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Literature summary for 3.2.1.25 extracted from

  • McCleary, B.V.
    exo-beta-D-Mannanase (1988), Methods Enzymol., 160, 589-595.
No PubMed abstract available
Search for more literature for 3.2.1.25

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Cyamopsis tetragonoloba
Cu2+
-
 Cyamopsis tetragonoloba
D-mannose 10 mM Cyamopsis tetragonoloba
Hg2+
-
 Cyamopsis tetragonoloba
Zn2+
-
 Cyamopsis tetragonoloba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 p-nitrophenyl-beta-D-mannopyranoside
-
 Cyamopsis tetragonoloba
0.5
-
 4-methylumbelliferyl beta-D-mannopyranoside
-
 Cyamopsis tetragonoloba
2.8
-
 Beta-D-mannohexaitol
-
 Cyamopsis tetragonoloba
2.8
-
 beta-D-mannopentaitol
-
 Cyamopsis tetragonoloba
3
-
 o-nitrophenyl-beta-D-mannopyranoside
-
 Cyamopsis tetragonoloba
12
-
 beta-D-mannotetraitol
-
 Cyamopsis tetragonoloba
80
-
 beta-D-mannotriitol
-
 Cyamopsis tetragonoloba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 gel filtration Cyamopsis tetragonoloba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Cyamopsis tetragonoloba the enzyme plays a key role in the hydrolysis of galactomannan in germinating guar seed ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cyamopsis tetragonoloba
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 7% carbohydrate Cyamopsis tetragonoloba

Purification (Commentary)

Purification (Comment) Organism
-
 Cyamopsis tetragonoloba

Source Tissue

Source Tissue Comment Organism Textmining
seed cotyledon and endosperm tissue Cyamopsis tetragonoloba
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Cyamopsis tetragonoloba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-mannoside + H2O
-
 Cyamopsis tetragonoloba 4-methylumbelliferone + beta-D-mannose
-
 ?
beta-D-mannohexaitol + H2O
-
 Cyamopsis tetragonoloba ?
-
 ?
beta-D-mannopentaitol + H2O
-
 Cyamopsis tetragonoloba ?
-
 ?
beta-D-mannotetraitol + H2O
-
 Cyamopsis tetragonoloba ?
-
 ?
beta-D-mannotriitol + H2O
-
 Cyamopsis tetragonoloba ?
-
 ?
beta-mannotetraitol + H2O
-
 Cyamopsis tetragonoloba ?
-
 ?
additional information the enzyme plays a key role in the hydrolysis of galactomannan in germinating guar seed Cyamopsis tetragonoloba ?
-
 ?
o-nitrophenyl-beta-D-mannopyranoside + H2O
-
 Cyamopsis tetragonoloba o-nitrophenol + alpha-D-mannose
-
 ?
p-nitrophenyl-beta-D-mannoside + H2O p-nitrophenyl-beta-D-mannopyranoside Cyamopsis tetragonoloba p-nitrophenol + alpha-D-mannose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 59000, SDS-PAGE Cyamopsis tetragonoloba

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
52
-
-
 Cyamopsis tetragonoloba

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 15 min, pH 5.5, stable up to Cyamopsis tetragonoloba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Cyamopsis tetragonoloba

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 40°C, 30 min, stable up to Cyamopsis tetragonoloba