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Literature summary for 3.2.1.45 extracted from

  • Sumida, T.; Sueyoshi, N.; Ito, M.
    Molecular cloning and characterization of a novel glucocerebrosidase of Paenibacillus sp. TS12 (2002), J. Biochem., 132, 237-243.
    View publication on PubMed
Search for more literature for 3.2.1.45

Activating Compound

Activating Compound Comment Organism Structure
Lubrol Px activates when C12-4-nitrobenz-2-oxa-1,3-diazole labelled glucosylceramide is substrate at optimal concentration of 0.05-0.15% Paenibacillus sp.
sodium cholate activates when C12-4-nitrobenz-2-oxa-1,3-diazole labelled glucosylceramide is substrate at concentration of optimal 0.5% Paenibacillus sp.
Triton X-100 activates when C12-4-nitrobenz-2-oxa-1,3-diazole labelled glucosylceramide is substrate at optimal concentration of 0.05-0.15% Paenibacillus sp.
Tween 20 activates when C12-4-nitrobenz-2-oxa-1,3-diazole labelled glucosylceramide is substrate at optimal concentration of 0.05-0.15% Paenibacillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paenibacillus sp.

Protein Variants

Protein Variants Comment Organism
D223E mutation decreases the activity Paenibacillus sp.
D223N mutation completely abolishes the activity Paenibacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ strong inhibition at 5 mM Paenibacillus sp.
Co2+ complete inhibition at 5 mM Paenibacillus sp.
Cu2+ complete inhibition at 5 mM Paenibacillus sp.
Hg2+ complete inhibition at 5 mM Paenibacillus sp.
Mg2+ strong inhibition at 5 mM Paenibacillus sp.
Mn2+ strong inhibition at 5 mM Paenibacillus sp.
Ni2+ complete inhibition at 5 mM Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.223
-
 glucosylceramide pH 6.5, 37°C Paenibacillus sp.
0.593
-
 p-nitrophenyl-beta-glucopyranoside pH 6.5, 37°C, wild-type enzyme Paenibacillus sp.
1.35
-
 p-nitrophenyl-beta-glucopyranoside pH 6.5, 37°C, mutant D223E Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90700
-
 x * 90700, SDS-PAGE Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp.
-
 TS12
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucosylceramide + H2O
-
 Paenibacillus sp. ?
-
 ?
additional information not: galactosylceramide, GM1a and sphingomyelin Paenibacillus sp. ?
-
 ?
p-nitrophenyl beta-D-glucopyranoside + H2O
-
 Paenibacillus sp. p-nitrophenol + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 90700, SDS-PAGE Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Paenibacillus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Paenibacillus sp.
-
-
 4.9