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Literature summary for 3.2.1.45 extracted from

  • Hayashi, Y.; Okino, N.; Kakuta, Y.; Shikanai, T.; Tani, M.; Narimatsu, H.; Ito, M.
    Klotho-related protein is a novel cytosolic neutral beta-glycosylceramidase (2007), J. Biol. Chem., 282, 30889-30900.
    View publication on PubMed
Search for more literature for 3.2.1.45

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with 0.1 M Tris-HCl buffer, pH 8.5, containing 0.2 M magnesium chloride, 27.5% PEG 3350, 5% glycerol, 0.5 M glucose or 0.5 M galactose Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00204
-
 (6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)-beta-D-galactosylceramide in 50 mM MES buffer, pH 6.0, at 37°C Homo sapiens
0.00464
-
 (6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide in 50 mM MES buffer, pH 6.0, at 37°C Homo sapiens
0.00924
-
 C18-galactosylceramide in 50 mM MES buffer, pH 6.0, at 37°C Homo sapiens
0.01367
-
 C18-glucosylceramide in 50 mM MES buffer, pH 6.0, at 37°C Homo sapiens
0.04928
-
 4-methylumbelliferyl-beta-D-glucopyranoside in 50 mM MES buffer, pH 6.0, at 37°C Homo sapiens
0.1445
-
 4-methylumbelliferyl-beta-D-galactopyranoside in 50 mM MES buffer, pH 6.0, at 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Hi-Trap Chelating HP column chromatography and Superdex 200 10/300 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide + H2O
-
 Homo sapiens (6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)sphingosine + D-glucose
-
 ?
4-methylumbelliferyl-beta-D-galactopyranoside + H2O
-
 Homo sapiens methylumbelliferone + beta-D-galactose
-
 ?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Homo sapiens methylumbelliferone + beta-D-glucose
-
 ?
6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)-beta-D-galactosylceramide + H2O
-
 Homo sapiens 6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)ceramide + D-galactose
-
 ?
C18-galactosylceramide + H2O
-
 Homo sapiens ?
-
 ?
C18-glucosylceramide + H2O
-
 Homo sapiens ?
-
 ?
glucocerebroside + H2O
-
 Homo sapiens beta-D-glucose + ceramide
-
 ?

Synonyms

Synonyms Comment Organism
beta-glycosylceramidase
-
 Homo sapiens
Gcase
-
 Homo sapiens
Klotho-related protein
-
 Homo sapiens
KLrP
-
 Homo sapiens
neutral beta-glycosylceramidase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
 C18-glucosylceramide in 50 mM MES buffer, pH 6.0, at 37°C Homo sapiens
0.03
-
 (6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)-beta-D-galactosylceramide in 50 mM MES buffer, pH 6.0, at 37°C Homo sapiens
0.12
-
 (6-[N-(7-nitrobenz-2-oxa-1,3 diazol-4-yl)amino]hexanoyl)-beta-D-glucosylceramide in 50 mM MES buffer, pH 6.0, at 37°C Homo sapiens
1.23
-
 4-methylumbelliferyl-beta-D-glucopyranoside in 50 mM MES buffer, pH 6.0, at 37°C Homo sapiens
1.57
-
 4-methylumbelliferyl-beta-D-galactopyranoside in 50 mM MES buffer, pH 6.0, at 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Homo sapiens