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Literature summary for 3.2.1.80 extracted from
- Purification and characterisation of an extracellular fructan beta-fructosidase from a Lactobacillus pentosus strain isolated from fermented fish (2002), Syst. Appl. Microbiol., 25, 13-20.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Lactiplantibacillus pentosus |  |
| Cu2+ | - |
Lactiplantibacillus pentosus | |
| EDTA | - |
Lactiplantibacillus pentosus | |
| Fe2+ | - |
Lactiplantibacillus pentosus | |
| p-chloromercuribenzoate | - |
Lactiplantibacillus pentosus | |
| Zn2+ | - |
Lactiplantibacillus pentosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 125000 | - |
1 * 125000, SDS-PAGE | Lactiplantibacillus pentosus |
| 126000 | - |
gel filtration | Lactiplantibacillus pentosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus pentosus | - |
B235 | - |
| Lactiplantibacillus pentosus B235 | - |
B235 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 261 | - |
- |
Lactiplantibacillus pentosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,1,1-kestose + H2O | 45% enzyme activity in comparison to levan | Lactiplantibacillus pentosus | beta-D-fructose + sucrose | - |
? | |
| 1,1,1-kestose + H2O | 45% enzyme activity in comparison to levan | Lactiplantibacillus pentosus B235 | beta-D-fructose + sucrose | - |
? | |
| 1,1-kestose + H2O | 39% enzyme activity in comparison to levan | Lactiplantibacillus pentosus | beta-D-fructose + sucrose | - |
? | |
| 1,1-kestose + H2O | 39% enzyme activity in comparison to levan | Lactiplantibacillus pentosus B235 | beta-D-fructose + sucrose | - |
? | |
| 1-kestose + H2O | 12% enzyme activity in comparison to levan | Lactiplantibacillus pentosus | beta-D-fructose + sucrose | - |
? | |
| 1-kestose + H2O | 12% enzyme activity in comparison to levan | Lactiplantibacillus pentosus B235 | beta-D-fructose + sucrose | - |
? | |
| garlic extract + H2O | 60% enzyme activity in comparison to levan | Lactiplantibacillus pentosus | ? | - |
? | |
| garlic extract + H2O | 60% enzyme activity in comparison to levan | Lactiplantibacillus pentosus B235 | ? | - |
? | |
| inulin + H2O | 9% enzyme activity in comparison to levan | Lactiplantibacillus pentosus | beta-D-fructose + sucrose + ? | - |
? | |
| levan + H2O | 100% enzyme activity | Lactiplantibacillus pentosus | D-fructose + ? | - |
? | |
| levan + H2O | 100% enzyme activity | Lactiplantibacillus pentosus B235 | D-fructose + ? | - |
? | |
| sucrose + H2O | 3% enzyme activity in comparison to levan | Lactiplantibacillus pentosus | alpha-D-glucose + beta-D-fructose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 125000, SDS-PAGE | Lactiplantibacillus pentosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fructan beta-fructosidase | - |
Lactiplantibacillus pentosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Lactiplantibacillus pentosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 35 | 65% of maximal activity at 15°C, 70% of maximal activity at 35°C | Lactiplantibacillus pentosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
more than 70% of maximal activity at pH 5.8 after 24 h | Lactiplantibacillus pentosus |
| 15 | - |
more than 70% of maximal activity at pH 5.8 after 24 h | Lactiplantibacillus pentosus |
| 25 | - |
more than 70% of maximal activity at pH 5.8 after 24 h | Lactiplantibacillus pentosus |
| 37 | - |
more than 70% of maximal activity at pH 5.8 after 24 h | Lactiplantibacillus pentosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Lactiplantibacillus pentosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | 85% of maximal activity at both pH 4.0 and 6.0 | Lactiplantibacillus pentosus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
55% of maximal activity | Lactiplantibacillus pentosus |
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