Cloned (Comment) | Organism |
---|---|
man2A gene is subcloned into the expression vector pET28A(+), yielding the plasmid pET28Man. The protein encoded by this plasmid carries a C-terminal His6 tag for purification, expression in Escherichia coli BL21 | Cellulomonas fimi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-nitrophenyl-beta-mannoside | above 0.4 mM | Cellulomonas fimi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | as the enzyme is inhibited by the substrate p-nitrophenyl-beta-mannoside at concentrations above 0.4 mM, the KM-value of 0.3 mM is only an estimate | Cellulomonas fimi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Cellulomonas fimi | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
gel filtration | Cellulomonas fimi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas fimi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no hydrolysis of p-nitrophenyl derivatives of alpha-mannose, beta-N-acetylglucosamine, beta-glucose, beta-xylose, beta-cellobiose or beta-gentiobiose | Cellulomonas fimi | ? | - |
? | |
p-nitrophenyl-beta-galactoside + H2O | low activity | Cellulomonas fimi | p-nitrophenol + beta-D-galactopyranose | - |
? | |
p-nitrophenyl-beta-mannoside + H2O | - |
Cellulomonas fimi | p-nitrophenol + beta-D-mannopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-mannosidase 2A | - |
Cellulomonas fimi |
Man2A | - |
Cellulomonas fimi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Cellulomonas fimi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life: 27 h | Cellulomonas fimi |
55 | - |
unstable at | Cellulomonas fimi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | as the enzyme is inhibited by the substrate p-nitrophenyl-beta-mannoside at concentrations above 0.4 mM, the turnover number of 167 per s is only an estimate | Cellulomonas fimi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Cellulomonas fimi |