3.2.1.25: beta-mannosidase
This is an abbreviated version!
For detailed information about beta-mannosidase, go to the full flat file.
Word Map on EC 3.2.1.25
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3.2.1.25
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lysosomal
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oligosaccharide
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alpha-mannosidase
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goat
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alpha-galactosidase
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glycosidases
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caprine
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mannans
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galactomannans
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alpha-fucosidase
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beta-hexosaminidase
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beta-xylosidase
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salers
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nubian
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1-4glcnac
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beta-fucosidase
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beta-glycosidases
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angiokeratomas
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beta-d-glucosidase
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medicine
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analysis
- 3.2.1.25
- lysosomal
- oligosaccharide
- alpha-mannosidase
- goat
- alpha-galactosidase
- glycosidases
-
caprine
- mannans
- galactomannans
- alpha-fucosidase
- beta-hexosaminidase
- beta-xylosidase
-
salers
-
nubian
-
1-4glcnac
- beta-fucosidase
-
beta-glycosidases
- angiokeratomas
- beta-d-glucosidase
- medicine
- analysis
Reaction
Synonyms
beta-D-mannosidase, beta-MAN, beta-mannosidase, beta-mannosidase 2A, beta-mannoside mannohydrolase, betaMANNOS1, BglB, CmMan5, CmMan5a, exo beta-mannanase, exo-beta-D-mannanase, GH2 beta-mannosidase, GM-1, GM-2, HvBII, Man2A, ManB, mannanase, mannase, mannosidase 5A, mannosidase, beta-, OT-1, PH0501, TM1624
ECTree
3.2.1.25 beta-mannosidaseAdvanced search results
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results (Turnover Number
Turnover Number on EC 3.2.1.25 - beta-mannosidase
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
2.17
4-O-beta-D-mannopyranosyl-D-mannopyranose
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pH 7.0, 37°C, wild-type enzyme
0.053
beta-D-mannosyl-1,4-beta-D-glucoside
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pH 7.0, 37°C, wild-type enzyme
additional information
additional information
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as the enzyme is inhibited by the substrate p-nitrophenyl-beta-mannoside at concentrations above 0.4 mM, the turnover number of 167 per s is only an estimate
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0.00183
2,4-dinitrophenyl-beta-D-mannopyranoside
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mutant enzyme E555A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.008
2,4-dinitrophenyl-beta-D-mannopyranoside
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mutant enzyme E462A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.85
2,4-dinitrophenyl-beta-D-mannopyranoside
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mutant enzyme N461A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
214.4
2,4-dinitrophenyl-beta-D-mannopyranoside
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wild type enzyme, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
322.25
4-nitrophenyl beta-D-fucopyranoside
80°C, pH is not specified in the publication, wild-type
323.41
4-nitrophenyl beta-D-fucopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
13.5
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, wild-type enzyme
14.2
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme Q77R
66.5
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme Q77R/D206N
571
4-nitrophenyl beta-D-galactopyranoside
pH 5.0, 90°C, mutant enzyme D206N
25.5
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme Q77R
34.4
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, wild-type enzyme
227.64
4-nitrophenyl beta-D-glucopyranoside
80°C, pH is not specified in the publication, wild-type
252
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme D206N
349.62
4-nitrophenyl beta-D-glucopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
439
4-nitrophenyl beta-D-glucopyranoside
pH 5.0, 90°C, mutant enzyme Q77R/D206N
1.6
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme Q77R
4.2
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, wild-type enzyme
6.8
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme Q77R/D206N
20.69
4-nitrophenyl beta-D-mannopyranoside
80°C, pH is not specified in the publication, mutant enzyme D206N
45
4-nitrophenyl beta-D-mannopyranoside
pH 5.0, 90°C, mutant enzyme D206N
50.99
4-nitrophenyl beta-D-mannopyranoside
80°C, pH is not specified in the publication, wild-type
0.0097
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme E555A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.045
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme D199A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.092
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme E462A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.25
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme N461A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.478
p-nitrophenyl-beta-D-mannopyranoside
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mutant enzyme W645A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
0.886
p-nitrophenyl-beta-D-mannopyranoside
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mutant enzyme W395A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
3.5
p-nitrophenyl-beta-D-mannopyranoside
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mutant enzyme W198A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
6.08
p-nitrophenyl-beta-D-mannopyranoside
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mutant enzyme W395A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
15.9
p-nitrophenyl-beta-D-mannopyranoside
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mutant enzyme Y537A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
20.38
p-nitrophenyl-beta-D-mannopyranoside
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mutant enzyme W198A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
26.85
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme Y537A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
34.43
p-nitrophenyl-beta-D-mannopyranoside
-
mutant enzyme Q646A, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
128.1
p-nitrophenyl-beta-D-mannopyranoside
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wild type enzyme, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
157
p-nitrophenyl-beta-D-mannopyranoside
-
wild type enzyme, at 37°C in 43.5 mM sodium phosphate, 10 mM citric acid buffer, pH 5.6, containing 1 mg/ml bovine serum albumin
