3.2.1.25: beta-mannosidase

This is an abbreviated version!
For detailed information about beta-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.25

3.2.1.25

lysosomal

oligosaccharide

alpha-mannosidase

goat

alpha-galactosidase

glycosidases

caprine

mannans

galactomannans

alpha-fucosidase

beta-hexosaminidase

beta-xylosidase

salers

nubian

1-4glcnac

beta-fucosidase

beta-glycosidases

angiokeratomas

beta-d-glucosidase

medicine

analysis

Reaction

Synonyms

beta-D-mannosidase, beta-MAN, beta-mannosidase, beta-mannosidase 2A, beta-mannoside mannohydrolase, betaMANNOS1, BglB, CmMan5, CmMan5a, exo beta-mannanase, exo-beta-D-mannanase, GH2 beta-mannosidase, GM-1, GM-2, HvBII, Man2A, ManB, mannanase, mannase, mannosidase 5A, mannosidase, beta-, OT-1, PH0501, TM1624

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.25 beta-mannosidase

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Results	in table
16	Activating Compound
4311	AA Sequence
4	Application
1	CAS Registry Number
15	Cloned(Commentary)
8	Crystallization (Commentary)
296	Disease/ Diagnostics
2	Expression
2	General Information
7	General Stability
97	Inhibitors
20	kcat/KM [mM/s]
24	Ki Value [mM]
92	KM Value [mM]
15	Localization
5	Metals/Ions
52	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
114	Organism
1	Oxidation Stability
1	Pathway
35	PDB ID
38	pH Optimum
6	pH Range
20	pH Stability
7	pI Value
14	Posttranslational Modification
33	Protein Variants
37	Purification (Commentary)
2	Reaction
1	Reaction Type
91	Reference
52	Source Tissue
32	Specific Activity [micromol/min/mg]
6	Storage Stability
197	Substrates and Products (Substrate)
35	Subunits
36	Synonyms
1	Systematic Name
20	Temperature Optimum [°C]
6	Temperature Range [°C]
40	Temperature Stability [°C]
45	Turnover Number [1/s]

Cloned

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Cloned on EC 3.2.1.25 - beta-mannosidase

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2 entries

expressed as a soluble C-terminal His-tagged protein in Escherichia coli

Thermotoga maritima

Q9X1V9

748422

expressed in Aspergillus aculeatus strain PyrG

Aspergillus aculeatus

681713

expressed in Escherichia coli strain BL21

Bacteroides thetaiotaomicron

680752

expression in Escherichia coli

5 entries

expression in HEK-293T cell

Homo sapiens

707885

expression of catalytic domain in Escherichia coli

Bacillus sp. (in: Bacteria)

Q5YEX6

695365

man2A gene is subcloned into the expression vector pET28A(+), yielding the plasmid pET28Man. The protein encoded by this plasmid carries a C-terminal His6 tag for purification, expression in Escherichia coli BL21

Cellulomonas fimi

654244

the gene encoding beta-mannosidase is localized to human chromosome 4

Bos taurus

136053

wild type and mutant enzymes Q77R, D206N, D206Q, E207S and E399S are expressed in Escherichia coli

Thermococcus kodakarensis

Q9YGB8

750443