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Literature summary for 3.2.1.45 extracted from

  • Brumshtein, B.; Salinas, P.; Peterson, B.; Chan, V.; Silman, I.; Sussman, J.L.; Savickas, P.J.; Robinson, G.S.; Futerman, A.H.
    Characterization of gene-activated human acid-beta-glucosidase: Crystal structure, glycan composition, and internalization into macrophages (2010), Glycobiology, 20, 24-32.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.45

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Chinese hamster ovary cells and in transgenic carrot cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
velaglucerase alfa, micro-batch crystallization under oil, using 1 M (NH4)2SO4/0.1 M HEPES, pH 7.0, containing 0.5% (w/v) PEG8000 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 glucosylceramide imiglucerase, in 50 mM sodium citrate, pH 6.0 with 0.75 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.019
-
 glucosylceramide velaglucerase alfa, in 50 mM sodium citrate, pH 6.0 with 0.75 mg/ml bovine serum albumin, at 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P04062
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucosylceramide + H2O
-
 Homo sapiens beta-D-glucose + ceramide
-
 ?

Synonyms

Synonyms Comment Organism
acid-beta-glucocerebrosidase
-
 Homo sapiens
acid-beta-glucosidase
-
 Homo sapiens
Cerezyme commercial preparation Homo sapiens
gene-activated human GlcCerase commercial preparation Homo sapiens
GlcCerase
-
 Homo sapiens
Imiglucerase
-
 Homo sapiens
velaglucerase alfa commercial preparation Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
31.67
-
 glucosylceramide imiglucerase alfa, in 50 mM sodium citrate, pH 6.0 with 0.75 mg/ml bovine serum albumin, at 37°C Homo sapiens
35
-
 glucosylceramide velaglucerase alfa, in 50 mM sodium citrate, pH 6.0 with 0.75 mg/ml bovine serum albumin, at 37°C Homo sapiens