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3.2.1.45: glucosylceramidase

This is an abbreviated version!
For detailed information about glucosylceramidase, go to the full flat file.

Word Map on EC 3.2.1.45

Reaction

a D-glucosyl-N-acylsphingosine
+
H2O
=
D-glucose
 +
a ceramide

Synonyms

ABG, acid beta-D-glucosidase, acid beta-glucocerebrosidase, acid beta-glucosidase, acid beta-glucosylceramidase, acid beta-glycosidase, acid glucosylceramidase, acid-beta-glucocerebrosidase, acid-beta-glucosidase, Alglucerase, beta-D-glucocerebrosidase, beta-GC, beta-glucocerebrosidase, beta-glucosidase, beta-glucosidase 2, beta-glucosylceramidase, beta-glucosylceramidase 2, beta-glycosylceramidase, bile acid beta-glucosidase, ceramidase, glucosyl-, ceramide glucosidase, cerebroside beta-glucosidase, Ceredase, Cerezyme, D-glucosyl-N-acylsphingosine glucohydrolase, GBA, GBA1, GBA2, Gcase, GCD, GCR, GDA, gene-activated human GlcCerase, Glc-Cerase, glcCer-beta-glucosidase, GlcCerase, glucocerebrosidase, glucocerebroside beta-glucosidase, glucose cerebrosidase, glucosphingosine glucosylhydrolase, glucosylceramidase, glucosylceramide beta-glucosidase, glucosylcerebrosidase, glucosylsphingosine beta-D-glucosidase, glucosylsphingosine beta-glucosidase, glycosylceramidase, glycosylceramide-beta-glucosidase, Imiglucerase, Klotho-related protein, KLrP, lysosomal glucosylceramidase, microsomal bile acid beta-glucosidase, N-acylsphingosyl-1-O-beta-D-glucoside:glucohydrolase, neutral beta-glycosylceramidase, neutral glucosylceramidase, NLGase, non-lysosomal beta-glucosylceramidase, non-lysosomal glucosylceramidase, nonlysosomal beta-glucosidase, nonlysosomal glucosylceramidase, psychosine hydrolase, taliglucerase alfa, velaglucerase alfa, Vpriv

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.45 glucosylceramidase

General Stability

General Stability on EC 3.2.1.45 - glucosylceramidase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
chemical chaperones stabilize Gaucher disease-associated glucocerebroside variants N370S and G202R against misfolding, enabling their trafficking from the endoplasmic reticulum. The L44P variant is not chaperoned, likely because this mutation destabilizes a domain distinct from the catalytic domain
-
cyclophellitol-derived activity-based probes improve the stability of the enzyme in vitro. At pH 5.2, the recombinant enzyme shows resistance to tryptic digestion over the course of 90 min, whereas at pH 7.4, the enzyme is more sensitive to trypsin digestion, with about 40% degraded within 60 min
dialysis, stable to
-
dithiothreitol stabilizes in later stages of chromatographic separation
-
freezing destroys activity
glycerol stabilizes in later stages of chromatographic separation
-
phospholipid, preincubation stabilizes
-