, levanotriose, levanobiose and levan are intermediate products
-
-
?
levanotetraose + 3 H2O
4 beta-D-fructofuranose
levanotriose, levanobiose and levan are intermediate products
-
-
?
levanotriose + 2 H2O
3 beta-D-fructofuranose
levanobiose and levan are intermediate products
-
-
?
neokestose + H2O
beta-D-fructose + sucrose
-
4% activity of isoform w1 and 3% of w2 in comparison to substrate 1-kestose
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
raffinose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranose
-
-
-
-
?
raffinose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
-
-
-
?
stachyose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-galactopyranosyl-1,6-alpha-D-galactopyranose
-
-
-
-
?
sucrose + H2O
?
0.3% activity compared to 1-kestose
-
-
?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
-
3% enzyme activity in comparison to levan
-
-
?
wheat graminan + H2O
?
recombinant enzyme Wfh-sm3 is able to hydrolyze all components of wheat graminan
-
-
?
wheat graminan + H2O
beta-D-fructose + ?
-
high degree of polymerization
-
-
?
1,1,1-kestose + H2O
beta-D-fructose + sucrose
-
45% enzyme activity in comparison to levan
-
-
?
1,1,1-kestose + H2O
beta-D-fructose + sucrose
-
45% enzyme activity in comparison to levan
-
-
?
1,1-kestose + H2O
beta-D-fructose + sucrose
-
39% enzyme activity in comparison to levan
-
-
?
1,1-kestose + H2O
beta-D-fructose + sucrose
-
39% enzyme activity in comparison to levan
-
-
?
1,1-kestotetraose + H2O
?
-
gene product Wfh-sm1 shows 8% activity with 1,1-kestotetraose compared to sucrose
-
-
?
1,1-nystose + H2O
beta-D-fructose + sucrose
-
72% activity of isoform w1 and 83% of w2 in comparison to substrate 1-kestose
-
-
?
1-kestose + H2O
beta D-fructofuranose + sucrose
-
100% activity of both isoforms w1 and w2
-
-
?
1-kestose + H2O
beta-D-fructose + sucrose
low activity
-
-
?
1-kestose + H2O
beta-D-fructose + sucrose
-
12% enzyme activity in comparison to levan
-
-
?
1-kestose + H2O
beta-D-fructose + sucrose
-
12% enzyme activity in comparison to levan
-
-
?
1-kestotriose + H2O
?
-
gene product Wfh-sm1 shows 40% activity with 1-kestotriose compared to sucrose
-
-
?
1F-fructofuranosylnystose + H2O
?
98% activity compared to 1-kestose
-
-
?
1F-fructofuranosylnystose + H2O
?
99% activity compared to 1-kestose
-
-
?
6-kestose + H2O
beta-D-fructose + sucrose
low activity
-
-
?
6-kestose + H2O
beta-D-fructose + sucrose
-
0.7% activity of isoform w1 and 1% of w2 in comparison to substrate 1-kestose
-
-
?
6-kestotriose + H2O
?
-
gene product Wfh-sm1 shows 38% activity with 6-kestotriose compared to sucrose
-
-
?
6G-kestotriose + H2O
?
19% activity compared to 1,1-kestotetraose
-
-
?
fructosylnystose + H2O
?
35-38% activity compared to neokestose
-
-
?
garlic extract + H2O
?
-
60% enzyme activity in comparison to levan
-
-
?
garlic extract + H2O
?
-
60% enzyme activity in comparison to levan
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
no activity with melizitose
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
no activity with melizitose
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
24% activity compared to 1,1-kestotetraose
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
no activity with bacterial levan
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
no activity with bacterial levan
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
6% activity compared to levan
-
-
?
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
inulin + H2O
?
24-26.5% activity compared to neokestose
-
-
?
inulin + H2O
?
and hydrolysis of inulin-type fructans
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharides are detected, exoinulinase activity
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharides are detected, exoinulinase activity
-
-
?
inulin + H2O
?
25% activity compared to 1-kestose
-
-
?
inulin + H2O
?
31% activity compared to 1-kestose
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharide are produced
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharides are detected after the hydrolysis
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharide are produced
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharides are detected, exoinulinase activity
-
-
?
inulin + H2O
?
-
large amount of monosaccharides and a trace amount of oligosaccharides are detected, exoinulinase activity
-
-
?
inulin + H2O
?
-
gene product Wfh-sm1 shows less than 1% activity with inulin compared to sucrose
-
-
?
inulin + H2O
beta-D-fructose + sucrose + ?
-
9% enzyme activity in comparison to levan
-
-
?
inulin + H2O
D-fructose + ?
-
root powder extract of Asparagus officinalis. The product mainly consists of fructose and traces of glucose
-
-
?
inulin + H2O
D-fructose + ?
-
root powder extract of Asparagus officinalis. The product mainly consists of fructose and traces of glucose
-
-
?
inulin + H2O
D-fructose + ?
-
39% activity of isoform w1 and 22% of w2 in comparison to substrate 1-kestose
-
-
?
inulin + H2O
D-fructose + D-glucose + fructooligosaccharides
-
a typical solution product consists of a mixture of fructose (155 g/l), glucose (155 g/l), sucrose (132 g/l) and fructooligosaccharides (50 g/l)
-
-
?
inulin + H2O
D-fructose + D-glucose + fructooligosaccharides
-
-
-
-
?
inulin + H2O
D-fructose + fructooligosaccharides
-
-
crude inulinase
-
?
levan + H2O
?
2% activity compared to 1-kestose
-
-
?
levan + H2O
?
0.9% activity compared to 1-kestose
-
-
?
levan + H2O
?
-
commercial Erwinia herbicola levan and levan from Leuconostoc mesenteroides DSM 20343-fermented fava bean doughs, combined activity of exoinulinase/levanase
-
-
?
levan + H2O
?
-
gene product Wfh-sm1 shows less than 1% activity with levan compared to sucrose
-
-
?
levan + H2O
D-fructose + ?
0.5% activity compared to 1,1-kestotetraose
-
-
?
levan + H2O
D-fructose + ?
-
4% activity of isoform w1 and 3% of w2 in comparison to substrate 1-kestose
-
-
?
nystose + H2O
?
40-50% activity compared to neokestose
-
-
?
nystose + H2O
?
52% activity compared to 1-kestose
-
-
?
nystose + H2O
?
58% activity compared to 1-kestose
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
-
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
-
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
hydrolyzed at 69% of the activity with inulin
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
hydrolyzed at 69% of the activity with inulin
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
18% of the activity toward inulin
-
-
?
raffinose + H2O
beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
18% of the activity toward inulin
-
-
?
raffinose + H2O
D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
-
-
-
?
raffinose + H2O
D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
-
-
-
?
raffinose + H2O
D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
-
-
-
?
raffinose + H2O
D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
highest activity
-
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
-
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
0.2% activity compared to 1-kestose
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
hydrolyzed at 177.6% of the activity with inulin
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
hydrolyzed at 177.6% of the activity with inulin
-
-
?
sucrose + H2O
D-fructose + D-glucose
-
less than 1%, 2% and 15% activity towards 1 mM, 10 mM and 100 mM sucrose compared to levan
-
-
?
additional information
?
-
-
the enzyme preferentially hydrolyzes the beta-2,1 linkage of terminal fructosyl residue of fructan
-
-
?
additional information
?
-
fructan 6G&1-exohydrolase (6G&1-FEH) hydrolyzes the terminal beta-2,6 and beta-2,1 fructosyl linkages of fructan, substrate specificity, overview. Levan (from Erwinia herbicola) and sucrose are poor substrates. Hydrolysis of inulin-type fructans
-
-
?
additional information
?
-
fructan 6G&1-exohydrolase (6G&1-FEH) hydrolyzes the terminal beta-2,6 and beta-2,1 fructosyl linkages of fructan, substrate specificity, overview. Levan (from Erwinia herbicola) and sucrose are poor substrates. Hydrolysis of inulin-type fructans
-
-
?
additional information
?
-
fructan 6G&1-exohydrolase (6G&1-FEH) hydrolyzes the terminal beta-2,6 and beta-2,1 fructosyl linkages of fructan, substrate specificty, overview
-
-
?
additional information
?
-
fructan 6G&1-exohydrolase (6G&1-FEH) hydrolyzes the terminal beta-2,6 and beta-2,1 fructosyl linkages of fructan, substrate specificty, overview
-
-
?
additional information
?
-
-
the enzyme catalyses the fructan breakdown in plants
-
-
?
additional information
?
-
-
the enzyme is involved in fructan breakdown
-
-
?
additional information
?
-
-
the enzyme is not able to break the alpha (1-2) linkage present in sucrose
-
-
?
additional information
?
-
no hydrolase activity towards sucrose and loliose
-
-
?
additional information
?
-
-
no hydrolase activity towards sucrose and loliose
-
-
?
additional information
?
-
substrate specificity, overview. Among beta(2,6) fructans tested, 6-kestotriose is the best substrate while bacterial levan and 6G-kestotriose are more slowly hydrolyzed. Among beta(2,1) fructans, inulin hydrolysis is neglectable while 1-kestotriose and 1,1-kestotetraose are also hydrolyzed but at a much lower rate than 6-kestotriose, poor activity with sucrose
-
-
?
additional information
?
-
-
substrate specificity, overview. Among beta(2,6) fructans tested, 6-kestotriose is the best substrate while bacterial levan and 6G-kestotriose are more slowly hydrolyzed. Among beta(2,1) fructans, inulin hydrolysis is neglectable while 1-kestotriose and 1,1-kestotetraose are also hydrolyzed but at a much lower rate than 6-kestotriose, poor activity with sucrose
-
-
?
additional information
?
-
substrate specificity, overview. Among beta(2,6) fructans tested, 6-kestotriose is the best substrate while bacterial levan and 6G-kestotriose are more slowly hydrolyzed. Among beta(2,1) fructans, inulin hydrolysis is neglectable while 1-kestotriose and 1,1-kestotetraose are also hydrolyzed but at a much lower rate than 6-kestotriose, poor activity with sucrose
-
-
?
additional information
?
-
-
exo-beta-fructosidases, including exoinulinases (EC 3.2.1.80), can nonspecifically hydrolyze both beta-(2->1) and beta-(2->6)-linkages from the non-reducing end of fructooligosaccharides and fructans. In order to develop a specific analysis for levan in food matrices, a Paenibacillus amylolyticus endolevanase (EC 3.2.1.65) is combined with exoinulinase for levan hydrolysis achieving about 80% hydrolysis yield (19.1% for exoinulinase alone), method evaluation, and modelling. The levanase preparation exhibits an activity of 1116 U/ml towards levan and no activity towards inulin as determined by the reducing value method
-
-
?
additional information
?
-
-
the recombinant enzyme completely degrades fructans that are composed mainly of beta(2,6)-linked and linear fructans (levan) with a high degree of polymerization. The enzyme has significant activity against beta(2,1)-linked fructans, but considerably less than against beta(2,6)-linked fructans. The enzyme also has weak invertase activity
-
-
?
additional information
?
-
-
not: melizitose, dextran, pseudonigeran
-
-
?
additional information
?
-
-
the enzyme shows invertase activity releasing fructose and glucose from sucrose, EC 3.2.1.26
-
-
?
additional information
?
-
-
the enzyme might play a role as a beta-(2,1)-trimmer throughout the period of active graminan
-
-
?
additional information
?
-
-
no activity towards sucrose and high degree of polymerization-inulin
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-
?
additional information
?
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no activity towards sucrose, 1-kestose, nystose, inulin
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-
?
