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3.2.1.45: glucosylceramidase

This is an abbreviated version!
For detailed information about glucosylceramidase, go to the full flat file.

Word Map on EC 3.2.1.45

Reaction

a D-glucosyl-N-acylsphingosine
+
H2O
=
D-glucose
 +
a ceramide

Synonyms

ABG, acid beta-D-glucosidase, acid beta-glucocerebrosidase, acid beta-glucosidase, acid beta-glucosylceramidase, acid beta-glycosidase, acid glucosylceramidase, acid-beta-glucocerebrosidase, acid-beta-glucosidase, Alglucerase, beta-D-glucocerebrosidase, beta-GC, beta-glucocerebrosidase, beta-glucosidase, beta-glucosidase 2, beta-glucosylceramidase, beta-glucosylceramidase 2, beta-glycosylceramidase, bile acid beta-glucosidase, ceramidase, glucosyl-, ceramide glucosidase, cerebroside beta-glucosidase, Ceredase, Cerezyme, D-glucosyl-N-acylsphingosine glucohydrolase, GBA, GBA1, GBA2, Gcase, GCD, GCR, GDA, gene-activated human GlcCerase, Glc-Cerase, glcCer-beta-glucosidase, GlcCerase, glucocerebrosidase, glucocerebroside beta-glucosidase, glucose cerebrosidase, glucosphingosine glucosylhydrolase, glucosylceramidase, glucosylceramide beta-glucosidase, glucosylcerebrosidase, glucosylsphingosine beta-D-glucosidase, glucosylsphingosine beta-glucosidase, glycosylceramidase, glycosylceramide-beta-glucosidase, Imiglucerase, Klotho-related protein, KLrP, lysosomal glucosylceramidase, microsomal bile acid beta-glucosidase, N-acylsphingosyl-1-O-beta-D-glucoside:glucohydrolase, neutral beta-glycosylceramidase, neutral glucosylceramidase, NLGase, non-lysosomal beta-glucosylceramidase, non-lysosomal glucosylceramidase, nonlysosomal beta-glucosidase, nonlysosomal glucosylceramidase, psychosine hydrolase, taliglucerase alfa, velaglucerase alfa, Vpriv

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.45 glucosylceramidase

Specific Activity

Specific Activity on EC 3.2.1.45 - glucosylceramidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00007
-
mutant E235A
0.00008
-
mutant E340A
0.0046
-
wild-type enzyme
0.02
-
expressed activity of wild-type enzyme
0.075 - 0.1
-
enzyme in cell lines from two patients with Niemann-Pick disease type C
0.0817
with methyl beta-D-glucoside as substrate, wild type enzyme, at pH 5.5 and 60°C
0.123
with 4-nitrophenyl N-acetyl-beta-D-glucosaminide as substrate, wild type enzyme, at pH 5.5 and 60°C
0.4
-
enzyme in normal fibroblasts
11.7
with laminaribiose as substrate, wild type enzyme, at pH 5.5 and 60°C
13.4
with cellobiose as substrate, wild type enzyme, at pH 5.5 and 60°C
13.5
with cellohexaose as substrate, wild type enzyme, at pH 5.5 and 60°C
13.8
with cellopentaose as substrate, wild type enzyme, at pH 5.5 and 60°C
14.9
with cellotetraose as substrate, wild type enzyme, at pH 5.5 and 60°C
15
with laminaritetraose as substrate, wild type enzyme, at pH 5.5 and 60°C
15.6
with cellotriose as substrate, wild type enzyme, at pH 5.5 and 60°C
16.1
with laminaritriose as substrate, wild type enzyme, at pH 5.5 and 60°C
16.5
with laminaripentaose as substrate, wild type enzyme, at pH 5.5 and 60°C
16.6
-
-
2.23
with gentibiose as substrate, wild type enzyme, at pH 5.5 and 60°C
26.7
-
-
27.3
with 4-nitrophenyl beta-D-glucoside as substrate, wild type enzyme, at pH 5.5 and 60°C
3.57
-
-
3.58
with n-octyl beta-D-glucoside as substrate, wild type enzyme, at pH 5.5 and 60°C
3.74
with n-heptyl beta-D-glucoside as substrate, wild type enzyme, at pH 5.5 and 60°C
4.1
with sophorose as substrate, wild type enzyme, at pH 5.5 and 60°C
8.9
with 4-nitrophenyl beta-D-galactoside as substrate, wild type enzyme, at pH 5.5 and 60°C
additional information