3.2.1.25: beta-mannosidase
This is an abbreviated version!
For detailed information about beta-mannosidase, go to the full flat file.
Word Map on EC 3.2.1.25
-
3.2.1.25
-
lysosomal
-
oligosaccharide
-
alpha-mannosidase
-
goat
-
alpha-galactosidase
-
glycosidases
-
caprine
-
mannans
-
galactomannans
-
alpha-fucosidase
-
beta-hexosaminidase
-
beta-xylosidase
-
salers
-
nubian
-
1-4glcnac
-
beta-fucosidase
-
beta-glycosidases
-
angiokeratomas
-
beta-d-glucosidase
-
medicine
-
analysis
- 3.2.1.25
- lysosomal
- oligosaccharide
- alpha-mannosidase
- goat
- alpha-galactosidase
- glycosidases
-
caprine
- mannans
- galactomannans
- alpha-fucosidase
- beta-hexosaminidase
- beta-xylosidase
-
salers
-
nubian
-
1-4glcnac
- beta-fucosidase
-
beta-glycosidases
- angiokeratomas
- beta-d-glucosidase
- medicine
- analysis
Reaction
Synonyms
beta-D-mannosidase, beta-MAN, beta-mannosidase, beta-mannosidase 2A, beta-mannoside mannohydrolase, betaMANNOS1, BglB, CmMan5, CmMan5a, exo beta-mannanase, exo-beta-D-mannanase, GH2 beta-mannosidase, GM-1, GM-2, HvBII, Man2A, ManB, mannanase, mannase, mannosidase 5A, mannosidase, beta-, OT-1, PH0501, TM1624
ECTree
3.2.1.25 beta-mannosidaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.2.1.25 - beta-mannosidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenyl-beta-D-mannopyranoside + H2O
2,4-dinitrophenol + alpha-D-mannose
-
-
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
-
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
-
129% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + alpha-D-mannose
-
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + methyl alpha-D-mannopyranoside
4-nitrophenyl alpha-D-mannopyranoside + methyl beta-D-mannopyranoside
-
the enzyme shows low transglycosylation activity at 50°C and pH 5.0
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
3.1% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
4-nitrophenyl beta-L-arabinopyranoside + H2O
4-nitrophenol + beta-L-arabinopyranose
-
16% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + D-xylose
?
-
transglycosylation reaction, 18.4% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + methanol
?
-
transglycosylation reaction, 42.6% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + N-acetyl-D-glucosamine
?
-
transglycosylation reaction, 1.4% transfer ratio
-
-
?
beta-D-Man-(1-4)-beta-D-GlcNAc-(1-4)-beta-D-GlcNAc-Asn-Lys + H2O
?
-
-
-
-
?
beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc(1->6)]-beta-D-GlcNAc-Asn-peptide + H2O
beta-D-GlcNAc-(1->4)-[alpha-L-Fuc(1->6)]-beta-D-GlcNAc-Asn-peptide + D-mannopyranose
-
-
-
-
?
beta-D-mannosyl-1,4-beta-D-glucoside + H2O
D-mannose + beta-D-glucose
-
-
-
-
?
cellobiose + H2O
?
-
33% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
cellohexaose + H2O
?
-
303% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
cellopentaose + H2O
?
-
225% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
cellotetraose + H2O
?
-
52% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
cellotriose + H2O
?
-
19% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
laminaribiose + H2O
?
-
13% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
laminaritriose + H2O
?
-
2.8% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
Manbeta1-4GlcNAcbeta1-4Manbeta1-4GlcNAcbeta1-4GlcNAc + H2O
mannose + GlcNAcbeta1-4Manbeta1-4GlcNAcbeta1-4GlcNAc
-
-
-
?
mannobiose + glucose + D-glucose
?
-
transglycosylation reaction, 40.1% transfer ratio
-
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
-
4.6% activity compared to p-nitrophenyl-beta-D-mannoside
-
-
?
p-nitrophenyl-beta-D-glucoside + H2O
p-nitrophenol + beta-D-glucose
-
34.5% activity compared to p-nitrophenyl-beta-D-mannoside
-
-
?
p-nitrophenyl-beta-D-mannopyranoside + H2O
p-nitrophenol + alpha-D-mannose
-
self-transfer of the mannosyl group is observed, and a 10-15% yield of a beta1,4-disaccharide is obtained
-
-
?
p-nitrophenyl-beta-galactoside + H2O
p-nitrophenol + beta-D-galactopyranose
-
low activity
-
-
?
p-nitrophenyl-beta-mannoside + H2O
p-nitrophenol + beta-D-mannopyranose
-
-
-
-
?
sophorose + H2O
?
-
15% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
4-methylumbelliferyl beta-D-mannoside + H2O
4-methylumbelliferone + beta-D-mannose
-
4-methylumbelliferyl beta-D-mannopyranoside
-
-
?
4-methylumbelliferyl beta-D-mannoside + H2O
4-methylumbelliferone + beta-D-mannose
-
-
-
-
?
4-methylumbelliferyl beta-D-mannoside + H2O
4-methylumbelliferone + beta-D-mannose
-
-
-
-
?
4-methylumbelliferyl beta-D-mannoside + H2O
4-methylumbelliferone + beta-D-mannose
-
-
-
-
?
4-nitrophenol beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
-
-
-
-
?
4-nitrophenol beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
-
17% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-galactopyranoside is 20% compared to catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl-beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-galactopyranoside is 20% compared to catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl-beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranose + H2O
4-nitrophenol + beta-D-glucose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranose + H2O
4-nitrophenol + beta-D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-glucopyranoside is 26% compared to catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-mannopyranoside. The catalytic efficiency (kcat/Km) of the mutant enzymes Q77R, D206N and Q77R/D206N for 4-nitrophenyl beta-D-glucopyranoside is higher then that for 4-nitrophenyl beta-D-galactopyranoside and 4-nitrophenyl-beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-glucopyranoside is 26% compared to catalytic efficiency (kcat/Km) of the wild-type enzyme for 4-nitrophenyl beta-D-mannopyranoside. The catalytic efficiency (kcat/Km) of the mutant enzymes Q77R, D206N and Q77R/D206N for 4-nitrophenyl beta-D-glucopyranoside is higher then that for 4-nitrophenyl beta-D-galactopyranoside and 4-nitrophenyl-beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranose
-
activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannose
-
238% of the activity with 4-nitrophenyl-beta-D-glucopyranose
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannopyranose
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannopyranose
-
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
-
-
-
-
?
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
-
-
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + 1-propanol
?
-
transglycosylation reaction, 50% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + 1-propanol
?
-
transglycosylation reaction, 50% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + D-fructose
?
-
transglycosylation reaction, 72.3% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + D-fructose
?
-
transglycosylation reaction, 72.3% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + D-galactose
?
-
transglycosylation reaction, 14.2% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + D-galactose
?
-
transglycosylation reaction, 14.2% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + ethanol
?
-
transglycosylation reaction, 41.1% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + ethanol
?
-
transglycosylation reaction, 41.1% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + H2O
D-mannopyranose
-
-
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + H2O
D-mannopyranose
Bacillus sp. (in: Bacteria) AM-001
-
-
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + L-arabinose
?
-
transglycosylation reaction, 6.4% transfer ratio
-
-
?
4-O-beta-D-mannopyranosyl-D-mannopyranose + L-arabinose
?
-
transglycosylation reaction, 6.4% transfer ratio
-
-
?
Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta-Asn + H2O
mannose + GlcNAcbeta(1-4)GlcNAcbeta-Asn
-
-
-
?
Manbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta-Asn + H2O
mannose + GlcNAcbeta(1-4)GlcNAcbeta-Asn
Polyporus sulfureus
-
-
-
-
?
mannan + H2O
mannose + ?
-
glucomannans and galactomannans are completely hydrolyzed
-
-
?
mannobiose + H2O
2 D-mannopyranose
activity is 15% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside
-
-
?
mannobiose + H2O
2 D-mannopyranose
activity is 15% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside
-
-
?
mannotetraose + H2O
4 D-mannopyranose
activity is 32% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside
-
-
?
mannotetraose + H2O
4 D-mannopyranose
activity is 32% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside
-
-
?
mannotriose + H2O
3 D-mannopyranose
activity is 47% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside
-
-
?
mannotriose + H2O
3 D-mannopyranose
activity is 47% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside
-
-
?
o-nitrophenyl-beta-D-mannopyranoside + H2O
o-nitrophenol + alpha-D-mannose
-
-
-
?
o-nitrophenyl-beta-D-mannopyranoside + H2O
o-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannopyranose
-
-
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannopyranose
-
-
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannose
-
-
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannose
-
-
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannose
-
-
-
-
?
p-nitrophenyl beta-D-mannopyranoside + H2O
p-nitrophenol + beta-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
no activity with p-nitrophenyl-alpha-D-mannoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
Bacillus sp. (in: Bacteria) AM-001
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
Cutaneotrichosporon cutaneum JCM 2947
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
hydrolyses 4-nitrophenyl-beta-D-mannoside three times faster than p-nitrophenyl-beta-D-glucoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
p-nitrophenyl-beta-D-mannopyranoside
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
Phlebia radiata 79 / ATCC 64658
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
Polyporus sulfureus
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
p-nitrophenyl-beta-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
-
-
-
-
?
additional information
?
-
-
hydrolyses D-galactose substituted manno-oligosaccharides up to, but not beyond, a side group
-
-
?
additional information
?
-
-
locust bean gum and guar flour are the best substrates for enzyme formation
-
-
?
additional information
?
-
-
in 4 cultures induced by locust bean gum
-
-
?
additional information
?
-
-
in 4 cultures induced by locust bean gum
-
-
?
additional information
?
-
-
the Michaelis complex of the beta-mannosidase Man2A shows distortion to a 1S5 conformation supporting catalysis through a boat conformation
-
-
?
additional information
?
-
-
deficiency of lysosomal beta-mannosidase activity results in a severe neurodegenerative disease in goats and cattle and a relatively milder phenotype in humans
-
-
?
additional information
?
-
-
in 4 cultures induced by locust bean gum
-
-
?
additional information
?
-
-
goat liver contains both a lysosomal beta-mannosidase, acidic enzyme form, deficient in beta-mannosidosis, and a neutral nonlysosomal
-
-
?
additional information
?
-
-
Glu519 is the catalytic nucleophile in beta-mannosidase 2A
-
-
?
additional information
?
-
-
no hydrolysis of p-nitrophenyl derivatives of alpha-mannose, beta-N-acetylglucosamine, beta-glucose, beta-xylose, beta-cellobiose or beta-gentiobiose
-
-
?
additional information
?
-
-
CmMan5A releases beta-1,4-linked mannose substrates exclusively from the nonreducing end
-
-
?
additional information
?
-
-
the enzyme plays a key role in the hydrolysis of galactomannan in germinating guar seed
-
-
?
additional information
?
-
no substrate: 4-nitrophenyl-alpha-D-mannose
-
-
?
additional information
?
-
-
4-nitrophenyl-N-acetyl-beta-D-glucosaminide, 4-nitrophenyl-beta-D-xyloside, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-gentiobioside, and 4-nitrophenyl-cellobioside are no substrates
-
-
?
additional information
?
-
-
comparison with the activities of rice family GH1 glycoside hydrolases
-
-
?
additional information
?
-
in glycoside recognition and substrate specificity of HvBII, a combination of the following determinants is likely to play key roles: the inherent conformational and spatial flexibilities of gluco- and manno-configured substrates in the enzymes' active sites, the subtle differences in the spatial disposition of active site residues and their capacities to form interactions with specific groups of substrates, and the small variations in the charge distributions and shapes of the catalytic sites
-
-
?
additional information
?
-
-
in glycoside recognition and substrate specificity of HvBII, a combination of the following determinants is likely to play key roles: the inherent conformational and spatial flexibilities of gluco- and manno-configured substrates in the enzymes' active sites, the subtle differences in the spatial disposition of active site residues and their capacities to form interactions with specific groups of substrates, and the small variations in the charge distributions and shapes of the catalytic sites
-
-
?
additional information
?
-
-
in 4 cultures induced by locust bean gum
-
-
?
additional information
?
-
Polyporus sulfureus
-
the enzyme liberates mannose from core glycopeptide isolated from Taka-amylase A and alpha1-acid glycoprotein
-
-
?
additional information
?
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extensively hydrolyzes the carbohydrate portion of glycopeptides obtained from ovalbumin
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beta-mannosidase and endo-beta-mannanase are involved in the mobilization of the mannan-containing cell walls of the tomato seed endosperm. A lack of coordination of the activities of these two enzymes within individuals of a population
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coincidental and substantial increase in activity of both beta-mannosidase and beta-mannanase after 48 h, following germination, suggests the cooperation of both enzymes in the degradation of endosperm cell wall galactomannan
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coincidental and substantial increase in activity of both beta-mannosidase and beta-mannanase after 48 h, following germination, suggests the cooperation of both enzymes in the degradation of endosperm cell wall galactomannan
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calculation of conformational free-energy surface of a beta-D-mannopyranose molecule associated with the ideal Stoddart conformational diagram. 1S5 is among the most stable conformers and simultaneously is the most preactivated conformation in terms of elongation/shortening of the C1-O1/C1-O5 bonds, C1-O1 orientation, and charge development at the anomeric carbon. Analysis of the computed free-energy surface gives support to the proposed 1S5-B2,5-OS2 catalytic itinerary. The degree of preactivation of the substrate in glycoside hydrolases is related to the properties of an isolated sugar ring. Introduction of a simple preactivation index integrating several structural, electronic, and energetic properties that can be used to predict the conformation of the substrate in the Michaelis complex of any glycoside hydrolase
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the enzyme also exhibited transglycosidase activity when the reactionwas carried out with 10% (w/v) of mannobiose in the presence of alcohols or galactose
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the enzyme also exhibited transglycosidase activity when the reactionwas carried out with 10% (w/v) of mannobiose in the presence of alcohols or galactose
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in addition to hydrolysis, enzyme catalyzes transglycosylation with substrate 4-nitrophenyl-beta-D-mannopyranoside to yield 4-nitrophenyl-mannobiosides and 4-nitrophenyl mannotriosides containing beta-(1,4) and beta-(1,3) linkages. The rate of donor substrate hydrolysis increases in presence of acetonitrile and dimethylformamide
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