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3.2.1.25: beta-mannosidase

This is an abbreviated version!
For detailed information about beta-mannosidase, go to the full flat file.

Word Map on EC 3.2.1.25

Reaction

Manbeta1-4GlcNAcbeta(1-4)GlcNAcbeta-Asn
+
H2O
=
GlcNAc-(1-4)-beta-GlcNAc-Asn
 +
beta-D-mannopyranose

Synonyms

beta-D-mannosidase, beta-MAN, beta-mannosidase, beta-mannosidase 2A, beta-mannoside mannohydrolase, betaMANNOS1, BglB, CmMan5, CmMan5a, exo beta-mannanase, exo-beta-D-mannanase, GH2 beta-mannosidase, GM-1, GM-2, HvBII, Man2A, ManB, mannanase, mannase, mannosidase 5A, mannosidase, beta-, OT-1, PH0501, TM1624

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.25 beta-mannosidase

Inhibitors

Inhibitors on EC 3.2.1.25 - beta-mannosidase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(5R,6R,7S,8S)-5-(hydroxymethyl)-2-phenyl-5,6,7,8-tetrahydroimidazol[1,2-a]pyridine-6,7,8-triol
-
(5S,6R,8R)-5-(hydroxymethyl)-2-(2-phenylethyl)-5,6,7,8-tetrahydroimidazo[1,2-a]pyridine-6,7,8-triol
persuasive transition state mimic
(5S,6R,8R)-5-(hydroxymethyl)-2-(phenoxymethyl)-5,6,7,8-tetrahydroimidazo[1,2-a]pyridine-6,7,8-triol
persuasive transition state mimic
(5S,6R,8R)-5-(hydroxymethyl)-2-[(phenylamino)methyl]-5,6,7,8-tetrahydroimidazo[1,2-a]pyridine-6,7,8-triol
persuasive transition state mimic
2-amino-2-deoxy-D-mannose
2-deoxy-2-fluoro-beta-D-mannosyl fluoride
-
time-dependent inactivation through accumulation of a covalent 2-deoxy-2-fluoro-alpha-D-mannosyl-beta-mannosidase 2A enzyme intermediate
2-deoxy-2-fluoro-beta-glycosyl fluorides
-
in vivo inhibition
-
4-nitrophenyl beta-D-thioglucoside
competitive, adopts 4NP-S-Glc 3S5 or 1S3 conformations upon binding according to STD NMR and trNOESY experiments. QM modeling and docking, based on GLIDE scores, predicts that 4-nitrophenyl beta-D-thioglucoside preferentially binds in 1S3 geometries
4-nitrophenyl beta-D-thiomannoside
competitive, adopts 4C1 geometry upon binding according to STD NMR and trNOESY experiments. QM modeling and docking, based on GLIDE scores, predicts that 4-nitrophenyl beta-D-thiomannoside preferentially binds in 1S3 geometries
beta-1,4-mannooligosaccharides
-
inhibit binding of the enzyme to galactomannans
-
Ca2+
-
5 mM Ca(NO3)2, 45% inhibition
D-mannono-1,4-lactone
-
-
D-mannosamine
-
-
D-mannose
dimethyl sulfoxide
5 mM, no residual activity
dimethylformamide
5 mM, no residual activity
ethanol
5 mM, no residual activity
Fe3+
Polyporus sulfureus
-
-
guanidine hydrochloride
-
0.6 M, 30% inhibition
iodoacetate
5 mM, no residual activity
isofagomine lactam
-
-
mannose
-
relative inhibition of 20%, three concentrations of 10, 50, and 100 mM tested
Mg2+
1 mM, 4% loss of activity
Mn2+
1 mM, 4% loss of activity
monoiodoacetate
-
-
N,N-(2-aminoethyl)-D-glucoamidine
-
-
N,N-(2-aminoethyl)-D-mannoamidine
-
-
N,N-(3-aminopropyl)-D-glucoamidine
-
-
N,N-(3-aminopropyl)-D-mannoamidine
-
-
N,N-(3-hydroxypropyl)-D-glucoamidine
-
-
N,N-(3-hydroxypropyl)-D-mannoamidine
-
-
N,N-(4-aminobutyl)-D-glucoamidine
-
-
N,N-(4-aminobutyl)-D-mannoamidine
-
-
N,N-propyl-D-mannoamidine
-
-
N-bromosuccinimide
-
-
n-Dodecylbenzene sulfonate
-
-
N-propyl-D-glucoamidine
-
-
N-[(2Z,3R,5R,6S)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-ylidene]ethane-1,2-diaminium
-
N-[(2Z,3R,5R,6S)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-ylidene]propan-1-aminium
-
p-nitrophenyl-alpha-D-mannopyranoside
p-nitrophenyl-beta-mannoside
-
above 0.4 mM
Sodium dodecyl sulfate
5 mM, no residual activity