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3.2.1.25: beta-mannosidase

This is an abbreviated version!
For detailed information about beta-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.25

Reaction

Manbeta1-4GlcNAcbeta(1-4)GlcNAcbeta-Asn
+
H2O
=
GlcNAc-(1-4)-beta-GlcNAc-Asn
+
beta-D-mannopyranose

Synonyms

beta-D-mannosidase, beta-MAN, beta-mannosidase, beta-mannosidase 2A, beta-mannoside mannohydrolase, betaMANNOS1, BglB, CmMan5, CmMan5a, exo beta-mannanase, exo-beta-D-mannanase, GH2 beta-mannosidase, GM-1, GM-2, HvBII, Man2A, ManB, mannanase, mannase, mannosidase 5A, mannosidase, beta-, OT-1, PH0501, TM1624

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.25 beta-mannosidase

Molecular Weight

Molecular Weight on EC 3.2.1.25 - beta-mannosidase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10000
-
gel filtration
100000
100300
-
isozyme OT-1, SDS-PAGE
104200
-
calculated from amino acid sequence
104600
-
isozyme GM-1, SDS-PAGE
105000
-
x * 105000, SDS-PAGE
110000
-
gel filtration
114000
-
x * 114000, SDS-PAGE
120000
-
gel filtration
130000
135000
137000
-
beta-mannosidase A, enzyme from kidney, gradient gel electrophoresis
140000
155000
-
beta-mannosidase B, enzyme from kidney, gradient gel electrophoresis
160000
-
beta-mannosidase B, enzyme from urine, nondenaturing gradient PAGE
180000
-
gel filtration
193000
-
gel filtration
200000
250000
gel filtration
36000
x * 36000, SDS-PAGE of catalytic domain
37000
-
1 * 37000 + 1 * 49000 + 1 * 75000, beta-mannosidase A, SDS-PAGE
38000
-
beta-mannanase II, disc gel electrophoresis
43000
-
beta-mannanase I, disc gel electrophoresis
49000
54000
56000
-
x * 56000, SDS-PAGE
56457
4 * 56457, calculated from sequence
56500
4 * 56500, SDS-PAGE
57500
-
x * 57500, SDS-PAGE
59000
-
gel filtration
64000
Polyporus sulfureus
-
gel filtration
75000
79000
-
gel filtration
88000
-
x * 88000, SDS-PAGE
89500
-
isozyme GM-2, SDS-PAGE
93200
gel filtration
94000
95000
-
2 * 95000, SDS-PAGE
97000
x * 97000, SDS-PAGE
99900
-
SDS-PAGE