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3.2.1.45: glucosylceramidase

This is an abbreviated version!
For detailed information about glucosylceramidase, go to the full flat file.

Word Map on EC 3.2.1.45

Reaction

a D-glucosyl-N-acylsphingosine
+
H2O
=
D-glucose
 +
a ceramide

Synonyms

ABG, acid beta-D-glucosidase, acid beta-glucocerebrosidase, acid beta-glucosidase, acid beta-glucosylceramidase, acid beta-glycosidase, acid glucosylceramidase, acid-beta-glucocerebrosidase, acid-beta-glucosidase, Alglucerase, beta-D-glucocerebrosidase, beta-GC, beta-glucocerebrosidase, beta-glucosidase, beta-glucosidase 2, beta-glucosylceramidase, beta-glucosylceramidase 2, beta-glycosylceramidase, bile acid beta-glucosidase, ceramidase, glucosyl-, ceramide glucosidase, cerebroside beta-glucosidase, Ceredase, Cerezyme, D-glucosyl-N-acylsphingosine glucohydrolase, GBA, GBA1, GBA2, Gcase, GCD, GCR, GDA, gene-activated human GlcCerase, Glc-Cerase, glcCer-beta-glucosidase, GlcCerase, glucocerebrosidase, glucocerebroside beta-glucosidase, glucose cerebrosidase, glucosphingosine glucosylhydrolase, glucosylceramidase, glucosylceramide beta-glucosidase, glucosylcerebrosidase, glucosylsphingosine beta-D-glucosidase, glucosylsphingosine beta-glucosidase, glycosylceramidase, glycosylceramide-beta-glucosidase, Imiglucerase, Klotho-related protein, KLrP, lysosomal glucosylceramidase, microsomal bile acid beta-glucosidase, N-acylsphingosyl-1-O-beta-D-glucoside:glucohydrolase, neutral beta-glycosylceramidase, neutral glucosylceramidase, NLGase, non-lysosomal beta-glucosylceramidase, non-lysosomal glucosylceramidase, nonlysosomal beta-glucosidase, nonlysosomal glucosylceramidase, psychosine hydrolase, taliglucerase alfa, velaglucerase alfa, Vpriv

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.45 glucosylceramidase

Cloned

Cloned on EC 3.2.1.45 - glucosylceramidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
alterations in enzyme gene are identified in 12 of the 57 subjects with Parkinson`s disease
-
comparative and genetic analysis of the porcine glucocerebrosidase gene
expressed in Arabidopsis thaliana wild-type (Col-0) seeds
-
expressed in Chinese hamster ovary cells
-
expressed in Chinese hamster ovary cells and in transgenic carrot cells
expressed in CHO cells
expressed in CHO cells and Tn-5 cells
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expressed in COS-7 cells
expressed in cultured plant cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Mus musculus liver
-
expressed in Nicotiana benthamiana
expressed in Nicotiana tabacum cultivar Bright Yellow 2 cells
-
expressed in Sf9 insect cells
-
expressed in transgenic rice calli (Oryza sativa cultivar Dongjin) and Chinese hamster ovary cells
-
expression in Escherichia coli
expression in mouse fibroblasts NIH3T3
-
expression in NIH 3T3 cells
expression in Sf9 cells
expression of mutant and wild-type enzymes in murine cells
-
expression of wild-type and mutant enzymes by vaccinia based system, the wild-type and mutant enzymes are expressed in Gaucher fibroblast cell line L444P/S107L, HeLa G and BSC40, only enzyme-deficient fibroblasts are suitable for expression using plasmid transfection
-
gene-activated GCase is produced in an immortalized human cell line (HT-1080) utilizing targeted recombination with a promoter that activates the endogenous GCase in the presence of the mannosidase I inhibitor kifunensine, which results in a molecule with predominantly high-mannose-type glycans
-
overexpression in CHO cells, transfected with the tetracycline transactivation conditional expression system, majority of enzyme is secreted and overexpression in C2C12 cells, transducted with MFG-enzyme retrovirus
-
recombinant glucocerebrosidase uptake by Gaucher disease human osteoblast culture model
-
the mutated gene is subcloned into the mammalian expression vector pCR(R) 3.1 and expressed by transient transfection in COS cells
using fragments of the enzyme cDNA fused to the luciferase c DNA as a translational read-through reporter, the impact of synonymous codon usage bias on protein expression in Pichia pastoris as a potential system for the high-level heterologous production of human enzyme
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